FastQCFastQC Report
Mon 6 Feb 2017
SRR2077499_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077499_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3446687
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26550.035.637131
GTATCAA52200.032.8116341
TCAACGC63250.026.7868544
ATCAACG63400.026.559813
CAACGCA65550.025.7958245
TATCAAC66150.025.6114082
AACGCAG69950.024.1979086
ACGCAGA81700.020.2970227
CGCAGAG83500.019.7777678
GTACATG85500.019.1842211
GTATAGA9450.019.0015131
AGAGTAC81150.018.82267211
GAGTACT45050.018.215812
TACATGG87850.017.7925512
CAGAGTA89250.017.49945610
ACATGGG89800.017.3298443
GCAGAGT96050.016.7628469
AGTACTT49950.016.22390213
GTACTTT48900.016.22037314
TATAGCG1503.5210737E-416.1039645
Produced by FastQC (version 0.11.5)