FastQCFastQC Report
Mon 6 Feb 2017
SRR2077502_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077502_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3207562
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34040.1061242152139226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40150.044.045771
GTATCAA82300.034.523291
TATCAAC107350.026.1298142
ATCAACG110800.025.3177913
TCAACGC111300.025.1415044
CAACGCA112150.024.9373445
AACGCAG117650.023.7685836
GTGGTAT15400.023.595951
GTATACG1103.1917705E-522.0228841
ACGCAGA136350.020.3809247
CGCAGAG138850.019.9145168
TGGTATC17400.019.4802442
GAGTACT95350.019.4427212
AGAGTAC137750.019.3476911
AGTACTT98700.018.98467613
CCGTTAT4000.018.9693766
CGAATCG3900.018.57025337
GTACATG89500.018.5603891
GCGATAC4100.018.51045414
CAGAGTA147300.018.18705710
Produced by FastQC (version 0.11.5)