FastQCFastQC Report
Mon 6 Feb 2017
SRR2077493_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077493_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3605995
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32500.039.36781
GTATCAA70450.034.8988761
TATCAAC96150.025.628762
ATCAACG99650.024.525623
TCAACGC99900.024.4995294
CAACGCA100350.024.3281615
AACGCAG104900.023.1728976
GCACCGT7350.020.2005986
ACGCAGA122850.019.5891957
CGAATCG3550.019.43079437
CGCAGAG125450.019.10098
GTACATG90350.018.6773171
CGTACAC4450.018.6435723
GCGCGTT1501.700138E-518.3942336
GAGTACT86350.018.3538612
AGAGTAC125450.018.16562711
TACATGG93100.017.8930642
AGTACTT92350.017.4889213
GTATACG1602.8951566E-517.2899131
GTACTTT93250.016.98021314
Produced by FastQC (version 0.11.5)