FastQCFastQC Report
Mon 6 Feb 2017
SRR2077499_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077499_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3446687
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33400.036.2625921
GTATCAA61100.032.9624181
TCAACGC81650.024.144014
ATCAACG82050.024.0684593
TATCAAC82950.023.930312
CAACGCA84450.023.3896685
AACGCAG88850.022.4240726
TATCTCG4800.019.3994336
ACGCAGA102400.019.2171527
GTACATG81350.018.9720631
CTCGTAT4800.018.6803939
CGCAGAG105000.018.5771718
TAACACG2053.7225618E-818.5579324
CGCTATC5200.017.90742733
TACATGG84900.017.6373392
GAGTACT68950.017.62626812
AGAGTAC106100.017.50728811
ACATGGG88900.016.4950963
AGTACTT74050.016.40396513
CAGAGTA115000.016.39256110
Produced by FastQC (version 0.11.5)