##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077493_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3605995 Sequences flagged as poor quality 0 Sequence length 75 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.95838430169759 31.0 31.0 34.0 28.0 34.0 2 31.073173978333305 33.0 31.0 34.0 27.0 34.0 3 31.14849160911205 33.0 31.0 34.0 27.0 34.0 4 34.51215212444831 37.0 35.0 37.0 31.0 37.0 5 34.24098369520756 37.0 35.0 37.0 30.0 37.0 6 34.22279232223006 37.0 35.0 37.0 30.0 37.0 7 34.072687011490586 37.0 35.0 37.0 30.0 37.0 8 34.02134306897264 37.0 35.0 37.0 30.0 37.0 9 35.51256005623968 39.0 37.0 39.0 30.0 39.0 10 35.402674712527336 39.0 35.0 39.0 29.0 39.0 11 35.39946699870632 39.0 37.0 39.0 29.0 39.0 12 35.29571588424277 39.0 35.0 39.0 28.0 39.0 13 35.239782639742984 39.0 35.0 39.0 28.0 39.0 14 36.48968315263887 40.0 37.0 41.0 28.0 41.0 15 36.53171094247219 40.0 37.0 41.0 29.0 41.0 16 36.51128162961957 40.0 37.0 41.0 29.0 41.0 17 36.500263588829156 40.0 37.0 41.0 28.0 41.0 18 36.503066144018504 40.0 37.0 41.0 29.0 41.0 19 36.537440290405286 40.0 37.0 41.0 28.0 41.0 20 36.501072796828616 40.0 37.0 41.0 28.0 41.0 21 36.47763155522956 40.0 37.0 41.0 28.0 41.0 22 36.460595480581645 40.0 37.0 41.0 28.0 41.0 23 36.43439882750808 40.0 37.0 41.0 28.0 41.0 24 36.41510956060671 40.0 37.0 41.0 28.0 41.0 25 36.382164978043505 40.0 37.0 41.0 28.0 41.0 26 36.31110303813511 40.0 37.0 41.0 28.0 41.0 27 36.25824994211029 40.0 36.0 41.0 28.0 41.0 28 36.233823119555076 40.0 36.0 41.0 28.0 41.0 29 36.180859374458365 40.0 36.0 41.0 27.0 41.0 30 36.11102344845182 40.0 36.0 41.0 27.0 41.0 31 36.0272701431921 40.0 36.0 41.0 27.0 41.0 32 35.95944974965301 40.0 36.0 41.0 27.0 41.0 33 35.90562632505037 40.0 36.0 41.0 27.0 41.0 34 35.84474964607549 40.0 35.0 41.0 27.0 41.0 35 35.76725231177525 40.0 35.0 41.0 27.0 41.0 36 35.71272755508535 40.0 35.0 41.0 26.0 41.0 37 35.65804528292468 40.0 35.0 41.0 26.0 41.0 38 35.604537721211486 40.0 35.0 41.0 26.0 41.0 39 35.53103262761041 40.0 35.0 41.0 25.0 41.0 40 35.468623223271244 40.0 35.0 41.0 25.0 41.0 41 35.4210674168988 40.0 35.0 41.0 25.0 41.0 42 35.36313056451825 40.0 35.0 41.0 25.0 41.0 43 35.270561661899144 40.0 35.0 41.0 24.0 41.0 44 35.14766853531411 40.0 35.0 41.0 24.0 41.0 45 35.060462368916205 40.0 35.0 41.0 24.0 41.0 46 34.93385015786212 39.0 35.0 41.0 23.0 41.0 47 34.79258540291931 39.0 35.0 41.0 23.0 41.0 48 34.69743857104627 39.0 35.0 41.0 22.0 41.0 49 34.61166196847195 39.0 35.0 41.0 22.0 41.0 50 34.61080533944168 39.0 35.0 41.0 22.0 41.0 51 34.53792115629667 39.0 34.0 41.0 21.0 41.0 52 34.46556276423012 39.0 34.0 41.0 20.0 41.0 53 34.37147722057296 39.0 34.0 41.0 20.0 41.0 54 34.33714328500178 39.0 34.0 41.0 20.0 41.0 55 34.254740508514296 39.0 34.0 41.0 19.0 41.0 56 34.13113828499485 39.0 34.0 41.0 18.0 41.0 57 34.05264705025936 39.0 34.0 41.0 18.0 41.0 58 33.94533658532527 38.0 34.0 41.0 18.0 41.0 59 33.84881787135035 38.0 34.0 41.0 17.0 41.0 60 33.811484486251366 38.0 34.0 41.0 17.0 41.0 61 33.697567245656195 38.0 34.0 41.0 14.0 41.0 62 33.5463471247187 38.0 34.0 40.0 13.0 41.0 63 33.40761232336706 37.0 34.0 40.0 13.0 41.0 64 33.25212458697253 37.0 34.0 40.0 8.0 41.0 65 33.116311032045246 37.0 33.0 40.0 8.0 41.0 66 32.91609833069652 37.0 33.0 40.0 6.0 41.0 67 32.72181325819919 36.0 33.0 39.0 2.0 41.0 68 32.50877857567745 36.0 33.0 39.0 2.0 41.0 69 32.33989425942077 36.0 33.0 39.0 2.0 41.0 70 32.14593198271212 36.0 33.0 39.0 2.0 41.0 71 31.963691297408896 35.0 33.0 39.0 2.0 41.0 72 31.76544809407667 35.0 33.0 39.0 2.0 40.0 73 31.568580655269905 35.0 33.0 38.0 2.0 40.0 74 31.309715071706975 35.0 32.0 37.0 2.0 40.0 75 31.0901188160272 35.0 32.0 37.