FastQCFastQC Report
Mon 6 Feb 2017
SRR2077497_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077497_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3305674
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33550.038.567391
GTATCAA70750.035.452981
TATCAAC95100.026.2850632
ATCAACG96350.025.9140643
TCAACGC97000.025.7756734
CAACGCA98750.025.422715
AACGCAG103450.024.1950656
GTGGTAT19150.021.3183881
TGGTATC18100.021.01192
GTACATG92600.020.8106371
ACGCAGA121850.020.3991997
TACATGG93350.019.7407722
CGCAGAG125750.019.4376878
CGTGCCG3950.019.22964110
AGAGTAC120800.019.20648611
GAGTACT80050.019.14993112
TACACCG3550.018.4820375
CAGAGTA129150.018.23205410
AGTACTT84200.018.11803413
GCAGAGT134250.017.9501069
Produced by FastQC (version 0.11.5)