FastQCFastQC Report
Mon 6 Feb 2017
SRR2077501_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077501_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3270663
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34800.041.7466551
GTATCAA72250.035.332161
ATCAACG98950.025.8563863
TATCAAC98350.025.8343962
TCAACGC98850.025.8121954
CAACGCA100150.025.3076325
AACGCAG104600.024.1920286
GTGGTAT16500.023.0600571
TGGTATC16700.021.3209272
ACGCAGA121000.020.7685477
CGCAGAG123500.020.1807378
AGAGTAC122450.019.1985511
CGCTCGA900.005716748519.17818810
GAGTACT89100.019.02321212
CAGAGTA132400.018.1207810
GCAGAGT134500.018.0942389
AGTACTT94800.017.87284713
GTACATG77350.017.842851
GTACTTT95650.017.74493814
CGTGCCG3003.6379788E-1117.2603710
Produced by FastQC (version 0.11.5)