##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077504_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3312760 Sequences flagged as poor quality 0 Sequence length 75 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.847427522669918 31.0 31.0 34.0 27.0 34.0 2 30.923274852388946 33.0 31.0 34.0 27.0 34.0 3 31.004198915707747 33.0 31.0 34.0 27.0 34.0 4 34.394515449353406 37.0 35.0 37.0 31.0 37.0 5 34.11421141284005 37.0 35.0 37.0 30.0 37.0 6 34.09561181612915 37.0 35.0 37.0 30.0 37.0 7 33.928940822758065 37.0 35.0 37.0 29.0 37.0 8 33.86814227411584 37.0 35.0 37.0 29.0 37.0 9 35.3369142346563 39.0 35.0 39.0 28.0 39.0 10 35.21847824774508 39.0 35.0 39.0 28.0 39.0 11 35.22610089472222 39.0 35.0 39.0 28.0 39.0 12 35.12097344812181 39.0 35.0 39.0 27.0 39.0 13 35.07048050568106 39.0 35.0 39.0 27.0 39.0 14 36.302269406778635 40.0 36.0 41.0 27.0 41.0 15 36.34590854755551 40.0 36.0 41.0 27.0 41.0 16 36.32338774918799 40.0 36.0 41.0 27.0 41.0 17 36.31567484514423 40.0 36.0 41.0 27.0 41.0 18 36.31422378922711 40.0 36.0 41.0 27.0 41.0 19 36.34543100013282 40.0 37.0 41.0 27.0 41.0 20 36.306694417947575 40.0 37.0 41.0 27.0 41.0 21 36.28329248119393 40.0 37.0 41.0 27.0 41.0 22 36.26593082505222 40.0 37.0 41.0 27.0 41.0 23 36.243192081527184 40.0 36.0 41.0 27.0 41.0 24 36.2257293012473 40.0 36.0 41.0 27.0 41.0 25 36.197360810925026 40.0 36.0 41.0 27.0 41.0 26 36.12992459459786 40.0 36.0 41.0 27.0 41.0 27 36.07615734312175 40.0 36.0 41.0 27.0 41.0 28 36.056082541445804 40.0 36.0 41.0 27.0 41.0 29 35.99875390912713 40.0 36.0 41.0 27.0 41.0 30 35.92528194013451 40.0 36.0 41.0 27.0 41.0 31 35.842454328113114 40.0 36.0 41.0 27.0 41.0 32 35.778369697774664 40.0 36.0 41.0 27.0 41.0 33 35.72083398737005 40.0 35.0 41.0 26.0 41.0 34 35.6643870971637 40.0 35.0 41.0 26.0 41.0 35 35.58712070901605 40.0 35.0 41.0 25.0 41.0 36 35.53676662360086 40.0 35.0 41.0 25.0 41.0 37 35.48347390091646 40.0 35.0 41.0 25.0 41.0 38 35.43055911083206 40.0 35.0 41.0 25.0 41.0 39 35.35470242335696 40.0 35.0 41.0 25.0 41.0 40 35.2945187698475 40.0 35.0 41.0 25.0 41.0 41 35.24685005856144 40.0 35.0 41.0 25.0 41.0 42 35.192580205025415 40.0 35.0 41.0 24.0 41.0 43 35.09620135476159 40.0 35.0 41.0 24.0 41.0 44 34.97724042792113 39.0 35.0 41.0 24.0 41.0 45 34.89130392784264 39.0 35.0 41.0 23.0 41.0 46 34.76684124415895 39.0 35.0 41.0 23.0 41.0 47 34.62133085403108 39.0 34.0 41.0 22.0 41.0 48 34.52560010384091 39.0 34.0 41.0 21.0 41.0 49 34.4338394571294 39.0 34.0 41.0 20.0 41.0 50 34.430528018932854 39.0 34.0 41.0 20.0 41.0 51 34.358667395162946 39.0 34.0 41.0 19.0 41.0 52 34.290936258588005 39.0 34.0 41.0 19.0 41.0 53 34.19335870995786 39.0 34.0 41.0 18.0 41.0 54 34.15858257163212 39.0 34.0 41.0 18.0 41.0 55 34.07473285115734 39.0 34.0 41.0 18.0 41.0 56 33.95071118946136 39.0 34.0 41.0 15.0 41.0 57 33.872697388280464 38.0 34.0 41.0 15.0 41.0 58 33.76307972808172 38.0 34.0 41.0 14.0 41.0 59 33.66690161677876 38.0 34.0 41.0 13.0 41.0 60 33.63111936874389 38.0 34.0 40.0 13.0 41.0 61 33.513452529009044 38.0 34.0 40.0 12.0 41.0 62 33.35854212197684 37.0 33.0 40.0 8.0 41.0 63 33.21817095111025 37.0 33.0 40.0 8.0 41.0 64 33.06181280865502 37.0 33.0 40.0 7.0 41.0 65 32.92698384428694 37.0 33.0 40.0 2.0 41.0 66 32.72579420181359 36.0 33.0 40.0 2.0 41.0 67 32.52846297347227 36.0 33.0 39.0 2.0 41.0 68 32.31316394788635 36.0 33.0 39.0 2.0 41.0 69 32.145717166350714 36.0 33.0 39.0 2.0 41.0 70 31.948337639913547 35.0 32.0 39.0 2.0 41.0 71 31.769535070454847 35.0 32.0 39.0 2.0 41.0 72 31.5673003779326 35.0 32.0 38.0 2.0 40.0 73 31.361696591361884 35.0 32.0 38.0 2.0 40.0 74 31.10131521752255 35.0 32.0 37.0 2.0 40.0 75 30.882724072978423 35.0 32.0 37.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4958320683745221 1101 2 0.44713432091793237 1101 3 0.5729000673363274 1101 4 0.5506254193249518 1101 5 0.5964807200441413 1101 6 0.5771375075025915 1101 7 0.6098536375055232 1101 8 0.6499722137269117 1101 9 0.8380166819016566 1101 10 0.7646536431994946 1101 11 0.8623858671141846 1101 12 0.8089739695270595 1101 13 0.7829005108196654 1101 14 0.9007708464166342 1101 15 0.8853233344836013 1101 16 0.8053210502183177 1101 17 0.8878080140060831 1101 18 0.857684061414119 1101 19 0.8837520005776156 1101 20 0.8779422101215388 1101 21 0.8523057000058003 1101 22 0.9094895897283592 1101 23 0.8551376047574735 