FastQCFastQC Report
Mon 6 Feb 2017
SRR2077493_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077493_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3605995
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28850.034.3425641
GTATCAA59200.033.122511
TCAACGC75200.025.9219474
CAACGCA75750.025.6889045
ATCAACG76150.025.6884613
TATCAAC76550.025.5077442
AACGCAG79250.024.534266
GTACATG88000.020.7524621
ACGCAGA94150.020.6526647
CGCAGAG96000.020.1124158
TACATGG89000.019.5750182
TATACCG2153.1504896E-919.2571455
GCGATAC4150.019.114114
AGAGTAC92550.019.03935411
GTGTAGC15250.018.562681
GTGGTAT18200.018.3991171
GAGTACT54600.018.12629512
GCACCGT8200.018.077896
CAGAGTA100600.018.0297410
ACATGGG94300.017.7807273
Produced by FastQC (version 0.11.5)