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5418670748952685 1101 2 0.5302433111340505 1101 3 0.5305626126301561 1101 4 0.601209723279446 1101 5 0.6301433460989045 1101 6 0.6181178858230965 1101 7 0.6625028951857175 1101 8 0.596704337683633 1101 9 0.796821138888518 1101 10 0.7937174502776188 1101 11 0.7683091704015723 1101 12 0.8058280755378533 1101 13 0.7860590764766897 1101 14 0.8840511102458564 1101 15 0.8718942880864589 1101 16 0.8093454462794085 1101 17 0.9062034392317599 1101 18 0.9031509443852386 1101 19 0.8782327032868196 1101 20 0.9513909992128404 1101 21 0.8427808188127628 1101 22 0.9421408777876863 1101 23 0.8890054639228779 1101 24 0.9360057167360623 1101 25 1.0093004784457804 1101 26 0.9686870148871449 1101 27 0.9786843284557705 1101 28 1.0021299917514312 1101 29 1.0261593056206735 1101 30 0.9936475385405501 1101 31 0.9986974771237982 1101 32 1.0031263327185655 1101 33 0.9704922607271413 1101 34 0.9688635459252168 1101 35 1.0776762336491785 1101 36 0.9967283452448825 1101 37 1.0385595024562804 1101 38 1.0061871499236332 1101 39 0.9869290013713581 1101 40 0.968082008847631 1101 41 0.9649122521995679 1101 42 1.0042375571046733 1101 43 1.056460751041385 1101 44 1.0435591348869764 1101 45 1.0304031770393465 1101 46 1.0729360007341882 1101 47 1.0174061046976917 1101 48 1.0624212181614112 1101 49 0.9905867031967119 1101 50 1.0371584438667654 1101 51 0.9823472427876538 1101 52 0.9583225685939141 1101 53 1.0596728374512026 1101 54 1.0001781904186302 1101 55 0.9911119402401098 1101 56 0.9729984566891972 1101 57 0.938868915175803 1101 58 0.942248569053362 1101 59 1.0066715536746997 1101 60 0.8736923564924126 1101 61 0.8914694003515251 1101 62 0.8686240712130768 1101 63 0.8824627322166663 1101 64 0.8754436026923429 1101 65 0.8501815336930179 1101 66 0.8968209770021005 1101 67 0.8940540721600385 1101 68 0.9638632109351732 1101 69 0.9525791075549108 1101 70 0.8740569089764421 1101 71 0.9156980864669677 1101 72 0.9439911307571407 1101 73 0.8895285598121134 1101 74 0.9641547768579422 1101 75 0.9277060565442099 1102 1 0.6089006209405845 1102 2 0.651756373943627 1102 3 0.6342528329370012 1102 4 0.6101152701595183 1102 5 0.660964280526187 1102 6 0.7725475401272988 1102 7 0.8065171896219496 1102 8 0.7834591637285584 1102 9 0.9362848864506503 1102 10 0.9523306525985333 1102 11 0.9758680325975817 1102 12 0.9283439632230639 1102 13 0.9821242831928245 1102 14 1.1502234535465021 1102 15 1.1035334925651625 1102 16 1.1231621268653456 1102 17 1.1349134233132077 1102 18 1.1587960160665105 1102 19 1.1911076922848238 1102 20 1.223709328804496 1102 21 1.23114008500621 1102 22 1.1796725745012608 1102 23 1.2041905743232277 1102 24 1.226751052983083 1102 25 1.229542606091293 1102 26 1.190342376873872 1102 27 1.280337874365678 1102 28 1.267090422384662 1102 29 1.235025862605859 1102 30 1.169492561872758 1102 31 1.2594655979661908 1102 32 1.2500315347033464 1102 33 1.2943156838965422 1102 34 1.3286231865650322 1102 35 1.3090440222610198 1102 36 1.3288549433227885 1102 37 1.4230864881232108 1102 38 1.3699893536691334 1102 39 1.3819085844463146 1102 40 1.384527341151852 1102 41 1.3133383388686468 1102 42 1.3363004331888888 1102 43 1.3929433447814148 1102 44 1.4491912431251563 1102 45 1.4484706323453338 1102 46 1.467344276309511 1102 47 1.4877403305372 1102 48 1.5012682164771576 1102 49 1.4832813748830773 1102 50 1.440936441219911 1102 51 1.4773908664662656 1102 52 1.403056881732553 1102 53 1.3970867679552015 1102 54 1.4915956343940593 1102 55 1.5142947980679793 1102 56 1.4653930376115483 1102 57 1.4624054300686282 1102 58 1.4228792734163704 1102 59 1.4775870435753617 1102 60 1.4102880965974833 1102 61 1.5290523534437739 1102 62 1.5605447596336361 1102 63 1.4786652200029522 1102 64 1.4907808095263633 1102 65 1.4399897185441546 1102 66 1.4768556019404215 1102 67 1.5352273613340301 1102 68 