FastQCFastQC Report
Mon 6 Feb 2017
SRR2077494_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077494_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3901023
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA84850.033.5992621
GGTATCA44500.032.188051
TATCAAC115400.024.7217432
ATCAACG116900.024.2902623
TCAACGC117750.024.1152294
CAACGCA119000.023.7970545
AACGCAG123400.023.0256966
ACGCAGA145200.019.3304047
GTACATG101700.019.2892991
CGCAGAG149050.018.6925988
TACATGG101750.018.4202632
GAGTACT96500.018.38995412
AGAGTAC141850.018.37648411
GGACCGT5200.017.9262126
GTACTTT102450.017.44239214
AGTACTT103450.017.2479613
CAGAGTA156500.017.05336210
GTGGTAT25800.016.8968281
ACATGGG107150.016.8815783
GCAGAGT161500.016.867239
Produced by FastQC (version 0.11.5)