FastQCFastQC Report
Mon 6 Feb 2017
SRR2077504_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077504_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3312760
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32650.034.6842541
GTATCAA63250.031.8236311
TCAACGC78500.025.0077954
TATCAAC79000.024.9805342
ATCAACG79150.024.8903583
CAACGCA81100.024.121355
AACGCAG84800.023.131016
ACGCAGA96700.020.002017
GTACATG93100.019.7289371
CGCAGAG100150.019.211158
TACATGG93500.018.8925742
AGAGTAC95550.018.58487911
GTATACG1150.001105299618.0133741
ACATGGG97400.017.6761443
CAGAGTA104500.017.32286310
GTGTAGC13800.017.262821
GCGATAC3205.456968E-1217.24089414
GAGTACT53150.017.06196812
TAGGTCG1855.4507236E-616.7845675
GCAGAGT110900.016.5408089
Produced by FastQC (version 0.11.5)