FastQCFastQC Report
Mon 6 Feb 2017
SRR2077495_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077495_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4304640
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67200.034.8281171
GGTATCA32900.034.414981
ATCAACG88750.025.9283073
TCAACGC88750.025.734844
TATCAAC91900.025.113492
CAACGCA91000.024.8710615
AACGCAG95950.023.6046946
ACGCAGA111450.020.1995377
GTACATG115900.019.866071
CGCAGAG116500.019.2073548
TACATGG115600.019.1590352
GAGTACT61100.018.73508812
AGAGTAC111000.018.66875811
ACATGGG120450.017.8442343
CAGAGTA122200.017.18283710
AGTACTT68350.016.95118513
GCAGAGT128350.016.9504899
TCGATCG2451.5850674E-816.90219341
GTACTTT68650.016.6259614
GTGGTAT21250.016.4071081
Produced by FastQC (version 0.11.5)