1.5884671887009745 1102 69 1.5330707386667015 1102 70 1.538446111762532 1102 71 1.4519445503941455 1102 72 1.4941112666283658 1102 73 1.5613268382828025 1102 74 1.542649918255055 1102 75 1.5568794986509822 1103 1 0.4564546596209027 1103 2 0.44473528636363113 1103 3 0.5420336208404493 1103 4 0.4854832366383661 1103 5 0.5251811366596897 1103 6 0.5585728178511076 1103 7 0.6192431781671743 1103 8 0.6183981689696054 1103 9 0.7803996834382474 1103 10 0.8216632967393593 1103 11 0.7630010284221029 1103 12 0.8388656355169104 1103 13 0.7502042832905857 1103 14 0.9060886162093809 1103 15 0.8315786175668407 1103 16 0.8617473759461163 1103 17 0.9256103020287654 1103 18 0.8635161293678024 1103 19 0.9291161703302535 1103 20 1.0121718307443786 1103 21 0.9654997530695937 1103 22 0.9430553121840859 1103 23 0.9806887431973834 1103 24 1.0161795242099032 1103 25 0.98006958174593 1103 26 1.0098399262079525 1103 27 1.0049620872626193 1103 28 0.9251079674662819 1103 29 0.9617292617668554 1103 30 0.9359729999254753 1103 31 1.0178969029473137 1103 32 0.9406335883503871 1103 33 0.9301324320076745 1103 34 0.9777905955800179 1103 35 1.0439083649803251 1103 36 1.0195767502780129 1103 37 1.0282732727346655 1103 38 0.9969113637161868 1103 39 0.9340855896115912 1103 40 1.049105045774212 1103 41 0.976642748112134 1103 42 1.0057721378039162 1103 43 0.9370913794218936 1103 44 1.0376565556018562 1103 45 0.9997727051773424 1103 46 0.9811006320941402 1103 47 1.0696968063195342 1103 48 1.0716385596925875 1103 49 1.0495336706639762 1103 50 1.125038822966573 1103 51 1.0553820413806392 1103 52 1.0213151065241775 1103 53 0.9632895738485274 1103 54 1.0050263344987798 1103 55 1.0436905693098382 1103 56 1.0689295483178611 1103 57 0.9871418200440516 1103 58 1.0588554889429957 1103 59 1.0798374281912189 1103 60 1.024752096675691 1103 61 1.0907100325024643 1103 62 1.0339528043676083 1103 63 0.9800906509621186 1103 64 1.019511827710538 1103 65 1.0365145508589748 1103 66 0.9939846019147609 1103 67 0.9868897938658705 1103 68 0.9982703411457123 1103 69 1.00887751548197 1103 70 1.0398648218727615 1103 71 0.9919610959022336 1103 72 0.9892288753747565 1103 73 0.981268085843439 1103 74 0.9948677373029149 1103 75 1.0951767288058498 1104 1 0.4863527354893087 1104 2 0.5125203124769406 1104 3 0.4433448500757322 1104 4 0.521570570723469 1104 5 0.48901515326809175 1104 6 0.5444920966881313 1104 7 0.5141976482731607 1104 8 0.5774986660111097 1104 9 0.7332743771836903 1104 10 0.7314847915569231 1104 11 0.8051193429428309 1104 12 0.7295406989393669 1104 13 0.7530511670834557 1104 14 0.8195983212969082 1104 15 0.8003975472483305 1104 16 0.8129429543768651 1104 17 0.79698886865242 1104 18 0.8549926608095717 1104 19 0.8338495603942633 1104 20 0.8978651081143596 1104 21 0.8731194147518622 1104 22 0.890157968070092 1104 23 0.8719342194014033 1104 24 0.9017200197744444 1104 25 0.8856969838465787 1104 26 0.98415255732764 1104 27 0.9620562689227441 1104 28 0.9372471878570892 1104 29 0.9548824132240341 1104 30 0.9360547446665279 1104 31 0.9502386948924837 1104 32 0.9707913016884504 1104 33 0.925387699387052 1104 34 0.9436231258112784 1104 35 0.9789470384868082 1104 36 1.0497411914483123 1104 37 1.0475433951676365 1104 38 1.090565757627104 1104 39 1.053403978713341 1104 40 1.0962418493571064 1104 41 1.0111793958304247 1104 42 1.0245659423485094 1104 43 1.059158230132148 1104 44 1.1811410654314258 1104 45 1.093210134394937 1104 46 1.1464994603584557 1104 47 1.1034851801137293 1104 48 1.1488354124947833 1104 49 1.2295357168093872 1104 50 1.060750848246549 1104 51 1.117397493347724 1104 52 1.110948404606404 1104 53 1.11913841796747 1104 54 1.161515586123464 1104 55 1.1085978520429407 1104 56 1.154002671168172 1104 57 1.1253305151443485 1104 58 1.1008388046139288 1104 59 