FastQCFastQC Report
Mon 6 Feb 2017
SRR2077498_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077498_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3438507
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34500.035.3072361
GTATCAA63900.031.1933291
TATCAAC84800.023.4864962
TCAACGC86150.022.961264
ATCAACG86200.022.9482753
CAACGCA86450.022.809975
AACGCAG90200.022.0093576
CGTGCCG5350.020.65346310
CGAATCG6350.020.63807937
CCGTTAT6950.019.3537266
TACCGAT6500.019.120977
GTACCGA7300.018.9183466
GTACATG79900.018.8400731
GCGATAC4950.018.8189314
ACGCAGA105800.018.534617
TACATGG80450.018.3954052
CGCCAAT7700.017.91340442
CGTTATG7350.017.83095667
CGCAGAG109850.017.7587938
AGAGTAC104250.017.6541911
Produced by FastQC (version 0.11.5)