1101 24 0.882491303748246 1101 25 0.8869799590820264 1101 26 0.9034746325445724 1101 27 0.8784565650285998 1101 28 0.8042578510005072 1101 29 0.7824943304461698 1101 30 0.7994650297994852 1101 31 1.0032585282320738 1101 32 0.8770615318383364 1101 33 0.8607321915917581 1101 34 0.9097354261717285 1101 35 1.043918138177375 1101 36 0.9974194542557129 1101 37 0.9488790815079682 1101 38 0.9380318571285358 1101 39 1.0213567839638245 1101 40 1.020958145631191 1101 41 1.0343300365229098 1101 42 1.0095708765841778 1101 43 1.059941030685529 1101 44 1.0643666253131272 1101 45 1.021261750557926 1101 46 1.0877403934360643 1101 47 1.0088175497815826 1101 48 1.0499135095153846 1101 49 1.0350057783838977 1101 50 0.97573846087883 1101 51 1.0798835954775257 1101 52 1.046049704886947 1101 53 0.9661414219901445 1101 54 0.987670329718128 1101 55 1.0043691268897632 1101 56 1.02538011874244 1101 57 0.9905029098642331 1101 58 0.9812931440184585 1101 59 1.01265662792526 1101 60 0.9800029442538971 1101 61 0.9567849071427617 1101 62 0.9776152502175819 1101 63 1.0413644308980565 1101 64 0.9880803775944287 1101 65 0.9490737729521541 1101 66 0.9692452821401147 1101 67 0.9433422476444235 1101 68 1.0501205985165925 1101 69 0.9994165578329017 1101 70 0.9955628753116059 1101 71 0.984921071002578 1101 72 0.9612528614065177 1101 73 1.0080039844920279 1101 74 1.093118800365506 1101 75 1.1319466539188028 1102 1 0.6830174440832764 1102 2 0.7421082221434716 1102 3 0.7355327926478026 1102 4 0.7349210597229572 1102 5 0.7502142328594203 1102 6 0.8763478628996353 1102 7 0.853448241721587 1102 8 0.8846363566660855 1102 9 1.027912285077356 1102 10 1.1284141387326443 1102 11 1.1264718161085767 1102 12 1.136066524271989 1102 13 1.1180679835667533 1102 14 1.2634379799159134 1102 15 1.1914511513194768 1102 16 1.3009008583956927 1102 17 1.2601578239064537 1102 18 1.2000146265543634 1102 19 1.2536339768679028 1102 20 1.3185559741562969 1102 21 1.2312712738628946 1102 22 1.3436838010730128 1102 23 1.2214419409487718 1102 24 1.3068904035071327 1102 25 1.3684663081989328 1102 26 1.4180416573809609 1102 27 1.3517491730863682 1102 28 1.3236570854226386 1102 29 1.4043978093504776 1102 30 1.4412478262580493 1102 31 1.4094046281943378 1102 32 1.4259561191696974 1102 33 1.4211770447449297 1102 34 1.463035832613052 1102 35 1.4428632932870045 1102 36 1.5072513824297857 1102 37 1.5147480583054929 1102 38 1.4831317461867286 1102 39 1.5485593223316272 1102 40 1.4573149538772796 1102 41 1.471289031216834 1102 42 1.5573098201267825 1102 43 1.5385511108170675 1102 44 1.5354195072276084 1102 45 1.5521637733726408 1102 46 1.530882719029023 1102 47 1.5671740003068209 1102 48 1.5771371537585495 1102 49 1.533927220675686 1102 50 1.5251076930185974 1102 51 1.5026726492886127 1102 52 1.4910447292523656 1102 53 1.5333703681673114 1102 54 1.5755554105143545 1102 55 1.524584886772061 1102 56 1.5347401644312697 1102 57 1.4788562648610863 1102 58 1.389934916628853 1102 59 1.440709547476395 1102 60 1.4761621065517758 1102 61 1.4859477022408782 1102 62 1.5464223029810569 1102 63 1.4905265060656987 1102 64 1.5181916854059665 1102 65 1.4953185154742883 1102 66 1.5599897990149714 1102 67 1.5214745580894942 1102 68 1.5557475802676493 1102 69 1.5718273632814501 1102 70 1.587185077207728 1102 71 1.512529109777308 1102 72 1.487566967589526 1102 73 1.4978030272089384 1102 74 1.5457222554102508 1102 75 1.5861819082061857 1103 1 0.6978896561366952 1103 2 0.7900059917862094 1103 3 0.8324835078535529 1103 4 0.835502715203944 1103 5 0.8517436206975333 1103 6 0.9810160209906087 1103 7 1.0158507907013146 1103 8 1.0508352584354554 1103 9 1.1725695964460812 1103 10 1.2301143624462938 1103 11 1.1936374319990293 1103 12 1.1974142295123116 1103 13 1.2610196478606994 1103 14 1.336429641498178 1103 15 1.3888669197419574 1103 16 1.3038145591617436 1103 17 1.4000059697948686 1103 18 1.349765829862612 1103 19 1.4104708014899643 1103 20 1.3901565436092156 1103 21 1.4084524819165836 1103 22 1.4214019218783847 1103 23 1.4911131496803876 1103 24 1.3519205709534035 1103 25 1.3669735280484332 1103 26 1.3374179708512344 1103 27 1.3505085792283253 1103 28 1.350531195652458 1103 29 1.3314739397823132 1103 30 1.4015027242310722 1103 31 1.4540009267503677 1103 32 1.4064211723864304 1103 33 1.4022806987344936 1103 34 1.3909146204918343 1103 