FastQCFastQC Report
Mon 6 Feb 2017
SRR2077496_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077496_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3499697
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35550.037.858771
GTATCAA64550.031.9452691
TATCAAC86250.023.7737082
ATCAACG88300.023.0684363
TCAACGC88000.023.029184
CAACGCA88650.022.786055
AACGCAG93400.021.629096
ACGCAGA108000.018.4794837
CGTTATG2801.2732926E-1118.4771367
GTGGTAT18800.018.4034251
GTACATG78100.017.9858721
CGCAGAG110650.017.9744998
CGTATAC1954.485155E-717.734913
GAGTACT75400.017.58588612
AGAGTAC109500.017.06033311
TACATGG80550.016.6558782
AGTACTT80600.016.52895513
GTACTTT80800.016.09531814
CAGAGTA118750.016.05128710
TCCGTTA3502.1827873E-1115.76670365
Produced by FastQC (version 0.11.5)