FastQCFastQC Report
Mon 6 Feb 2017
SRR2077501_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077501_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3270663
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24750.036.8332941
GTATCAA56150.036.0379181
TCAACGC67050.029.3852654
ATCAACG67650.029.2767663
CAACGCA69450.028.4207845
TATCAAC72700.027.3842242
AACGCAG73250.026.9691226
ACGCAGA85350.022.8643747
CGCAGAG88350.022.012648
AGAGTAC86550.020.8780711
GAGTACT49050.020.17788712
GCAGAGT99350.018.951249
CAGAGTA95750.018.94377310
GTACATG81800.018.911961
GTACTTT53050.018.33312414
AGTACTT54800.018.18821313
GCGCGAC950.00784992918.1528930
ACCGTAC1150.001114592317.9909048
GTGTAGC17100.017.9723871
TACATGG84350.017.4663352
Produced by FastQC (version 0.11.5)