1.107893309881689 1104 60 1.055482307475728 1104 61 1.1707043369028867 1104 62 1.10973153752564 1104 63 1.108458258705113 1104 64 1.0765951469222657 1104 65 1.0251171292485637 1104 66 1.0672457676130875 1104 67 1.064783403717108 1104 68 1.105733816044534 1104 69 1.0843145821546898 1104 70 1.0811039382222205 1104 71 1.0853792260329769 1104 72 1.043089152323283 1104 73 1.0553241780866287 1104 74 1.1687486247917995 1104 75 1.2199358810977223 1105 1 0.43960421430554675 1105 2 0.4497732415093658 1105 3 0.4312207318697183 1105 4 0.43352787474675836 1105 5 0.4252610286858953 1105 6 0.49117662256684724 1105 7 0.545956946546454 1105 8 0.5831249199129758 1105 9 0.7169515501148993 1105 10 0.7023736246588115 1105 11 0.7096870815677221 1105 12 0.7116880301889168 1105 13 0.7879705100207488 1105 14 0.8414461193754974 1105 15 0.7825419601251937 1105 16 0.7927972434078256 1105 17 0.7837997901737523 1105 18 0.8096621001146076 1105 19 0.7756633836996443 1105 20 0.7937453427687231 1105 21 0.7638566473014379 1105 22 0.7718538362810534 1105 23 0.8552294848670314 1105 24 0.8460155964922009 1105 25 0.8441078076546447 1105 26 0.8728360849385766 1105 27 0.9142463202332678 1105 28 0.8829704939322056 1105 29 0.8262394086002232 1105 30 0.884326404490686 1105 31 0.8760493604571735 1105 32 0.838428815716604 1105 33 0.7687936578070591 1105 34 0.8413464817752114 1105 35 0.8528614499272251 1105 36 0.9050597935736064 1105 37 0.8247624735344772 1105 38 0.8416384899870124 1105 39 0.8228150584780565 1105 40 0.9227766031207949 1105 41 0.8423578027724901 1105 42 0.9316794542665932 1105 43 0.9598311392901522 1105 44 0.9514980358632101 1105 45 0.9699442775244691 1105 46 0.9311047556750367 1105 47 1.0271337001547138 1105 48 0.9501470397707905 1105 49 1.066503806523727 1105 50 0.9219129540803763 1105 51 0.976403259683515 1105 52 0.9033933398289804 1105 53 0.9330660167660554 1105 54 0.9909144615608056 1105 55 0.9743320955644919 1105 56 0.9486076629739983 1105 57 0.9411134309783549 1105 58 0.8753491723325695 1105 59 0.9049800243801727 1105 60 0.9329947959604468 1105 61 0.9430592559951521 1105 62 0.9433849088236244 1105 63 0.966196191787013 1105 64 0.9562494752007353 1105 65 0.8658161894834038 1105 66 0.8956528571329088 1105 67 0.9525941535766904 1105 68 0.9740400982134503 1105 69 0.9258246735188038 1105 70 0.9175431880861638 1105 71 0.8471544604643171 1105 72 0.8852058044103757 1105 73 0.9217098638310972 1105 74 0.9840275837425203 1105 75 0.9786153672232878 1106 1 0.3370790536231354 1106 2 0.3786310485406581 1106 3 0.39268812376329976 1106 4 0.4183244011877747 1106 5 0.38568599312927176 1106 6 0.5151238706372325 1106 7 0.49878836726381337 1106 8 0.5136474568470106 1106 9 0.6474877626720357 1106 10 0.6456029099091509 1106 11 0.6050031436979211 1106 12 0.5775795723950949 1106 13 0.5303156107965421 1106 14 0.5659728688942636 1106 15 0.510719854601625 1106 16 0.6335682676454653 1106 17 0.6813778742854879 1106 18 0.6138178139624202 1106 19 0.6704669179734637 1106 20 0.7417769893925978 1106 21 0.6178364753877688 1106 22 0.6720576400262459 1106 23 0.6367975047058039 1106 24 0.6533771967224382 1106 25 0.7276701029515138 1106 26 0.7805427544044363 1106 27 0.8060455295604854 1106 28 0.722561472062047 1106 29 0.6939100138463417 1106 30 0.6350045182698949 1106 31 0.6717578513681772 1106 32 0.7667431707347916 1106 33 0.7101729146219569 1106 34 0.6930106942219467 1106 35 0.6719706568002195 1106 36 0.7380362054646454 1106 37 0.6847463929729827 1106 38 0.5614892852429776 1106 39 0.5982681035341813 1106 40 0.5867343875811457 1106 41 0.5559990027184796 1106 42 0.6660586893976159 1106 43 0.7137212996423727 1106 44 0.7637667207068759 1106 45 0.6801026289194851 1106 46 0.6961782492127497 1106 47 0.650556321754415 1106 48 0.696487147342502 1106 49 0.6222086850989754 