##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077504_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3312760 Sequences flagged as poor quality 0 Sequence length 75 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.574250172061966 31.0 30.0 34.0 25.0 34.0 2 29.621228522440504 31.0 30.0 34.0 25.0 34.0 3 29.664940110361147 31.0 30.0 34.0 24.0 34.0 4 33.07088862459098 37.0 35.0 37.0 26.0 37.0 5 33.06725177797365 37.0 35.0 37.0 26.0 37.0 6 33.1168267547302 37.0 35.0 37.0 26.0 37.0 7 32.96851990485275 37.0 35.0 37.0 25.0 37.0 8 32.908563252393776 37.0 35.0 37.0 25.0 37.0 9 34.298582450886876 39.0 35.0 39.0 25.0 39.0 10 34.19931416703896 39.0 35.0 39.0 25.0 39.0 11 34.2843154952366 39.0 35.0 39.0 25.0 39.0 12 34.1906196041971 39.0 35.0 39.0 24.0 39.0 13 34.17567798452046 39.0 35.0 39.0 23.0 39.0 14 35.25364016711141 40.0 36.0 41.0 23.0 41.0 15 35.28038070974052 40.0 36.0 41.0 24.0 41.0 16 35.25991439162511 40.0 36.0 41.0 23.0 41.0 17 35.17892693705551 40.0 36.0 41.0 23.0 41.0 18 35.13636876803632 40.0 36.0 41.0 22.0 41.0 19 35.223771417186875 40.0 36.0 41.0 22.0 41.0 20 35.20562521885075 40.0 36.0 41.0 22.0 41.0 21 35.17828457238073 40.0 36.0 41.0 22.0 41.0 22 35.103660391939044 40.0 35.0 41.0 22.0 41.0 23 35.05038457358819 40.0 35.0 41.0 22.0 41.0 24 35.016992175708474 40.0 35.0 41.0 22.0 41.0 25 35.03870367910745 40.0 35.0 41.0 22.0 41.0 26 34.97455897801229 40.0 35.0 41.0 22.0 41.0 27 34.96111490117002 40.0 35.0 41.0 22.0 41.0 28 34.91733539405209 40.0 35.0 41.0 22.0 41.0 29 34.88018993226192 40.0 35.0 41.0 21.0 41.0 30 34.789844117895655 40.0 35.0 41.0 20.0 41.0 31 34.77363406947681 40.0 35.0 41.0 20.0 41.0 32 34.70357073859863 40.0 35.0 41.0 19.0 41.0 33 34.59545605477004 40.0 35.0 41.0 18.0 41.0 34 34.55202006785883 39.0 34.0 41.0 18.0 41.0 35 34.504958101401854 39.0 34.0 41.0 18.0 41.0 36 34.42987116482933 39.0 34.0 41.0 16.0 41.0 37 34.3544364819667 39.0 34.0 41.0 16.0 41.0 38 34.28013982298748 39.0 34.0 41.0 16.0 41.0 39 34.23207868967266 39.0 34.0 41.0 15.0 41.0 40 34.19091452444487 39.0 34.0 41.0 15.0 41.0 41 34.10551534068269 39.0 34.0 41.0 15.0 41.0 42 34.02217667443461 39.0 34.0 41.0 13.0 41.0 43 33.92068215023123 39.0 33.0 41.0 9.0 41.0 44 33.82557595479298 39.0 33.0 41.0 9.0 41.0 45 33.72587268621935 39.0 33.0 41.0 9.0 41.0 46 33.631551334838626 39.0 33.0 41.0 8.0 41.0 47 33.523644030959076 39.0 33.0 41.0 7.0 41.0 48 33.42024897668409 39.0 33.0 41.0 2.0 41.0 49 33.31439162511018 39.0 33.0 40.0 2.0 41.0 50 33.19296357116121 38.0 33.0 40.0 2.0 41.0 51 33.089580893273286 38.0 33.0 40.0 2.0 41.0 52 32.985372317946364 38.0 33.0 40.0 2.0 41.0 53 32.89900354991004 38.0 33.0 40.0 2.0 41.0 54 32.89295632644683 38.0 33.0 40.0 2.0 41.0 55 32.79045569253432 38.0 32.0 40.0 2.0 41.0 56 32.651315821248744 38.0 32.0 40.0 2.0 41.0 57 32.55072115094362 38.0 32.0 40.0 2.0 41.0 58 32.44754011760586 38.0 32.0 40.0 2.0 41.0 59 32.328858112268925 37.0 32.0 40.0 2.0 41.0 60 32.292210422729084 37.0 32.0 40.0 2.0 41.0 61 32.174894347915334 37.0 32.0 40.0 2.0 41.0 62 32.013496299158405 37.0 32.0 40.0 2.0 41.0 63 31.823246779120733 37.0 31.0 40.0 2.0 41.0 64 31.67652803100738 36.0 31.0 40.0 2.0 41.0 65 31.51660639466789 36.0 31.0 40.0 2.0 41.0 66 31.343828107076877 36.0 31.0 39.0 2.0 41.0 67 31.16949280962098 36.0 31.0 39.0 2.0 41.0 68 30.979146693391613 35.0 31.0 39.0 2.0 41.0 69 31.01188887815598 35.0 31.0 39.0 2.0 41.0 70 30.90663495091706 35.0 31.0 39.0 2.0 41.0 71 30.740812192854296 35.0 31.0 39.0 2.0 41.0 72 30.536836655839842 35.0 31.0 38.0 2.0 40.0 73 30.326985051739335 35.0 31.0 37.0 2.0 40.0 74 30.108946014803365 35.0 31.0 37.0 2.0 40.0 75 29.888374044603292 35.0 31.0 37.