FastQCFastQC Report
Mon 6 Feb 2017
SRR2077500_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077500_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4244030
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43450.036.8684351
GTATCAA84500.033.6981931
TATCAAC117450.024.1717032
ATCAACG118000.023.798113
TCAACGC118250.023.7188284
CAACGCA120800.023.183355
AACGCAG125500.022.315136
GCGATAC4450.020.15557314
ACGCAGA145200.019.0023617
GTACATG104700.018.3734871
CGCAGAG149700.018.316248
GAGTACT96850.018.07845712
AGAGTAC143200.017.8701511
CGTGCCG5450.017.74248710
TGCGATA5000.017.25807813
TACATGG107700.017.2415262
GTGGTAT20950.017.1756341
GTACTTT101400.017.11243414
AGTACTT104300.016.81132113
ACATGGG109500.016.5495263
Produced by FastQC (version 0.11.5)