FastQCFastQC Report
Mon 6 Feb 2017
SRR2077503_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077503_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913632
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27300.036.660691
TATGCGA1154.7293724E-1133.01702512
GTATCAA49400.031.8268991
GTATGCG1404.656613E-1027.12112811
CCGTATG1404.656613E-1027.1199
ACCGTAT1555.2750693E-1126.7213618
ATCAACG66150.023.6845043
TCAACGC66150.023.5276534
TATCAAC67250.022.9377022
CAACGCA68800.022.6378785
AACGCAG71150.021.8364986
TACCGTA1605.696893E-821.5719327
GTACCGT2151.382432E-1020.8761436
GTGGTAT14850.019.3560661
CGTGCCG1852.6310045E-718.6582710
ACGCAGA81900.018.5850497
ACGTAAT2053.8360668E-818.50819256
GAGTACT57300.018.4335912
GTATAGG5100.018.3340761
GTACATG51200.018.194821
Produced by FastQC (version 0.11.5)