1106 50 0.5911830404906055 1106 51 0.5767319428121809 1106 52 0.5390383955614553 1106 53 0.560768122762731 1106 54 0.6299752197669193 1106 55 0.5819904198546695 1106 56 0.5586085845385114 1106 57 0.5204667883807872 1106 58 0.5615730888536135 1106 59 0.6302798778169176 1106 60 0.5647586852189477 1106 61 0.6103347958988934 1106 62 0.5814156831645079 1106 63 0.6139981372244776 1106 64 0.617568726831216 1106 65 0.6029655391151252 1106 66 0.5958546156948401 1106 67 0.5641523713202048 1106 68 0.6880405604741497 1106 69 0.6010203704531989 1106 70 0.6241095323530956 1106 71 0.5908874521781229 1106 72 0.6313551924816849 1106 73 0.6048377917696826 1106 74 0.6445288852675901 1106 75 0.7286071125679072 1107 1 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-1.3152427114776373 2215 14 -1.5413385182713881 2215 15 -1.5608115367814506 2215 16 -1.5782495106822907 2215 17 -1.6705258687396665 2215 18 -1.5769889637125232 2215 19 -1.567941516617985 2215 20 -1.5450097896208064 2215 21 -1.5775092080050612 2215 22 -1.5339140843058772 2215 23 -1.507605445974356 2215 24 -1.5998168375194908 2215 25 -1.5839598801409167 2215 26 -1.6668677926861122 2215 27 -1.5716718981151487 2215 28 -1.5170665424792205 2215 29 -1.6549655823064597 2215 30 -1.503513171542501 2215 31 -1.5976027988219172 2215 32 -1.5669445607341217 2215 33 -1.5912368596954636 2215 34 -1.741179245080005 2215 35 -1.6528087808684262 2215 36 -1.6903011769305252 2215 37 -1.6569391931665933 2215 38 -1.7030721849452775 2215 39 -1.6666333805185332 2215 40 -1.7125493767331506 2215 41 -1.7049756068858741 2215 42 -1.7124584700802785 2215 43 -1.7944074340072262 2215 44 -1.7539281424983884 2215 45 -1.7272717354683067 2215 46 -1.6570294537696242 2215 47 -1.6869686955130234 2215 48 -1.7907333695316012 2215 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-1.4918742515868786 2216 47 -1.767911945500039 2216 48 -1.8567828806439408 2216 49 -1.8001462612518395 2216 50 -1.7107337928803474 2216 51 -1.747768101255808 2216 52 -1.7990686540878755 2216 53 -1.779117796062721 2216 54 -1.6973348452314028 2216 55 -1.6400355922903174 2216 56 -1.7638677318586815 2216 57 -1.6138918732808705 2216 58 -1.672463019961107 2216 59 -1.8374169772977247 2216 60 -1.7570031282063354 2216 61 -1.8421557255601222 2216 62 -1.7424671475016886 2216 63 -1.700650782115929 2216 64 -1.6815576350593098 2216 65 -1.601342885037063 2216 66 -1.6120273694413036 2216 67 -1.6278093329419931 2216 68 -1.7822061901980462 2216 69 -1.8738158587704454 2216 70 -1.623627471708744 2216 71 -1.6050053260850845 2216 72 -1.662781123282599 2216 73 -1.664073587631659 2216 74 -1.6821947289248662 2216 75 -1.6534200973895246 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75907.0 3 29171.0 4 17163.0 5 10979.0 6 8276.0 7 7583.0 8 7114.0 9 7105.0 10 8001.0 11 8505.0 12 9153.0 13 9326.0 14 10036.0 15 10637.0 16 11202.0 17 12159.0 18 12793.0 19 13742.0 20 15915.0 21 17901.0 22 22349.0 23 27151.0 24 28990.0 25 30594.0 26 32399.0 27 34200.0 28 39351.0 29 47130.0 30 54066.0 31 62275.0 32 75296.0 33 94539.0 34 122774.0 35 167023.0 36 234067.0 37 368740.0 38 634152.0 39 978358.0 40 249873.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.622806128976485 18.049347857519695 14.21511585148741 26.112730162016405 2 15.210016511312038 19.612459841565737 38.06222154669362 27.115302100428607 3 18.26749109512986 23.569979356009554 29.04194024668515 29.120589302175436 4 12.43214888409959 16.088215684003952 35.83019330573608 35.64944212616038 5 14.09482772979077 37.11057516675161 34.01552425018682 14.779072853270797 6 33.702769508625 36.043490917930995 16.47124578074323 13.782493792700764 7 28.998580282290256 31.50355817097751 20.612453681640368 18.885407865091867 8 26.87262123006313 34.88304100866919 18.94391976626232 