FastQCFastQC Report
Mon 6 Feb 2017
SRR2077495_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077495_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4304640
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43800.036.4107481
GTATCAA84100.033.8126031
TCAACGC117000.023.9667994
ATCAACG118050.023.7829173
TATCAAC118250.023.7675022
CAACGCA119550.023.3934215
AACGCAG126400.022.1251456
TCGATCG2352.1827873E-1120.54582841
ACGCAGA146450.018.954617
GTACATG104700.018.6682911
CGCAGAG149950.018.3983488
AGAGTAC147100.017.62954711
TACATGG105950.017.619232
GAGTACT100000.017.54193912
AGTACTT107150.016.62224213
CAGAGTA160800.016.47112310
GTACTTT107450.016.407214
ACATGGG110400.016.1918533
GCAGAGT169750.015.8458779
CCTATAC9650.015.7652343
Produced by FastQC (version 0.11.5)