FastQCFastQC Report
Mon 6 Feb 2017
SRR2077494_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077494_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3901023
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36550.032.7753331
GTATCAA67950.031.9062121
TCAACGC85900.024.859684
ATCAACG86400.024.7560623
TATCAAC88050.024.4087522
CAACGCA87950.024.2020955
AACGCAG92450.022.966686
ACGCAGA104900.020.0452847
GTACATG104950.019.7037751
CGCAGAG110600.018.8268958
TACATGG105600.018.8167862
AGAGTAC104600.018.65561311
GAGTACT59600.018.45309312
GTACTTT62400.017.46223314
ACATGGG112350.017.0728243
AGTACTT64850.017.06772213
CAGAGTA117300.016.87069910
GCAGAGT123900.016.2500679
GTATATG11950.016.1780131
CGAATCG3101.0786607E-915.58629937
Produced by FastQC (version 0.11.5)