19.300417995005365 9 26.216308300311912 15.618249244081808 18.607295990872935 39.55814646473335 10 14.385875268487904 29.174867285211757 33.23635451749017 23.202902928810172 11 35.66938074972153 22.778327571592772 22.53053204755544 19.021759631130266 12 23.626488939622924 24.503751567085704 29.85738270870139 22.012376784589982 13 28.40731734328712 21.54484463815889 25.624718446822914 24.423119571731075 14 22.190629897862273 20.77136137300094 26.862113654126752 30.175895075010033 15 23.823750412939347 28.734337775650015 23.526156708645253 23.91575510276538 16 23.663924776104828 27.966506991210636 25.043719339593856 23.32584889309068 17 22.537802194266416 27.710754479484436 26.397157006491156 23.354286319757993 18 22.480094439424022 27.066597434758076 27.583284932019037 22.870023193798865 19 23.434594285457536 26.727782503578556 27.67228317380404 22.165340037159865 20 23.37752567100454 26.139202396764745 27.925959228706372 22.55731270352434 21 23.587162997334822 25.8502597491939 27.849363220579328 22.713214032891948 22 23.196250297469966 25.76181434212957 27.827157329854113 23.214778030546352 23 23.117877703757962 25.75527161210498 27.754666424073726 23.37218426006333 24 22.88698591855579 25.924674661139157 27.766160431438564 23.422178988866484 25 23.234900430849656 25.7243094728679 27.85417788815438 23.186612208128064 26 22.991378375281883 26.107774331421147 27.84527624901762 23.05557104427935 27 23.123946575058227 26.140944512314878 27.584587456878236 23.150521455748663 28 23.074505238972552 26.06830291911572 27.626137464181372 23.231054377730352 29 23.198356550242693 26.14711583335322 27.580667268569414 23.073860347834678 30 23.21288081803403 26.203110349151395 27.651064986656458 22.932943846158114 31 23.21959989936878 25.794932546849132 27.65724806899285 23.328219484789237 32 22.94590103781411 26.163044343470737 27.618796419639786 23.27225819907537 33 23.211072814075827 25.956421998628187 27.577803892614583 23.2547012946814 34 23.314693065176776 25.986920933163514 27.57856426482531 23.119821736834396 35 23.249510787089218 26.095265617096047 27.565012069470452 23.09021152634428 36 23.269499899324224 26.050342325371904 27.4513719362531 23.228785839050776 37 23.140333132448422 25.829620937661986 27.654142190462743 23.37590373942685 38 23.249805735799576 25.96650946195611 27.598550603258982 23.18513419898533 39 23.37450442842345 26.1913616968759 27.31450473349724 23.119629141203404 40 23.336646690377876 25.869451143884746 27.69241692385861 23.101485241878763 41 23.15190313664343 26.0329977972994 27.495705260798974 23.319393805258194 42 23.420743982931473 25.98945392310677 27.323091959094782 23.266710134866976 43 23.156391802814472 25.952530786317535 27.41285102891935 23.47822638194864 44 23.296068638998772 26.086081083367286 27.182527851450793 23.43532242618315 45 23.256564040754544 26.195732298846686 27.101416558183494 23.446287102215273 46 23.394579118491098 25.90601313178262 27.032802833572017 23.666604916154267 47 23.171364492429873 26.177867595610095 26.971585080440853 23.67918283151918 48 23.138826134392676 26.35437391597116 27.03611608309145 23.470683866544714 49 23.364161230949104 26.188839337835727 26.912230482374465 23.534768948840703 50 23.510324222001973 26.218404619430025 26.84838488003004 23.422886278537963 51 23.27996019893875 26.443051924965904 26.6269709872671 23.650016888828247 52 23.42552259795739 26.39952699466621 26.568390120689017 23.60656028668738 53 23.353629181650987 26.40510572402823 26.69764658379719 23.543618510523594 54 23.268770424338157 26.360596473869048 26.842100592569256 23.52853250922354 55 23.229392073553395 26.181322742034823 26.827943257573043 23.761341926838732 56 23.15586898678253 26.477432008701822 26.758682546048153 23.608016458467496 57 23.294958825425017 