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7341041695600055 1101 2 0.8055743228307364 1101 3 0.7823383206779795 1101 4 0.8415391992512795 1101 5 0.8787261268390409 1101 6 0.7454675332974716 1101 7 0.7072686196171034 1101 8 0.728551431053738 1101 9 0.8980315686012474 1101 10 0.937702079097221 1101 11 0.9006039633795524 1101 12 0.8665614160806285 1101 13 0.8958948093103558 1101 14 1.0174154588180926 1101 15 1.0679681469451054 1101 16 1.078613030332889 1101 17 1.066861688380854 1101 18 1.127934874665229 1101 19 1.1135695892779225 1101 20 1.0852393903181792 1101 21 1.0969056821151142 1101 22 1.1526517693370977 1101 23 1.113850000949995 1101 24 1.1705216033715118 1101 25 1.0702164534225815 1101 26 1.0309521220811746 1101 27 1.145454318729989 1101 28 1.0555826027986797 1101 29 1.129735437810936 1101 30 1.0653160814360092 1101 31 1.1034209671522177 1101 32 1.1269658880033901 1101 33 1.1652370412526878 1101 34 1.0778888695717868 1101 35 1.0870225123187183 1101 36 1.1554157476988252 1101 37 1.1349068643268865 1101 38 1.097928961722296 1101 39 1.0818844388364894 1101 40 1.1739607851462424 1101 41 1.1662363490017569 1101 42 1.0215964416320915 1101 43 1.0392311868808264 1101 44 1.0926430065967025 1101 45 1.049184442706803 1101 46 1.0858869361186052 1101 47 1.1016963845513317 1101 48 1.1184184359760465 1101 49 1.1499864732778207 1101 50 1.1188912365121126 1101 51 1.0215040275728953 1101 52 1.0415414568151604 1101 53 1.0779018179949205 1101 54 1.0331463883651537 1101 55 0.8930988950357843 1101 56 0.9477287026187327 1101 57 0.9026633161121254 1101 58 0.9706741020703618 1101 59 1.0318399981529112 1101 60 1.0439955828575123 1101 61 1.0364982069774769 1101 62 0.9696631722241413 1101 63 1.0979774425380207 1101 64 1.05842378020321 1101 65 1.0607227084759785 1101 66 0.9402149374649689 1101 67 0.923021540265875 1101 68 0.9774793190830131 1101 69 1.0051372952971036 1101 70 1.0885565547487523 1101 71 1.1303813625236394 1101 72 1.119472057123435 1101 73 1.0668637916770152 1101 74 1.0787921162243705 1101 75 1.0928319703084206 1102 1 0.8141906509921846 1102 2 0.8766029739917904 1102 3 0.9469372589790765 1102 4 0.9110256108657424 1102 5 0.8531198659645511 1102 6 0.9074379916324844 1102 7 0.9114754306082489 1102 8 0.9964867636875141 1102 9 1.140989845781732 1102 10 1.1199585207484972 1102 11 1.0836550477122486 1102 12 1.104934021016966 1102 13 1.0588765800492723 1102 14 1.2701511514516284 1102 15 1.2887364664132974 1102 16 1.2336647323900962 1102 17 1.310561260876817 1102 18 1.2646404348419225 1102 19 1.2376994143227265 1102 20 1.232899935676734 1102 21 1.3867621207278304 1102 22 1.4355936814793395 1102 23 1.3988371112595672 1102 24 1.4118264574984067 1102 25 1.4545940657602188 1102 26 1.4892200228847727 1102 27 1.4888387101539422 1102 28 1.4105365099839346 1102 29 1.4605747817868107 1102 30 1.4848317823081771 1102 31 1.523298532357721 1102 32 1.525817383093532 1102 33 1.4854985866412846 1102 34 1.5241144657415546 1102 35 1.5024158774947338 1102 36 1.554247172784791 1102 37 1.460242624706403 1102 38 1.4339404536772022 1102 39 1.4610668118999754 1102 40 1.452178736241386 1102 41 1.4325195102596169 1102 42 1.4891128161658216 1102 43 1.474439386629939 1102 44 1.4258726233278196 1102 45 1.5021883482864524 1102 46 1.4549157861669286 1102 47 1.3929675210712062 1102 48 1.441904821003149 1102 49 1.3840515373827103 1102 50 1.3884535343230056 1102 51 1.418641760113026 1102 52 1.3912770765924307 1102 53 1.4401861107694103 1102 54 1.3024716874815923 1102 55 1.4226456962137703 1102 56 1.433543137345552 1102 57 1.4304300589843777 1102 58 1.3943398913541358 1102 59 1.3410067589112131 1102 60 1.3143270150219308 1102 61 1.350806341708406 1102 62 1.3250061353612814 1102 63 1.3046098322272321 1102 64 1.2221133530893447 1102 65 1.3522781484743476 1102 66 1.335340792360821 1102 67 1.4204062244960802 1102 68 1.2674418913307122 1102 69 1.2662897002619644 1102 70 1.318663598223921 1102 71 1.271554505629588 1102 72 1.3212835023260219 1102 73 1.2683107397070117 1102 74 1.3103264607060936 1102 75 1.2617229545345197 1103 1 0.8489421932605055 1103 2 0.885162393692152 1103 3 0.9095255580774406 1103 4 0.9758483347488678 1103 5 0.9950654967692429 1103 6 0.9482596308433884 1103 7 0.997079118493815 1103 8 0.9207251204090952 1103 9 1.047721592781933 1103 10 0.9987830072239845 1103 11 0.9635926790592748 1103 12 