FastQCFastQC Report
Mon 6 Feb 2017
SRR2077500_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077500_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4244030
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68450.034.7035371
GGTATCA33700.033.6049081
TCAACGC88900.026.6233734
CAACGCA88750.026.5131845
ATCAACG89950.026.2742393
TATCAAC90100.026.0770172
AACGCAG93700.025.0176266
ACGCAGA108350.021.2870257
CGCAGAG113550.020.1622838
AGAGTAC107100.019.57577711
GTACATG107450.019.4726471
GCGATAC4500.019.15992714
GAGTACT60500.018.7523512
TACATGG109450.018.6928062
ACCGTAT3150.018.6078918
CAGAGTA120350.017.93504110
GCAGAGT127400.017.3210589
GGTGCGA6000.017.24149311
ACATGGG112650.017.0594393
AGTACTT68500.016.96662513
Produced by FastQC (version 0.11.5)