35 1.4576121238747604 1103 36 1.4677774484592092 1103 37 1.4777580237315675 1103 38 1.4267315021354818 1103 39 1.438273917952266 1103 40 1.3950737498911039 1103 41 1.3852297809965108 1103 42 1.44735388493595 1103 43 1.5136266989127876 1103 44 1.5040480747823537 1103 45 1.5366339787824472 1103 46 1.5058064530131574 1103 47 1.5594077471714414 1103 48 1.5438729682796009 1103 49 1.585302720641792 1103 50 1.5523288983606065 1103 51 1.5511683444957214 1103 52 1.4754023999187567 1103 53 1.527240614252328 1103 54 1.5030762565586926 1103 55 1.5413037532895828 1103 56 1.5989012382567722 1103 57 1.5000575393509479 1103 58 1.5073669551347209 1103 59 1.5249790885244607 1103 60 1.459620855111197 1103 61 1.5286919229716744 1103 62 1.4736489316841954 1103 63 1.5099706115500737 1103 64 1.5156671108008553 1103 65 1.4662235388625362 1103 66 1.5111564995776448 1103 67 1.5206854590453602 1103 68 1.4690375267119578 1103 69 1.4899535920117017 1103 70 1.5405360364647294 1103 71 1.470613307458816 1103 72 1.4863358646134621 1103 73 1.501507182859406 1103 74 1.5429373594709936 1103 75 1.5617618688868191 1104 1 0.5291414040802316 1104 2 0.5824538339524175 1104 3 0.5232628602830438 1104 4 0.5073111606260312 1104 5 0.6489275183812353 1104 6 0.6663507646990183 1104 7 0.6628707723504164 1104 8 0.7154789183587695 1104 9 0.8247684593311817 1104 10 0.9376409572319133 1104 11 0.8944855001457839 1104 12 0.8487700727523233 1104 13 0.8812171805056792 1104 14 0.9780749722351345 1104 15 0.9317538613516234 1104 16 0.9950809718006255 1104 17 1.0094578868231423 1104 18 0.9484523379422143 1104 19 1.082181963086093 1104 20 1.0196777773668657 1104 21 0.9797409316659653 1104 22 1.0309356566878023 1104 23 1.020271532396876 1104 24 1.0645708339963704 1104 25 1.0581417753769387 1104 26 1.0539971702229778 1104 27 1.0596896401348914 1104 28 1.036441548686966 1104 29 1.0652187660697834 1104 30 1.0050195265261337 1104 31 1.0257783599478714 1104 32 1.0371478761555792 1104 33 1.0585857564516914 1104 34 1.1569764285766482 1104 35 1.1352943807954148 1104 36 1.1345247308828093 1104 37 1.1241527925222101 1104 38 1.123884868404275 1104 39 1.1720607349520904 1104 40 1.2254520269096076 1104 41 1.0794041306012403 1104 42 1.0861149185165004 1104 43 1.1137258258425433 1104 44 1.2493252815986295 1104 45 1.1544376401481884 1104 46 1.1367213136384535 1104 47 1.1549556097720597 1104 48 1.166812879089406 1104 49 1.1797363068008835 1104 50 1.0897292405368333 1104 51 1.1830286372286807 1104 52 1.2079999341664305 1104 53 1.2356302624198392 1104 54 1.2328674649536282 1104 55 1.152178546441256 1104 56 1.112461032951039 1104 57 1.143541326047746 1104 58 1.1319749550064984 1104 59 1.1635094135918749 1104 60 1.0882815196703106 1104 61 1.1409306129255583 1104 62 1.1082032010738487 1104 63 1.090889759395047 1104 64 1.1044965395588804 1104 65 1.0516242336325234 1104 66 1.1026640382728061 1104 67 1.0975912083271524 1104 68 1.0837797125529107 1104 69 1.1083304586551321 1104 70 1.056274459969913 1104 71 1.062128218569132 1104 72 1.1091592592099566 1104 73 1.0956655054746918 1104 74 1.12891943328329 1104 75 1.1752044261532575 1105 1 0.3457369091546809 1105 2 0.39536199080074397 1105 3 0.3855103617584561 1105 4 0.5087798770664733 1105 5 0.5045181957160096 1105 6 0.5167341781721859 1105 7 0.48729104692205993 1105 8 0.4894043641265142 1105 9 0.6247321980720955 1105 10 0.5781675775238995 1105 11 0.6498522632382517 1105 12 0.6466036850432317 1105 13 0.6333320400755369 1105 14 0.7212308240554535 1105 15 0.6655695558592214 1105 16 0.6453499126970996 1105 17 0.6642178446408877 1105 18 0.6865591287046868 1105 19 0.6801894514037343 1105 20 0.747102589433311 1105 21 0.7774458300318869 1105 22 0.699406356468181 1105 23 0.8032274635507974 1105 24 0.7106394672332783 1105 25 0.719703757168908 1105 26 0.7577481014252712 1105 27 0.7731870714677882 1105 28 0.7527750980914831 1105 29 0.7927353342144414 1105 30 0.8037870307105024 1105 31 0.7834554722049134 1105 32 0.795474633830139 1105 33 0.7666216694169279 1105 34 0.8003602967667831 1105 35 0.8389099804896887 1105 36 0.9599735558748321 1105 37 0.915014490841692 1105 38 0.8813301717585418 1105 39 0.9022098627662558 1105 40 0.9581602243922163 1105 41 0.8646099238888496 1105 42 0.8698264151157957 1105 43 0.9571398794991381 1105 44 1.0645959929888065 1105 45 0.9527995368016633 1105 