0.8869325295926558 1103 13 1.0439487785442907 1103 14 1.1033055138677383 1103 15 1.1220142102950703 1103 16 1.2058578040462535 1103 17 1.229242028282414 1103 18 1.2135984715851933 1103 19 1.2182105661090503 1103 20 1.2391612060990838 1103 21 1.205375096119333 1103 22 1.2009845024406332 1103 23 1.200220068347221 1103 24 1.2757692410385033 1103 25 1.3191293515035625 1103 26 1.3020693212374255 1103 27 1.3306962113403031 1103 28 1.2687793409784405 1103 29 1.2037176195605213 1103 30 1.250914081813292 1103 31 1.2623399532783353 1103 32 1.2059177152743956 1103 33 1.2762287066331481 1103 34 1.2284301053592017 1103 35 1.2739418757321417 1103 36 1.2686977185105093 1103 37 1.3538335676933215 1103 38 1.2964745191642848 1103 39 1.4179836310986715 1103 40 1.3776793802655192 1103 41 1.3563036604867236 1103 42 1.3960642092917084 1103 43 1.3439492503679844 1103 44 1.434555424493844 1103 45 1.3580191394192553 1103 46 1.454325995644247 1103 47 1.439224723972707 1103 48 1.379624297648796 1103 49 1.340910055449278 1103 50 1.3754984804283552 1103 51 1.4535166838470133 1103 52 1.3698466650949044 1103 53 1.3389875479601088 1103 54 1.2958158675371863 1103 55 1.3049235078875583 1103 56 1.356696821841517 1103 57 1.2382966443006325 1103 58 1.337912530497242 1103 59 1.3447556572909818 1103 60 1.3789029759737303 1103 61 1.3365157851359655 1103 62 1.2981930379441557 1103 63 1.3571513548358745 1103 64 1.3482932730947716 1103 65 1.4376594785536625 1103 66 1.3559305828834987 1103 67 1.3528569058058189 1103 68 1.4131309971540418 1103 69 1.4095694779041565 1103 70 1.4038754482679892 1103 71 1.2975893507248344 1103 72 1.373804687330427 1103 73 1.4138778199049646 1103 74 1.4267538215629862 1103 75 1.3485875738823623 1104 1 0.6328804252431652 1104 2 0.5959612189138248 1104 3 0.5456144918419028 1104 4 0.6536485503118783 1104 5 0.6345489468992582 1104 6 0.5314892294875051 1104 7 0.5168225263927155 1104 8 0.6070157939032583 1104 9 0.7293282183393686 1104 10 0.7792246309182858 1104 11 0.7187950799022929 1104 12 0.7609662423649652 1104 13 0.6985242836949652 1104 14 0.8017359106559283 1104 15 0.81669917978369 1104 16 0.8112040871870647 1104 17 0.84118117380347 1104 18 0.8706035882015755 1104 19 0.8155261255110844 1104 20 0.8207929138950121 1104 21 0.8598955485612478 1104 22 0.8698704263364689 1104 23 0.9047072247185497 1104 24 0.9800809820102998 1104 25 0.9477137275928982 1104 26 0.9802319070319001 1104 27 0.9176207522672186 1104 28 0.9007144797521391 1104 29 0.8853378680589898 1104 30 0.8871157654485344 1104 31 0.9546922745874227 1104 32 0.961473511234793 1104 33 1.0079239213747755 1104 34 1.0177695930564852 1104 35 0.9697311047938797 1104 36 0.9185883743749841 1104 37 1.0129537661074508 1104 38 0.9873220664338902 1104 39 1.0405343264696754 1104 40 1.012733851995911 1104 41 1.099435254090352 1104 42 1.0724441931845874 1104 43 1.0414836583109661 1104 44 1.0985463051368427 1104 45 1.0547606921941295 1104 46 1.1445114679796617 1104 47 1.1355819495405655 1104 48 1.0543297015489657 1104 49 1.0549325493289743 1104 50 1.0111324880972674 1104 51 1.0041228646554927 1104 52 0.9710550199645027 1104 53 1.0059511947412858 1104 54 1.039178570328687 1104 55 0.8822901132509884 1104 56 1.0402368632712822 1104 57 0.9299459417174774 1104 58 0.9618790501162806 1104 59 0.9341794469527755 1104 60 1.0252965427401648 1104 61 1.0560022002590372 1104 62 1.0464213744628559 1104 63 0.930185704681012 1104 64 0.9999000989895812 1104 65 0.9124857259542836 1104 66 1.0035997377402772 1104 67 1.0143435693472291 1104 68 1.0059002605936769 1104 69 1.0018705929819447 1104 70 1.0296358688161895 1104 71 1.0219545417064495 1104 72 1.0233586962815018 1104 73 1.0884917096953721 1104 74 1.0560949119592138 1104 75 1.068939206030901 1105 1 0.4553979705455511 1105 2 0.46434445824006687 1105 3 0.49381627007547024 1105 4 0.44536693538942274 1105 5 0.40464815259579723 1105 6 0.4520467705329665 1105 7 0.44524369104863126 1105 8 0.47000523866481814 1105 9 0.6345479046814191 1105 10 0.6367992673865928 1105 11 0.5818828983251052 