26.304249797413437 26.62392335156104 23.77686802560051 58 23.194597199875098 26.2177126785436 26.919712854917186 23.667977266664114 59 23.348744927167253 26.384360213626824 26.766419088300363 23.500475770905563 60 23.23030243700604 26.409833105977054 26.923346776888472 23.436517680128432 61 23.37682434283724 26.182016641356686 26.963612423446097 23.477546592359975 62 23.165739142446697 26.424967622410033 26.783735451581737 23.625557783561533 63 23.054292035287784 26.258553549176895 27.012311453054316 23.674842962481005 64 23.328695114838204 26.146461386900366 26.915442327677518 23.60940117058391 65 23.240936155553662 26.168925491375155 26.862119283585102 23.728019069486077 66 23.237601314937667 26.340552435802206 26.710031947038587 23.711814302221537 67 23.238414284398797 26.29398000991849 26.663094235627344 23.804511470055374 68 23.221471483965452 26.299267198100594 26.87283307538263 23.606428242551328 69 23.238750118213996 26.306379062990796 26.64991379044964 23.804957028345566 70 23.04768291969232 26.04270069986279 26.882020157978776 24.027596222466112 71 23.209681378156994 26.14615530752132 26.712538671570034 23.931624642751657 72 23.060020957603843 26.395514029889828 26.620872064867612 23.92359294763872 73 23.119426198682756 26.23027155831809 26.734028379447256 23.9162738635519 74 23.167098486914057 26.32005660585125 26.530220529273585 23.982624377961105 75 23.23250615165703 26.313756278184407 26.574797854317556 23.878939715841003 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 836.0 1 598.0 2 468.5 3 615.5 4 654.0 5 516.0 6 475.0 7 946.5 8 1321.0 9 2210.5 10 3694.0 11 4142.5 12 3997.0 13 3815.0 14 3758.0 15 4270.5 16 4658.0 17 5053.5 18 6028.0 19 7438.0 20 8269.0 21 9356.0 22 12152.5 23 15732.5 24 17603.0 25 20148.5 26 25989.5 27 32443.0 28 35601.0 29 39621.0 30 48011.0 31 57240.0 32 62099.0 33 67343.5 34 78955.5 35 91147.0 36 96971.0 37 101756.0 38 110838.0 39 119939.0 40 124743.0 41 130637.5 42 140774.0 43 149387.0 44 153758.0 45 159051.0 46 167078.5 47 173628.0 48 177443.0 49 180852.0 50 185077.5 51 185816.0 52 185738.0 53 181509.5 54 174206.5 55 164783.5 56 158435.0 57 151314.5 58 134661.0 59 113204.0 60 101280.0 61 92612.5 62 73206.5 63 54408.5 64 46349.0 65 38439.5 66 25561.0 67 17878.0 68 15164.0 69 12743.5 70 8458.0 71 5306.5 72 4020.0 73 3569.0 74 2688.0 75 1766.5 76 1275.0 77 1010.5 78 580.5 79 317.5 80 220.0 81 169.0 82 87.0 83 42.0 84 28.0 85 22.0 86 13.5 87 7.0 88 3.0 89 1.5 90 1.5 91 1.5 92 0.0 93 0.0 94 1.0 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07537448055252434 2 0.016306179015777893 3 0.002856354487457692 4 0.13621760429506974 5 0.10069342858212504 6 0.0714365937834079 7 0.029201371604785916 8 0.025734921984084835 9 0.049057195032161716 10 0.03006105111071979 11 0.016722152970262024 12 0.10446492576944783 13 0.08907388945353502 14 0.04836390510802151 15 0.020632308142412844 16 0.03280647921031505 17 0.021658377230140365 18 0.019994481412203845 19 0.01364394570707946 20 0.012978387379904853 21 0.027149233429330878 22 0.0163339106127435 23 0.013976724870666766 24 0.02090962411206893 25 0.015557425897706458 26 0.033888011491973784 27 0.030144245901616615 28 0.03815867742467752 29 0.051830354728722584 30 0.03480315419183887 31 0.020854160918137712 32 0.012090976277005377 33 0.01530784152501598 34 0.014947330764463067 35 0.005629514184018558 36 0.010011106504584727 37 0.007820310344301643 38 0.0022462593542143016 39 0.008596795059338684 40 0.010732128025690551 41 0.011675002322521246 42 0.008153089507888947 43 0.011536344337693202 44 0.009650595744031813 45 0.005906830153674644 46 0.0024126489360079533 47 0.004936224259878341 48 0.006461462092986818 49 0.001719359011867737 50 2.4958437269047794E-4 51 0.0016916274149021283 52 0.0032168652480106044 53 