46 0.9523785150974149 1105 47 0.9356403167698701 1105 48 0.9322484302160348 1105 49 0.9233800795971945 1105 50 0.8840726776165795 1105 51 0.927560559326956 1105 52 0.8870279234467233 1105 53 0.9570913163957115 1105 54 1.0518212676451668 1105 55 1.0493490846519862 1105 56 0.9892988731422108 1105 57 1.0218855758131298 1105 58 0.9503253192638113 1105 59 0.9272722641834932 1105 60 0.9738302662962184 1105 61 0.9915822900571669 1105 62 0.9781239255250469 1105 63 1.0429738143093772 1105 64 0.9777257459326592 1105 65 0.9293927923732497 1105 66 0.8473466942068626 1105 67 0.8469352742707841 1105 68 1.0255736193456073 1105 69 0.9431430475426197 1105 70 0.908764664205556 1105 71 0.9207685181018341 1105 72 0.9326014472692883 1105 73 0.948013218828855 1105 74 1.0627343538045686 1105 75 1.0082137043142652 1106 1 0.37407984558229046 1106 2 0.37869793754419945 1106 3 0.3461492122381351 1106 4 0.43568619819280485 1106 5 0.4244623922824644 1106 6 0.47526629698256073 1106 7 0.43831387862319815 1106 8 0.412066507217844 1106 9 0.618172557677326 1106 10 0.5547102925243976 1106 11 0.6194641385738322 1106 12 0.5963205339358595 1106 13 0.5580016242582246 1106 14 0.6196930175143294 1106 15 0.6049605267648417 1106 16 0.6274765442965489 1106 17 0.6334798081196737 1106 18 0.6705941008837542 1106 19 0.6281384186315719 1106 20 0.6922775786232975 1106 21 0.7245568334388253 1106 22 0.7538061511966987 1106 23 0.6420504596591883 1106 24 0.686214061085586 1106 25 0.7309510000212285 1106 26 0.7357137279547104 1106 27 0.8443346246031709 1106 28 0.669864918621478 1106 29 0.6152072400556534 1106 30 0.6105772213944078 1106 31 0.7419608296956568 1106 32 0.8107522682663273 1106 33 0.756322932881929 1106 34 0.7300956771129847 1106 35 0.6855086224667559 1106 36 0.7275636211150456 1106 37 0.722100690610759 1106 38 0.6702445992078978 1106 39 0.7315336927900589 1106 40 0.6963074781733454 1106 41 0.5994700209176358 1106 42 0.599296691139422 1106 43 0.7218913441967203 1106 44 0.7927423819687718 1106 45 0.8018526175370297 1106 46 0.8945512947679362 1106 47 0.9030064460243779 1106 48 0.898598009457011 1106 49 0.8777847590268095 1106 50 0.8272460417406364 1106 51 0.8240311632147623 1106 52 0.8245741879333295 1106 53 0.7731265926806756 1106 54 0.8769108030758801 1106 55 0.8650309138490044 1106 56 0.9235258596902796 1106 57 0.920723226024819 1106 58 0.8545007123444535 1106 59 0.8795750776382789 1106 60 0.8877360219756341 1106 61 0.8844040412320098 1106 62 0.9054994060361352 1106 63 0.8855264032484769 1106 64 0.8266634564903654 1106 65 0.9091567871844433 1106 66 0.8462447284472887 1106 67 0.8519877277877228 1106 68 0.9883943916795772 1106 69 0.9721990743039441 1106 70 0.9658628286413204 1106 71 0.9046834064617038 1106 72 0.9234317799326384 1106 73 0.9518023023772031 1106 74 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-1.0321148656700387 2214 25 -0.9382975803466778 2214 26 -0.9273325593937258 2214 27 -0.9486905587997541 2214 28 -1.1057327350038335 2214 29 -0.9468034386365787 2214 30 -0.9540818496163723 2214 31 -0.9429680197462673 2214 32 -0.968592863246748 2214 33 -0.9176141992994502 2214 34 -0.9028251476119848 2214 35 -0.8628422339615227 2214 36 -0.8577113685986859 2214 37 -0.9376215280215945 2214 38 -0.7569412058393681 2214 39 -0.7645744132279262 2214 40 -0.8847461365730425 2214 41 -0.9159607551807625 2214 42 -1.0172280390268327 2214 43 -1.0081932697460232 2214 44 -0.9934302999788827 2214 45 -0.8974561530327918 2214 46 -0.9065618791676613 2214 47 -0.9500199456420617 2214 48 -0.9280066987247793 2214 49 -0.9348706743732791 2214 50 -0.8685567313309619 2214 51 -0.890143625370996 2214 52 -0.9236834881499689 2214 53 -0.952726686564084 2214 54 -1.027750175102696 2214 55 -1.070399945649207 2214 56 -1.1411539751188045 2214 57 -1.1253952549997734 2214 58 -1.1192428185261818 2214 59 -1.0742769067725746 2214 60 -1.0029671691255118 2214 61 -0.895669218962432 2214 62 -0.9181416791830088 2214 63 -1.0197749959489357 2214 64 -1.079160914722376 2214 65 -1.1451717513829252 2214 66 -1.097341392843827 2214 67 -1.085550114450072 2214 68 -0.9582410613980485 2214 69 -1.019789442436796 2214 70 -1.0012650631569961 2214 71 -1.0210821720130632 2214 72 -1.075797908997405 2214 73 -1.0727442291914038 2214 74 -1.0249263832096602 2214 75 -0.9578043219441845 2215 1 -1.2148662113618194 2215 2 -1.2674832387544939 