1105 12 0.5885033598464133 1105 13 0.5285844030823554 1105 14 0.6032759499357354 1105 15 0.6174209607262142 1105 16 0.6375848304074268 1105 17 0.6824265564913361 1105 18 0.7288189692447133 1105 19 0.7453078807185562 1105 20 0.6835169587475178 1105 21 0.6829745048406934 1105 22 0.7371658278902515 1105 23 0.7238091296923841 1105 24 0.825819006990713 1105 25 0.7251663064185649 1105 26 0.7303826989166566 1105 27 0.8534594535181768 1105 28 0.8250868058712726 1105 29 0.8256235077103113 1105 30 0.7903829165016418 1105 31 0.7079965189348982 1105 32 0.8420527238972113 1105 33 0.8191200591830423 1105 34 0.7973584128240958 1105 35 0.8535063004672949 1105 36 0.8809480264681326 1105 37 0.8375211754784857 1105 38 0.8567997224163193 1105 39 0.8883221381201025 1105 40 0.8853640316574882 1105 41 0.854208563172115 1105 42 0.8808062639849865 1105 43 0.8158102998875734 1105 44 0.838838252776668 1105 45 0.8194293389757945 1105 46 0.8544472077654603 1105 47 0.8750631078110942 1105 48 0.8123821197724723 1105 49 0.7705616937838471 1105 50 0.8800513259568064 1105 51 0.807485641718408 1105 52 0.8507611760581923 1105 53 0.760008489078615 1105 54 0.75935368473354 1105 55 0.7704726579245147 1105 56 0.8220193150664485 1105 57 0.7016344122237612 1105 58 0.7250384235743539 1105 59 0.7200983525361124 1105 60 0.7906280314061078 1105 61 0.8277816141578924 1105 62 0.8146453661038002 1105 63 0.8748138332788393 1105 64 0.8278951454771324 1105 65 0.9166158717539545 1105 66 0.8459409302597045 1105 67 0.8584385761679805 1105 68 0.8827767227022107 1105 69 0.910144003649414 1105 70 0.8485386658092509 1105 71 0.866129385216091 1105 72 0.847993403763045 1105 73 0.788398637840416 1105 74 0.8252036860616307 1105 75 0.7929147480631933 1106 1 0.34722023965076687 1106 2 0.40789917116884666 1106 3 0.36498925406714733 1106 4 0.4165215779937057 1106 5 0.2991008237385273 1106 6 0.3367958403965474 1106 7 0.3017868622079831 1106 8 0.4253791416265287 1106 9 0.46028203059076134 1106 10 0.5240469854043894 1106 11 0.4751286111445907 1106 12 0.46200446460614586 1106 13 0.47039795765272885 1106 14 0.5855473359459111 1106 15 0.5867756765059795 1106 16 0.5266815883533837 1106 17 0.4429988838043215 1106 18 0.5614656884646863 1106 19 0.5347465867479357 1106 20 0.6287258177326578 1106 21 0.6420738191769075 1106 22 0.7108941187675484 1106 23 0.6292691801549566 1106 24 0.7134435882511383 1106 25 0.5404445490665708 1106 26 0.7134994613291639 1106 27 0.6901034928048588 1106 28 0.6823028873716552 1106 29 0.590148261455802 1106 30 0.6609069384922961 1106 31 0.6104239492875223 1106 32 0.570019044168248 1106 33 0.7263149578064869 1106 34 0.6588769466534998 1106 35 0.6059326189327052 1106 36 0.621940609135045 1106 37 0.5730565396690679 1106 38 0.5820930658231589 1106 39 0.6115220544909832 1106 40 0.5170606860173663 1106 41 0.5168698129523506 1106 42 0.6992057356009767 1106 43 0.6075764044334804 1106 44 0.7055345317558235 1106 45 0.6249252549285274 1106 46 0.6241912171167172 1106 47 0.6732053248381717 1106 48 0.6179886542481086 1106 49 0.6368077819840607 1106 50 0.6218066822596384 1106 51 0.646550706127087 1106 52 0.6836845176118871 1106 53 0.7393170852972446 1106 54 0.6278328225932981 1106 55 0.5699246590839167 1106 56 0.560050286352201 1106 57 0.569559241045404 1106 58 0.5802366483561343 1106 59 0.5560167191072765 1106 60 0.5172009764342107 1106 61 0.667276342196935 1106 62 0.6514820944944653 1106 63 0.7377571792990913 1106 64 0.6887999175640083 1106 65 0.6352228386301704 1106 66 0.6547393603129663 1106 67 0.7113121773996092 1106 68 0.6998772439587384 1106 69 0.5983817384405548 1106 70 0.6470999787864962 1106 71 0.5702424745994676 1106 72 0.5736886231231892 1106 73 0.5941897646003085 1106 74 0.6873824338105337 1106 75 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-1.7016195171254438 2215 19 -1.49670736318388 2215 20 -1.5054886007933703 2215 21 -1.8220602871248772 2215 22 -1.5313680673751762 2215 23 -1.6728859922588342 2215 24 -1.4250369829662475 2215 25 -1.4746886074982228 2215 26 -1.5600452063918127 2215 27 -1.541977956811813 2215 28 -1.6134610779163907 2215 29 -1.5863651938678842 2215 30 -1.6750826502128504 2215 31 -1.6113417163592132 2215 32 -1.6866673263179948 2215 33 -1.5734604644898411 2215 34 -1.6929446718243994 2215 35 -1.6762879965197897 2215 36 -1.698987590461563 2215 37 -1.8260713639763182 2215 38 -1.564910284757282 2215 39 -1.6843035792400087 2215 40 -1.6447333499780967 2215 41 -1.6867229289369234 2215 42 -1.6544415839346698 2215 43 -1.6946525767264902 2215 44 -1.6491470413409743 2215 45 -1.5378932937358272 2215 46 -1.4313469098815972 2215 47 -1.560319245130085 2215 48 -1.50723696200658 2215 49 -1.4628949059292111 2215 50 -1.527013774473609 2215 51 -1.458826230591491 2215 52 -1.3703880348456785 2215 53 -1.4666649327291346 2215 54 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-1.757379729098865 2216 16 -1.7224250242365713 2216 17 -1.7129801939398064 2216 18 -1.7207449627582392 2216 19 -1.7791631712646847 2216 20 -1.7529600060221284 2216 21 -1.8471501564059167 2216 22 -1.8232579218017904 2216 23 -1.9327092245820694 2216 24 -1.824230758961491 2216 25 -1.8992544868802739 2216 26 -1.8694458302777264 2216 27 -1.8305159098718207 2216 28 -1.8960691438700437 2216 29 -2.0164844006415024 2216 30 -1.9648434939442794 2216 31 -1.862306511368132 2216 32 -1.8664055170488325 2216 33 -1.7872056368011897 2216 34 -1.9479335310044306 2216 35 -1.9777752959850332 2216 36 -1.970494200681415 2216 37 -2.0277825939228435 2216 38 -1.9512022485124803 2216 39 -1.9145416214265794 2216 40 -2.0829266803405417 2216 41 -1.955817551634489 2216 42 -1.912218618991119 2216 43 -2.055242668823933 2216 44 -2.0401920502536335 2216 45 -2.1456172242171085 2216 46 -1.9604214791032248 2216 47 -1.8504722457242622 2216 48 -2.01875954073504 2216 49 -2.0726252980860735 2216 50 -2.163087378157506 2216 51 -2.1662812959509665 2216 52 -1.9806583854101483 2216 53 -2.0448508358782753 2216 54 -2.08721443549312 2216 55 -2.178510835546785 2216 56 -2.112596107451413 2216 57 -2.0661478001438134 2216 58 -2.0804713078865937 2216 59 -2.042315049779724 2216 60 -2.1047333876626304 2216 61 -2.2297468411266586 2216 62 -2.0872615075103873 2216 63 -2.0773940997095828 2216 64 -2.183019858218362 2216 65 -2.318704157809975 2216 66 -2.213160326207408 2216 67 -2.17946632651741 2216 68 -2.126060922037876 2216 69 -2.0172992089645305 2216 70 -2.046831622439086 2216 71 -1.9737237805882941 2216 72 -2.010639757114017 2216 73 -2.01016258413544 2216 74 -2.2209229461137845 2216 75 -2.1249477796529916 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 153571.0 3 22668.0 4 15456.0 5 11418.0 6 9504.0 7 8244.0 8 8127.0 9 8360.0 10 9083.0 11 9657.0 12 10198.0 13 10600.0 14 11052.0 15 11345.0 16 12212.0 17 12798.0 18 13314.0 19 14275.0 20 15567.0 21 18032.0 22 21573.0 23 25289.0 24 27689.0 25 29773.0 26 32527.0 27 35749.0 28 42081.0 29 49407.0 30 55376.0 31 63485.0 32 76502.0 33 95629.0 34 124177.0 35 162978.0 36 227507.0 37 353001.0 38 576773.0 39 796530.0 40 131233.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.38741224141195 17.68681457268894 13.68051065260876 24.245262533290347 2 15.783088211958482 19.319437194593196 39.60792468604324 25.289549907405085 3 19.392455215176017 23.868534661898646 28.750667612532947 27.98834251039239 4 13.096525119870364 15.912012431448662 36.74348648926457 34.2479759594164 5 14.540805914891802 37.07551941383171 33.77992507827902 14.60374959299747 6 33.30143722879873 35.736532534782214 17.00806882788465 13.953961408534393 7 29.14054979546255 31.719247979182065 20.598938853351104 18.541263372004284 8 26.734580782323718 34.77199095687109 19.30533371768444 19.188094543120748 9 26.596967057596167 16.121323740520165 18.861477013575044 38.420232188308624 10 14.966776093294106 28.946173825739052 33.045380719435244 23.041669361531593 11 35.17783299477655 23.146885277616562 22.771238392338944 18.904043335267946 12 23.759066984749207 24.599384067740456 29.60177815072808 22.039770796782257 13 28.18517246466899 21.832635138240207 25.572473618142105 