FastQCFastQC Report
Mon 6 Feb 2017
SRR2077503_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077503_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3798511
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41450.033.4845281
GTATCAA78600.030.2649061
TCAACGC94100.024.783444
ATCAACG94750.024.6855893
CAACGCA96100.024.3766375
TATCAAC98750.023.754912
AACGCAG100150.023.234066
GTACATG105750.020.339971
ACGCAGA116050.019.8735837
TACATGG105900.019.6102032
CGCAGAG119100.019.1935028
CGATTAG2204.2109605E-918.81258418
AGAGTAC112250.018.64737911
GTGTAGC16650.018.4551851
GAGTACT66400.018.43686912
ACATGGG111950.017.9962393
GTGGTAT25050.017.9175361
CGTATAC1753.2135758E-617.7417933
AGTACTT71350.017.4484713
GTACTTT70200.017.04722214
Produced by FastQC (version 0.11.5)