2215 3 -1.0804327874524482 2215 4 -2.1215694773094285 2215 5 -1.4923528588033577 2215 6 -1.4546171489440312 2215 7 -1.307721327539916 2215 8 -1.2317627807802296 2215 9 -1.4565235408088242 2215 10 -1.3415467338103895 2215 11 -1.40436204809604 2215 12 -1.3106246148132996 2215 13 -1.3983401263520108 2215 14 -1.6224495920134103 2215 15 -1.6083605015295817 2215 16 -1.6097824402440821 2215 17 -1.574986602991821 2215 18 -1.6538768469050638 2215 19 -1.4990397451529347 2215 20 -1.5768699715423011 2215 21 -1.5809860207572086 2215 22 -1.6117665273194817 2215 23 -1.6145411878121863 2215 24 -1.6833705758083255 2215 25 -1.6393355182973792 2215 26 -1.6657185413293973 2215 27 -1.5982715009855823 2215 28 -1.4433658631710742 2215 29 -1.6828457274785293 2215 30 -1.5765892246697035 2215 31 -1.4681180572068513 2215 32 -1.5007264063420322 2215 33 -1.5731066304396037 2215 34 -1.690713053304954 2215 35 -1.705986360973725 2215 36 -1.7359699520161342 2215 37 -1.6037437587996308 2215 38 -1.6450532572228056 2215 39 -1.7268215217387635 2215 40 -1.716159538878948 2215 41 -1.5703023788371198 2215 42 -1.6174474371080194 2215 43 -1.6452309956802011 2215 44 -1.5320433542134921 2215 45 -1.5255395951925976 2215 46 -1.5215999640991384 2215 47 -1.6865228826562415 2215 48 -1.7840704363549804 2215 49 -1.8342910107901744 2215 50 -1.7023175662858563 2215 51 -1.6138264564549658 2215 52 -1.6710633606278833 2215 53 -1.631664243145181 2215 54 -1.6149952675054777 2215 55 -1.6590178450467192 2215 56 -1.6424579417788792 2215 57 -1.6661865188784049 2215 58 -1.659976330664449 2215 59 -1.6847071117132089 2215 60 -1.6575657164871416 2215 61 -1.8222498476759768 2215 62 -1.7692157444893013 2215 63 -1.6965007194065507 2215 64 -1.6205936586584428 2215 65 -1.6971810957185731 2215 66 -1.675694733339764 2215 67 -1.6096298261596296 2215 68 -1.5900262730503734 2215 69 -1.589335252313905 2215 70 -1.658566756167474 2215 71 -1.658848590936902 2215 72 -1.7341584164322938 2215 73 -1.679639578875591 2215 74 -1.6508334367262734 2215 75 -1.6582411019985024 2216 1 -1.0984739802269416 2216 2 -0.9449435031632873 2216 3 -1.1816579062878603 2216 4 -1.041669001967776 2216 5 -1.1413876924337814 2216 6 -1.2272668072922173 2216 7 -1.1805128456623208 2216 8 -1.2952253476251485 2216 9 -1.79571323183675 2216 10 -2.0632189708870428 2216 11 -2.136665598304006 2216 12 -1.9217776896796082 2216 13 -1.9557480289069744 2216 14 -2.1023582372896996 2216 15 -1.889718938843906 2216 16 -1.9721450367978548 2216 17 -2.259387969599068 2216 18 -2.0476079075111286 2216 19 -2.3394281884090233 2216 20 -2.2280252745726017 2216 21 -2.237406103942 2216 22 -2.3727394922630296 2216 23 -2.3945434159761803 2216 24 -2.5006046815718435 2216 25 -2.610804249729341 2216 26 -2.5001577867245217 2216 27 -2.402824754105012 2216 28 -2.2959334508121927 2216 29 -2.4240816157732397 2216 30 -2.3445578818295374 2216 31 -2.3815546288051905 2216 32 -2.2947909488256926 2216 33 -2.2367092002554045 2216 34 -2.2294894199122055 2216 35 -2.2987515124888773 2216 36 -2.405353864672101 2216 37 -2.411938945965403 2216 38 -2.4108442554402743 2216 39 -2.292029112350768 2216 40 -2.2637141288967726 2216 41 -2.179618703851979 2216 42 -2.220726923144859 2216 43 -2.2677874425013513 2216 44 -2.375143844409564 2216 45 -2.3492246070761382 2216 46 -2.17560768840098 2216 47 -2.366652858889161 2216 48 -2.3660260216193905 2216 49 -2.387424552085484 2216 50 -2.344438778407067 2216 51 -2.3961043827770077 2216 52 -2.368233963717607 2216 53 -2.3903351433234334 2216 54 -2.471469197986764 2216 55 -2.3714235194376876 2216 56 -2.599482600127068 2216 57 -2.4706495313561234 2216 58 -2.512431024663261 2216 59 -2.561485557940184 2216 60 -2.50563167014133 2216 61 -2.4779747436949897 2216 62 -2.5233207652855008 2216 63 -2.4284132268337615 2216 64 -2.416454101320358 2216 65 -2.3020592894202956 2216 66 -2.4114697630486184 2216 67 -2.4372943389344357 2216 68 -2.472982675308245 2216 69 -2.677723175665065 2216 70 -2.499665983737291 2216 71 -2.4594877026421926 2216 72 -2.5233611050835094 2216 73 -2.545159483807261 2216 74 -2.523931327397694 2216 75 -2.5447027775778253 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 73996.0 3 28570.0 4 16488.0 5 10546.0 6 7911.0 7 7088.0 8 6495.0 9 6864.0 10 7318.0 11 7982.0 12 8495.0 13 9030.0 14 9587.0 15 9978.0 16 10508.0 17 11259.0 18 12012.0 19 13125.0 20 14696.0 21 17220.0 22 21076.0 23 25933.0 24 27371.0 25 28986.0 26 30409.0 27 32797.0 28 38153.0 29 44763.0 30 51937.0 31 59779.0 32 71788.0 33 89186.0 34 116422.0 35 156684.0 36 217962.0 37 339971.0 38 576896.0 39 879231.0 40 214248.