24.40971877894869 14 22.236748140134928 21.25392063729139 26.63402971067556 29.875301511898115 15 23.900448653344707 28.82695982796281 23.42939589289633 23.843195625796145 16 23.672545103992658 28.271033263986755 24.98075889459018 23.075662737430406 17 22.617901217776083 27.911917521286146 26.265940051201603 23.204241209736164 18 22.532237292100714 27.59173078661018 27.16670600961822 22.709325911670895 19 23.554919106190457 27.16627202707275 27.183839561543365 22.09496930519343 20 23.579753715652977 26.596804984333268 27.301225980323252 22.522215319690506 21 23.840412483216568 26.371328619106976 27.180440546055312 22.60781835162114 22 23.59176368511907 26.135939705966827 27.15529099489387 23.117005614020236 23 23.414484581625675 26.222605632993645 27.075089850560513 23.28781993482017 24 23.043506390636015 26.373874881638095 27.172417170851105 23.410201556874785 25 23.461526249181627 26.183046480579037 27.09523975124476 23.260187518994577 26 23.0195197569547 26.60188603200068 27.294582064826795 23.084012146217823 27 23.196068796068797 26.575301455301453 27.022309582309585 23.206320166320165 28 23.28028616260817 26.489606224018587 27.03113551507425 23.198972098299 29 23.30148436975522 26.59101177008508 26.98724892329819 23.120254936861517 30 23.35515762565649 26.659008560488708 26.99591282095785 22.989920992896952 31 23.447469190999325 26.16260451231317 27.041794643756234 23.348131652931272 32 23.132389651102038 26.630016738587763 27.01343803819881 23.224155572111385 33 23.434510028764205 26.391973359847686 26.811491650155677 23.362024961232432 34 23.50939337739371 26.443122552453264 26.9479356829782 23.099548387174824 35 23.434683785621143 26.516506872016706 26.88094391340848 23.167865428953675 36 23.433015735920566 26.50408171041721 26.86377544756074 23.199127106101486 37 23.348501965267914 26.277484638546472 27.04943643163605 23.32457696454956 38 23.409764331454845 26.43724362074425 26.956391293078447 23.19660075472246 39 23.48195679596914 26.66429614148649 26.76670645762505 23.087040604919324 40 23.59244099689412 26.344543114391307 27.05167605326665 23.011339835447927 41 23.40015387555919 26.422713822912456 26.817715528608517 23.359416772919836 42 23.45602281292656 26.424741277290288 26.851969245880152 23.267266663903005 43 23.286292700898166 26.2894281381141 26.890744864458732 23.533534296528998 44 23.380980577996784 26.508817653573303 26.781630538591482 23.32857122983843 45 23.489560464151403 26.56296683371376 26.533361638198507 23.414111063936335 46 23.508715250394992 26.389054004876943 26.471364955137354 23.630865789590704 47 23.408971512512554 26.54957855963542 26.491630320478563 23.54981960737346 48 23.214607705628858 26.700862003240832 26.645073221263633 23.439457069866677 49 23.48788010633386 26.503194191213357 26.509003461962287 23.499922240490495 50 23.548791113047265 26.55887727195721 26.553099058544493 23.33923255645103 51 23.404934472779313 26.75332023242762 26.313093492964697 23.528651801828378 52 23.60405922455331 26.732243161417884 26.217307548032824 23.446390065995978 53 23.46525297402987 26.763758215485282 26.297326760945104 23.47366204953974 54 23.35966054381341 26.671808109264727 26.537495482335366 23.431035864586498 55 23.414913726616966 26.38332299968243 26.495168388101682 23.70659488559892 56 23.29149030398513 26.844547942818558 26.416800375595034 23.447161377601276 57 23.497362791512177 26.501382043028283 26.333462076007375 23.667793089452164 58 23.39534942819301 26.487209445146643 26.59805547504966 23.519385651610687 59 23.59035492064549 26.631075260846476 26.399403347246153 23.379166471261883 60 23.37392968241534 26.72778055641537 26.623997685914304 23.274292075254987 61 23.634211246027732 26.47457990159181 26.570150322985064 23.32105852939539 62 23.303739304666397 26.814719514327916 26.491446456311234 23.390094724694443 63 23.318330009965564 26.58314920897391 26.551282404348896 23.54723837671163 64 23.506268780092398 26.427241636911507 26.5560453478623 23.510444235133793 65 23.36558654571636 