FastQCFastQC Report
Mon 6 Feb 2017
SRR2077502_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077502_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3207562
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33450.038.396331
GTATCAA65800.034.0535051
TATCAAC86800.025.3569932
TCAACGC86750.025.2932594
ATCAACG86800.025.1590563
CAACGCA87750.025.0443335
CGTGCCG4950.024.37859210
AACGCAG92500.023.8861876
GCGCGAA953.0800267E-421.77610611
ACGCAGA107500.020.3640237
GTGGTAT16250.019.9718111
CGCAGAG110500.019.7509168
GTACATG94900.019.13651
AGAGTAC108000.018.89950811
GAGTACT61500.018.72538212
GTGCCGA6400.018.3168811
ATCGCGC950.007847520518.15384135
TACATGG95700.018.024152
TGGTATC17600.017.8371522
AGTACTT65950.017.620113
Produced by FastQC (version 0.11.5)