FastQCFastQC Report
Mon 6 Feb 2017
SRR2077504_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077504_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3312760
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36150.038.2764781
GTATCAA72550.033.471871
TTACGCG605.399582E-428.7725185
TATCAAC98850.024.238192
TCAACGC99300.024.1987174
ATCAACG100300.023.958543
CAACGCA101800.023.5380675
AACGCAG105500.022.7446026
TACGCGA800.002887931721.5787356
CACGCGA800.002888438321.57808310
CGAATCG2950.019.87470237
ACGCAGA123600.019.299817
GAGTACT79400.018.653912
CGCAGAG128300.018.5942088
GTGGTAT18500.018.3246061
AGAGTAC122950.018.22422411
GTACTTT83000.017.5398614
GTACATG90950.017.457831
AGTACTT84850.017.20510713
TACATGG92400.017.024842
Produced by FastQC (version 0.11.5)