26.47084726649712 26.55440841821857 23.609157769567943 66 23.395626151124322 26.555606111577678 26.439132297311364 23.60963543998664 67 23.434998871324314 26.525573128198797 26.371976404928894 23.667451595547995 68 23.309100666657994 26.542953110077377 26.648410739011126 23.499535484253506 69 23.39479860813021 26.536367181130927 26.345761001449286 23.723073209289577 70 23.18333468463617 26.28524026366137 26.684066203351314 23.84735884835115 71 23.331303493170388 26.364296109815864 26.515006447994498 23.78939394901925 72 23.17068633124472 26.66582368007812 26.404185246907847 23.75930474176931 73 23.256621524457767 26.476297239832867 26.47278838617838 23.79429284953098 74 23.20285903119012 26.656200894430306 26.39138055937435 23.749559515005224 75 23.269401430003448 26.633095584393107 26.39712171791637 23.70038126768707 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1731.0 1 1595.5 2 1600.5 3 1650.0 4 1559.0 5 1351.5 6 1086.5 7 1092.5 8 1156.0 9 1516.0 10 2241.0 11 2763.5 12 2921.0 13 2936.0 14 3131.5 15 3538.5 16 3765.0 17 4041.0 18 4641.5 19 5569.0 20 6172.0 21 7212.5 22 9669.5 23 12651.0 24 14216.0 25 16646.0 26 22417.5 27 28738.5 28 31718.0 29 36067.5 30 44435.0 31 53109.5 32 57766.0 33 63370.0 34 73890.5 35 84399.0 36 89991.0 37 95010.0 38 103354.0 39 110846.0 40 115013.0 41 121226.5 42 131460.5 43 139876.5 44 144272.0 45 148511.5 46 156272.0 47 163230.0 48 166667.0 49 168626.5 50 171443.0 51 170170.0 52 168040.0 53 165180.5 54 159985.5 55 151395.5 56 145141.0 57 137790.5 58 121823.5 59 102636.0 60 92065.0 61 83790.5 62 65721.5 63 48369.0 64 40811.0 65 33943.5 66 22700.0 67 15829.0 68 13334.0 69 11125.0 70 7269.0 71 4454.5 72 3287.0 73 2905.5 74 2202.5 75 1427.5 76 974.0 77 762.0 78 430.0 79 246.0 80 182.0 81 157.0 82 102.0 83 61.5 84 51.0 85 42.5 86 32.0 87 28.0 88 26.0 89 19.5 90 14.0 91 13.5 92 12.0 93 11.0 94 6.5 95 3.5 96 4.0 97 6.0 98 7.5 99 9.5 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1885436916649561 2 0.12868423912387256 3 0.13067653557758485 4 0.15153527572175468 5 0.15216918822975406 6 0.12379405691930595 7 0.12300921286178292 8 0.09919221434694937 9 0.11754549076902643 10 0.12068486699911855 11 0.1388570255617672 12 0.13897777080138615 13 0.15648583054613072 14 0.15884036271869983 15 0.14000410533814703 16 0.14302273632862023 17 0.12889554329320566 18 0.12820125816539685 19 0.16671898960383486 20 0.14474335599318996 21 0.156455644236226 22 0.15002596022651807 23 0.1691037080863087 24 0.15419167099337108 25 0.17794829688839517 26 0.16279476931621972 27 0.17764643378934786 28 0.17595600043468285 29 0.1781596010577283 30 0.16871128605754718 31 0.17668047187239644 32 0.16397203540250424 33 0.17725401176058633 34 0.19283014767142806 35 0.18160084038686775 36 0.1828686654028665 37 0.19907871382170758 38 0.19255847088228548 39 0.16530023303831248 40 0.19473188519542617 41 0.18691363093010058 42 0.2404943310109999 43 0.20170492278341928 44 0.18407611779905575 45 0.1783407189171567 46 0.21139472826283823 47 0.19201511730400028 48 0.2244654004515872 49 0.232374213646627 50 0.2185488837102597 51 0.23077433922167617 52 0.21444354556321618 53 0.2055083978314155 54 0.190022820850288 55 0.19349424648933217 56 0.21347758364626476 57 0.19388666851809366 58 0.21957521824702061 59 0.2310762023207235 60 0.23494004998852921 61 0.22168825994035185 62 0.2358758255955759 63 0.2529914633115589 64 0.23337036187348315 65 0.2593909610113621 66 0.22023931706492472 67 0.2415508518576655 68 0.2163452830872143 69 0.21103249254398143 70 0.22060155278378152 71 0.21374926043540732 72 0.21290404375807487 73 0.2063838008186527 74 0.2036066603074174 75 0.23343073449329257 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 3312760.