FastQCFastQC Report
Mon 6 Feb 2017
SRR2077497_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077497_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3305674
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28750.039.982071
GTATCAA57250.035.4536321
TCAACGC74950.026.468614
TATCAAC76000.026.4186972
ATCAACG75750.026.3700583
CAACGCA76950.025.8251115
AACGCAG80050.024.8488986
CGAATCG3100.023.37465737
GTACATG94100.021.1663071
ACGCAGA93950.020.9542167
TACATGG95350.020.2613962
CGCAGAG97400.019.9663358
AGAGTAC94450.019.3866911
GAGTACT53200.019.25140812
CGTTATG4000.018.98173567
CCGTTAT4200.018.89956566
ACATGGG102000.018.568953
TACCGAT4500.018.3878467
CGTGCCG4150.018.27956810
GTGTAGC13900.018.1287121
Produced by FastQC (version 0.11.5)