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.98278799449023 18.396425895942585 14.73553684057901 25.88524926898818 2 15.238814653526209 19.572291999337 38.245728762008966 26.94316458512783 3 18.15287431754693 23.59683181180687 29.29113026846525 28.959163602180954 4 12.662640437635517 16.277903511367864 35.862044574279025 35.19741147671759 5 14.16719869979463 37.16021514180886 33.916590701501164 14.755995456895347 6 33.404470817168814 36.1793462057441 16.524708872490514 13.891474104596568 7 28.935165544256808 31.708211885295277 20.498295022032007 18.85832754841591 8 26.77018044659642 35.12244051289081 18.86056739738651 19.24681164312626 9 26.312626315706815 15.86368232407612 18.686026719433865 39.1376646407832 10 14.717726777117884 29.190119357728662 32.96117016410064 23.13098370105281 11 35.38607616911239 22.96654675770288 22.488923642193264 19.15845343099146 12 23.64994616736736 24.680758739455193 29.648499953616003 22.02079513956144 13 28.269641901110997 21.664855889095815 25.640398910874694 24.425103298918486 14 22.217195472059153 21.029278437194783 26.797111840090555 29.956414250655506 15 23.88633531071263 28.762384787061368 23.609051341010975 23.742228561215025 16 23.614993788722348 28.149347257224193 25.03317009479672 23.202488859256736 17 22.49406034490307 27.762743500195196 26.44072678613105 23.302469368770684 18 22.4639645813998 27.213254923225282 27.546542163749432 22.776238331625486 19 23.420124517030068 26.75289631007651 27.775261916277362 22.05171725661606 20 23.481501720085678 26.163416441875597 27.8300956877852 22.524986150253522 21 23.648537459898094 25.88672579732025 27.876142668428006 22.58859407435365 22 23.383552466520158 25.703478968920212 27.801982780717566 23.11098578384206 23 23.260192971243306 25.71044894087414 27.735382016220118 23.29397607166244 24 22.919650068310652 26.007533041975194 27.762233645071444 23.31058324464271 25 23.188627197258178 25.856629913234286 27.76859134854735 23.186151540960186 26 22.93773426688299 26.090653602527553 27.904939545442602 23.06667258514685 27 23.07349711653754 26.163251169977087 27.71970794279998 23.0435437706854 28 23.11004774574898 26.065990054655007 27.711196651425833 23.11276554817018 29 23.22171909623364 26.12219528570311 27.64934189087116 23.006743727192088 30 23.242848403116525 26.14500739513196 27.644899593494216 22.967244608257303 31 23.215475615025024 25.715580533323106 27.73943185911299 23.329511992538876 32 23.052223093704015 26.211415042902868 27.628287453741525 23.108074409651593 33 23.21203490385379 25.944789404579723 27.518469799978806 23.324705891587687 34 23.307666413570004 26.080750413986635 27.58852219224981 23.023060980193552 35 23.324859557812008 26.11048873488977 27.506503252909447 23.05814845438878 36 23.317987236072284 26.101930287460075 27.46013536767237 23.119947108795277 37 23.161047060990004 25.89676710409931 27.693772999930566 23.24841283498012 38 23.3284480715794 26.024901250798067 27.60805811599956 23.038592561622973 39 23.41125845887814 26.263669450468768 27.378236834097308 22.94683525655578 40 23.395441946876954 25.885250766583855 27.701836389857213 23.017470896681978 41 23.232511689465955 25.995566939098847 27.4102277996754 23.361693571759794 42 23.355224031990165 26.07231115423373 27.329662812398304 23.242802001377804 43 23.194581094148266 25.886524073308713 27.404998727509906 23.513896105033112 44 23.291900620660485 26.090533289820584 27.299793294306703 23.31777279521223 45 23.355218571127125 26.121036061586985 27.111230118611367 23.412515248674524 46 23.42141884691897 25.930588132303356 27.00793011117853 23.640062909599145 47 23.24057610510205 26.19005068541534 26.973063373372497 23.596309836110112 48 23.138558084957435 26.333103198650708 27.05109925788357 23.477239458508286 49 23.441603525824856 26.168804902345517 26.876958372324687 23.512633199504933 50 23.497170932203797 26.21633010862268 26.91173380923172 23.3747651499418 51 23.29959253854704 26.520433748120116 26.573622893187625 23.606350820145224 52 23.49134196651257 26.43014073945722 26.602269001879463 23.476248292150746 53 