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.116980420236246 #Duplication Level Percentage of deduplicated Percentage of total 1 78.69668578988936 49.6709717613793 2 11.870805126272282 14.984987494547356 3 4.155429496250108 7.868344864574708 4 1.8657385186887445 4.710391262134324 5 1.0239177640086772 3.2313298731433884 6 0.5919220050324023 2.241619776116227 7 0.38462546562069383 1.699347858789391 8 0.25834278506560304 1.3044653205355976 9 0.19020997114966992 1.0804931122308676 >10 0.9056507827199437 9.827254903810593 >50 0.04192889220808851 1.7990360250026174 >100 0.01421545818532366 1.3836415964649917 >500 5.279449090848942E-4 0.1981161512706866 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.0186309904732006E-5 0.0 10 0.0 0.0 0.0 6.037261980946401E-5 0.0 11 0.0 0.0 0.0 6.037261980946401E-5 0.0 12 0.0 0.0 0.0 6.037261980946401E-5 0.0 13 0.0 0.0 0.0 9.055892971419602E-5 0.0 14 0.0 0.0 0.0 9.055892971419602E-5 0.0 15 0.0 0.0 0.0 9.055892971419602E-5 0.0 16 0.0 0.0 0.0 9.055892971419602E-5 0.0 17 0.0 0.0 0.0 9.055892971419602E-5 0.0 18 0.0 0.0 0.0 9.055892971419602E-5 0.0 19 0.0 0.0 0.0 9.055892971419602E-5 0.0 20 0.0 0.0 0.0 9.055892971419602E-5 0.0 21 0.0 0.0 0.0 1.2074523961892802E-4 3.0186309904732006E-5 22 0.0 0.0 0.0 1.8111785942839204E-4 3.0186309904732006E-5 23 0.0 0.0 0.0 2.1130416933312404E-4 3.0186309904732006E-5 24 0.0 0.0 0.0 2.716767891425881E-4 3.0186309904732006E-5 25 0.0 0.0 0.0 3.622357188567841E-4 3.0186309904732006E-5 26 0.0 0.0 0.0 6.640988179041042E-4 3.0186309904732006E-5 27 0.0 0.0 0.0 9.055892971419602E-4 3.0186309904732006E-5 28 0.0 0.0 0.0 0.0015395018051413323 3.0186309904732006E-5 29 0.0 0.0 0.0 0.0025054637220927566 3.0186309904732006E-5 30 0.0 0.0 0.0 0.003350680399425253 3.0186309904732006E-5 31 0.0 0.0 0.0 0.007425832236564073 3.0186309904732006E-5 32 0.0 0.0 0.0 0.011410425143988698 3.0186309904732006E-5 33 0.0 0.0 0.0 0.016451538898078943 3.0186309904732006E-5 34 0.0 0.0 0.0 0.023575508035595695 3.0186309904732006E-5 35 0.0 0.0 0.0 0.03275214624663423 3.0186309904732006E-5 36 0.0 0.0 0.0 0.04377014936186141 3.0186309904732006E-5 37 0.0 0.0 0.0 0.05898404955384634 3.0186309904732006E-5 38 0.0 0.0 0.0 0.07483186225383064 3.0186309904732006E-5 39 0.0 0.0 0.0 0.09285308926695565 3.0186309904732006E-5 40 0.0 0.0 0.0 0.10824810731836898 3.0186309904732006E-5 41 0.0 0.0 0.0 0.12418647894806747 3.0186309904732006E-5 42 0.0 0.0 0.0 0.1421171470314783 3.0186309904732006E-5 43 0.0 0.0 0.0 0.16101377703184053 3.0186309904732006E-5 44 0.0 0.0 0.0 0.18474021661695988 3.0186309904732006E-5 45 0.0 0.0 0.0 0.2099457853874111 3.0186309904732006E-5 46 0.0 0.0 0.0 0.23789830835919293 3.0186309904732006E-5 47 0.0 0.0 0.0 0.26630362597954577 3.0186309904732006E-5 48 0.0 0.0 0.0 0.2945580120503749 3.0186309904732006E-5 49 0.0 0.0 0.0 0.3235066832490129 3.0186309904732006E-5 50 0.0 0.0 0.0 0.35465895507069634 3.0186309904732006E-5 51 0.0 0.0 0.0 0.3849358239051425 3.0186309904732006E-5 52 0.0 0.0 0.0 0.416088095726826 3.0186309904732006E-5 53 0.0 0.0 0.0 0.4494137818616501 3.0186309904732006E-5 54 0.0 0.0 0.0 0.4867542472138036 3.0186309904732006E-5 55 0.0 0.0 0.0 0.5290754537002379 3.0186309904732006E-5 56 0.0 0.0 0.0 0.5736002608097176 3.0186309904732006E-5 57 0.0 0.0 0.0 0.6204494137818617 3.0186309904732006E-5 58 0.0 0.0 0.0 0.671434091210954 3.0186309904732006E-5 59 0.0 0.0 0.0 0.7225998864994747 3.0186309904732006E-5 60 0.0 0.0 0.0 0.7757579782417078 3.0186309904732006E-5 61 0.0 0.0 0.0 0.8308781801277485 3.0186309904732006E-5 62 0.0 0.0 0.0 0.8859983820137891 3.0186309904732006E-5 63 0.0 0.0 0.0 0.944016469650684 3.0186309904732006E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3265 0.0 34.684254 1 GTATCAA 6325 0.0 31.823631 1 TCAACGC 7850 0.0 25.007795 4 TATCAAC 7900 0.0 24.980534 2 ATCAACG 7915 0.0 24.890358 3 CAACGCA 8110 0.0 24.12135 5 AACGCAG 8480 0.0 23.13101 6 ACGCAGA 9670 0.0 20.00201 7 GTACATG 9310 0.0 19.728937 1 CGCAGAG 10015 0.0 19.21115 8 TACATGG 9350 0.0 18.892574 2 AGAGTAC 9555 0.0 18.584879 11 GTATACG 115 0.0011052996 18.013374 1 ACATGGG 9740 0.0 17.676144 3 CAGAGTA 10450 0.0 17.322863 10 GTGTAGC 1380 0.0 17.26282 1 GCGATAC 320 5.456968E-12 17.240894 14 GAGTACT 5315 0.0 17.061968 12 TAGGTCG 185 5.4507236E-6 16.784567 5 GCAGAGT 11090 0.0 16.540808 9 >>END_MODULE