FastQCFastQC Report
Mon 6 Feb 2017
SRR2077498_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077498_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3438507
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30300.033.1627771
GTATCAA55850.032.8300741
TCAACGC70350.025.6003554
ATCAACG71900.025.095723
CAACGCA72150.025.0094955
TATCAAC73100.024.6830352
AACGCAG75050.024.1634086
ACGCAGA85350.021.1271217
GTACATG87100.020.3772471
CGCAGAG89400.020.0563668
AGAGTAC83250.019.55130811
GAGTACT44900.019.43115212
TACATGG91050.019.0590952
GCGATAC4900.019.00389914
CGAATCG3900.018.58104937
ACATGGG93700.018.0419463
ATTAGCG1753.2110056E-617.7431763
CAGAGTA96150.017.46591810
TGCGATA5000.017.24377413
CGTGCCG6400.017.24176610
Produced by FastQC (version 0.11.5)