23.429594486046224 26.39660778221416 26.674774711954598 23.49902301978502 54 23.24114129642392 26.457855823499386 26.825404443848765 23.47559843622793 55 23.324863013864036 26.154511184685564 26.827940824887165 23.692684976563235 56 23.26547715076486 26.4850433026938 26.701013374808603 23.548466171732734 57 23.41140647267772 26.270767657319805 26.578948718900776 23.738877151101697 58 23.2981058033356 26.21447437929213 26.95702965813901 23.530390159233267 59 23.428862790605706 26.379304463277364 26.727317351346603 23.464515394770327 60 23.271838904196922 26.372953640400976 26.956554025825362 23.398653429576733 61 23.526989730200803 26.169045827254493 26.898294846867664 23.405669595677033 62 23.221517605700782 26.533627901485733 26.768563817211948 23.47629067560154 63 23.132838049937103 26.307771867746112 26.980688294786304 23.578701787530488 64 23.315666267557628 26.19429304264187 26.95142281466668 23.538617875133816 65 23.233946581444958 26.164766337222556 26.948216337147073 23.653070744185413 66 23.28137807976402 26.38254061944752 26.691132181713055 23.644949119075402 67 23.271005915730576 26.30510484616504 26.677547698106324 23.746341539998056 68 23.16077659889873 26.29755696087116 26.979402726779234 23.562263713450875 69 23.18550992043606 26.300506315968697 26.697329796084958 23.816653967510284 70 23.08379726351637 26.075090270146244 26.93818155469948 23.902930911637903 71 23.197537587652196 26.108671212646307 26.75742122924996 23.936369970451533 72 23.096654130310277 26.38503994133263 26.680218450815506 23.838087477541585 73 23.148448353622864 26.18297225155515 26.77704702063005 23.891532374191936 74 23.079426680381832 26.316358352235962 26.659398475128988 23.944816492253217 75 23.141610778571454 26.356883057764296 26.65078043095087 23.85072573271338 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 684.0 1 475.5 2 335.5 3 404.5 4 405.0 5 336.5 6 346.0 7 730.5 8 1037.0 9 1915.5 10 3278.5 11 3814.5 12 3866.0 13 3557.0 14 3400.0 15 3831.0 16 4110.0 17 4500.5 18 5346.5 19 6398.0 20 6994.0 21 7855.5 22 10014.5 23 13106.5 24 14901.0 25 17099.5 26 22674.0 27 28830.5 28 31611.0 29 35968.5 30 44016.0 31 52346.0 32 56986.0 33 62341.0 34 72919.5 35 83806.0 36 89469.0 37 93771.0 38 102313.5 39 110514.0 40 114474.0 41 121146.0 42 131493.5 43 140186.0 44 145203.0 45 150092.5 46 157451.5 47 163839.0 48 167757.0 49 170111.0 50 172745.5 51 171820.5 52 170615.0 53 167202.0 54 161053.0 55 152258.0 56 146199.0 57 139219.5 58 123001.0 59 102994.0 60 92226.0 61 83452.5 62 64542.0 63 46734.5 64 39064.0 65 32471.5 66 21518.0 67 14968.5 68 12780.0 69 10496.5 70 6632.5 71 3947.0 72 2842.0 73 2514.5 74 1873.5 75 1183.5 76 807.0 77 608.5 78 301.0 79 147.0 80 102.0 81 83.0 82 41.5 83 17.0 84 15.0 85 9.5 86 5.5 87 4.0 88 1.0 89 1.5 90 2.0 91 2.0 92 2.0 93 1.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06882478658278898 2 0.016511911517888406 3 0.0023545321725690965 4 0.13943056544995713 5 0.09445296369190644 6 0.06849273717383693 7 0.02979388787597049 8 0.026141344377497915 9 0.048660331566427996 10 0.02870718071940014 11 0.015847812699984302 12 0.10350885666332604 13 0.08898924159914996 14 0.04787548750890496 15 0.019077747859790627 16 0.03145413492073075 17 0.02110023062340767 18 0.018172158562648668 19 0.013342348977891548 20 0.012829181709511103 21 0.026714884265687824 22 0.01639116627826948 23 0.013674398386843598 24 0.020677622284741425 25 0.015304459121699126 26 0.033174754585300475 27 0.02846569024016228 28 0.03806493678986706 29 0.05080355956966397 30 0.03386903971310931 31 0.01989277822721839 32 0.01189340610246441 33 0.013915888866081454 34 0.013734771006653062 35 0.005644839952184885 36 0.009055892971419602 37 0.0075465774761830015 38 0.0022639732428549005 39 0.00796918581484925 40 0.010172786437894685 41 0.011350052524179234 42 0.007305086996945146 43 0.010776512635989326 44 0.009689805479418974 45 0.005342976853137565 46 0.0021130416933312405 47 0.005131672683804441 48 0.0062485661502795255 49 0.0018111785942839206 50 2.4149047923785607E-4 51 0.0016300607348555284 52 0.003441239329139449 53 3.018630990473201E-5 54 3.018630990473201E-5 55 4.8298095847571214E-4 56 0.00796918581484925 57 0.0029280720607590045 58 3.018630990473201E-4 59 0.0072145280672309495 60 0.0014489428754271363 61 0.0011168934664750844 62 0.02393774375445248 63 0.009448315000181118 64 0.011531170383607627 65 0.018564580591410182 66 0.018564580591410182 67 0.017326941885316173 68 0.017326941885316173 69 0.00615800722056533 70 0.014489428754271364 71 0.007123969137516754 72 0.015908185319793766 73 0.00645987031961265 74 0.018443835351791255 75 0.0011772660862845482 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 3312760.