FastQCFastQC Report
Mon 6 Feb 2017
SRR2077496_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077496_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3499697
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27000.032.6102141
GTATCAA50200.032.2586671
TCAACGC61550.025.559554
CAACGCA61600.025.3711455
ATCAACG62850.025.0857643
TATCAAC64750.024.8281232
AACGCAG65850.023.872396
GCGATAC2700.020.43495214
ACGCAGA77700.019.966567
GTACATG82550.019.5333651
CGCAGAG79850.019.2581798
GAGTACT41850.018.53844812
TACATGG84550.018.5242212
TGCGATA2902.1827873E-1117.83603113
AGAGTAC78850.017.75509811
GTATAGC9350.017.7258431
ACATGGG88300.017.5429273
GTATAGG8200.016.8431531
CAGAGTA87700.016.3944910
GCAGAGT91800.016.2632269
Produced by FastQC (version 0.11.5)