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.30958234112176 #Duplication Level Percentage of deduplicated Percentage of total 1 77.18003212830644 47.31875534860826 2 12.718760319401454 15.59563766158671 3 4.454009184159317 8.192203284729846 4 1.9851735260218173 4.868406390201985 5 1.0809769156866371 3.3137121610570865 6 0.6357903331127159 2.3388023867797973 7 0.4184719177755374 1.7959436950214513 8 0.2802414991317299 1.3745191413132958 9 0.21335347688460254 1.1772551302939043 >10 0.9708429577389904 10.314740526378644 >50 0.045995175656315515 1.9186407689995608 >100 0.015857697420315443 1.5161372699263431 >500 3.464080939921574E-4 0.1268826897349659 >1k 1.48460610078816E-4 0.14836354536805754 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 3.0186309904732006E-5 0.0 22 0.0 0.0 0.0 9.055892971419602E-5 0.0 23 0.0 0.0 0.0 1.2074523961892802E-4 0.0 24 0.0 0.0 0.0 1.8111785942839204E-4 0.0 25 0.0 0.0 0.0 2.716767891425881E-4 0.0 26 0.0 0.0 0.0 5.131672683804441E-4 0.0 27 0.0 0.0 0.0 7.546577476183001E-4 0.0 28 3.0186309904732006E-5 0.0 0.0 0.0013583839457129404 0.0 29 3.0186309904732006E-5 0.0 0.0 0.0023847184824738283 0.0 30 3.0186309904732006E-5 0.0 0.0 0.0031997488499015928 0.0 31 3.0186309904732006E-5 0.0 0.0 0.0070635965177072895 0.0 32 3.0186309904732006E-5 0.0 0.0 0.011108562044941378 0.0 33 3.0186309904732006E-5 0.0 0.0 0.016300607348555284 0.0 34 3.0186309904732006E-5 0.0 0.0 0.023666066965309894 0.0 35 3.0186309904732006E-5 0.0 0.0 0.03314456827539574 0.0 36 3.0186309904732006E-5 0.0 0.0 0.044102198770813464 0.0 37 3.0186309904732006E-5 0.0 0.0 0.059044422173655804 0.0 38 3.0186309904732006E-5 0.0 0.0 0.07492242118354484 0.0 39 3.0186309904732006E-5 0.0 0.0 0.09303420712638404 0.0 40 3.0186309904732006E-5 0.0 0.0 0.10876127458674942 0.0 41 3.0186309904732006E-5 0.0 0.0 0.12503169562539998 0.0 42 3.0186309904732006E-5 0.0 0.0 0.14314348156823917 0.0 43 3.0186309904732006E-5 0.0 0.0 0.161858993709173 0.0 44 3.0186309904732006E-5 0.0 0.0 0.18558543329429236 0.0 45 3.0186309904732006E-5 0.0 0.0 0.21100230623407673 0.0 46 3.0186309904732006E-5 0.0 0.0 0.23838128931766864 0.0 47 3.0186309904732006E-5 0.0 0.0 0.26684697955783093 0.0 48 3.0186309904732006E-5 0.0 0.0 0.29537304241780266 0.0 49 3.0186309904732006E-5 0.0 0.0 0.3248046945749164 0.0 50 3.0186309904732006E-5 0.0 0.0 0.3565305062847897 0.0 51 3.0186309904732006E-5 0.0 0.0 0.3870488655984738 0.0 52 3.0186309904732006E-5 0.0 0.0 0.4186841183786329 6.037261980946401E-5 53 3.0186309904732006E-5 0.0 0.0 0.4522512949926949 6.037261980946401E-5 54 3.0186309904732006E-5 0.0 0.0 0.4901049276132288 6.037261980946401E-5 55 3.0186309904732006E-5 0.0 0.0 0.5326374382689962 6.037261980946401E-5 56 3.0186309904732006E-5 0.0 0.0 0.5774339221676186 6.037261980946401E-5 57 3.0186309904732006E-5 0.0 0.0 0.6247056834784288 6.037261980946401E-5 58 3.0186309904732006E-5 0.0 0.0 0.6767166954442821 6.037261980946401E-5 59 3.0186309904732006E-5 0.0 0.0 0.728425844311088 6.037261980946401E-5 60 3.0186309904732006E-5 0.0 0.0 0.7826404568999867 6.037261980946401E-5 61 3.0186309904732006E-5 0.0 0.0 0.8381228945048842 6.037261980946401E-5 62 3.0186309904732006E-5 0.0 0.0 0.8941788719979715 6.037261980946401E-5 63 3.0186309904732006E-5 0.0 0.0 0.9528308721428658 6.037261980946401E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3615 0.0 38.276478 1 GTATCAA 7255 0.0 33.47187 1 TTACGCG 60 5.399582E-4 28.772518 5 TATCAAC 9885 0.0 24.23819 2 TCAACGC 9930 0.0 24.198717 4 ATCAACG 10030 0.0 23.95854 3 CAACGCA 10180 0.0 23.538067 5 AACGCAG 10550 0.0 22.744602 6 TACGCGA 80 0.0028879317 21.578735 6 CACGCGA 80 0.0028884383 21.578083 10 CGAATCG 295 0.0 19.874702 37 ACGCAGA 12360 0.0 19.29981 7 GAGTACT 7940 0.0 18.6539 12 CGCAGAG 12830 0.0 18.594208 8 GTGGTAT 1850 0.0 18.324606 1 AGAGTAC 12295 0.0 18.224224 11 GTACTTT 8300 0.0 17.53986 14 GTACATG 9095 0.0 17.45783 1 AGTACTT 8485 0.0 17.205107 13 TACATGG 9240 0.0 17.02484 2 >>END_MODULE