8.319479089682598E-5 54 8.319479089682598E-5 55 4.437055514497386E-4 56 0.008679989850235511 57 0.003577376008563517 58 3.6051076055291256E-4 59 0.007432067986783122 60 0.0014975062361428676 61 0.0010815322816587378 62 0.024348342135804404 63 0.009095963804719642 64 0.012312829052730246 65 0.018136464415508063 66 0.018108732818542455 67 0.016833079358124458 68 0.018164196012473672 69 0.00682197285353973 70 0.01591793665825937 71 0.008596795059338684 72 0.017221321715642977 73 0.007958968329129686 74 0.019800360233444583 75 0.0015529694300740848 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 3605995.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.95079435205735 #Duplication Level Percentage of deduplicated Percentage of total 1 79.59809000692691 50.90361084849534 2 11.822534180613328 15.121209042091435 3 3.8814043857926985 7.4465668101900695 4 1.6734881966628885 4.280835980615347 5 0.8751208966463873 2.7982338247310574 6 0.5015853348022841 1.924606835756922 7 0.34779202435038054 1.5569103358561835 8 0.238910086879354 1.222279186772298 9 0.17521396991694047 1.008456530198924 >10 0.8193933622107102 9.161451365706236 >50 0.04501902988300101 1.9632886840995745 >100 0.020579209253350675 2.123772928246656 >500 6.954528570265187E-4 0.3114623176126145 >1k 1.7386320476721435E-4 0.17731530962751665 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1092638786243464E-4 2 0.0 0.0 0.0 0.0 1.1092638786243464E-4 3 0.0 0.0 0.0 0.0 1.1092638786243464E-4 4 0.0 0.0 0.0 0.0 1.1092638786243464E-4 5 0.0 0.0 0.0 0.0 1.1092638786243464E-4 6 0.0 0.0 0.0 0.0 1.1092638786243464E-4 7 0.0 0.0 0.0 0.0 1.1092638786243464E-4 8 0.0 0.0 0.0 0.0 1.1092638786243464E-4 9 0.0 0.0 0.0 0.0 1.1092638786243464E-4 10 0.0 0.0 0.0 0.0 1.1092638786243464E-4 11 0.0 0.0 0.0 0.0 1.1092638786243464E-4 12 0.0 0.0 0.0 0.0 1.386579848280433E-4 13 0.0 0.0 0.0 0.0 1.6638958179365196E-4 14 0.0 0.0 0.0 0.0 1.6638958179365196E-4 15 0.0 0.0 0.0 2.773159696560866E-5 2.2185277572486927E-4 16 0.0 0.0 0.0 2.773159696560866E-5 2.4958437269047794E-4 17 0.0 0.0 0.0 5.546319393121732E-5 2.4958437269047794E-4 18 0.0 0.0 0.0 5.546319393121732E-5 2.4958437269047794E-4 19 0.0 0.0 0.0 5.546319393121732E-5 2.4958437269047794E-4 20 0.0 0.0 0.0 5.546319393121732E-5 2.773159696560866E-4 21 0.0 0.0 0.0 1.9412117875926063E-4 2.773159696560866E-4 22 0.0 0.0 0.0 2.2185277572486927E-4 2.773159696560866E-4 23 0.0 0.0 0.0 2.4958437269047794E-4 3.050475666216953E-4 24 0.0 0.0 0.0 3.6051076055291256E-4 3.050475666216953E-4 25 0.0 0.0 0.0 3.8824235751852126E-4 3.050475666216953E-4 26 0.0 0.0 0.0 6.932899241402165E-4 3.050475666216953E-4 27 0.0 0.0 0.0 0.0010260690877275205 3.327791635873039E-4 28 0.0 0.0 0.0 0.002024406578489432 3.327791635873039E-4 29 0.0 0.0 0.0 0.0032445968449762133 3.327791635873039E-4 30 0.0 0.0 0.0 0.004991687453809559 3.327791635873039E-4 31 0.0 0.0 0.0 0.01014976448941277 3.327791635873039E-4 32 0.0 0.0 0.0 0.01575154707646572 3.327791635873039E-4 33 0.0 0.0 0.0 0.02323907825718006 3.327791635873039E-4 34 0.0 0.0 0.0 0.03197453130134679 3.8824235751852126E-4 35 0.0 0.0 0.0 0.042623464536140514 3.8824235751852126E-4 36 0.0 0.0 0.0 0.0563506050341168 4.159739544841299E-4 37 0.0 0.0 0.0 0.07501396979197142 4.159739544841299E-4 38 0.0 0.0 0.0 0.09547988835259062 4.4370555144973854E-4 39 0.0 0.0 0.0 0.11749877634328389 4.4370555144973854E-4 40 0.0 0.0 0.0 0.13901849558859622 4.4370555144973854E-4 41 0.0 0.0 0.0 0.15995585129763076 4.4370555144973854E-4 42 0.0 0.0 0.0 0.18089320700666528 4.7143714841534724E-4 43 0.0 0.0 0.0 0.20421548005474216 4.7143714841534724E-4 44 0.0 0.0 0.0 0.23225212458697253 4.991687453809559E-4 45 0.0 0.0 0.0 0.2622299809067955 4.991687453809559E-4 46 0.0 0.0 0.0 0.297837351410637 4.991687453809559E-4 47 0.0 0.0 0.0 0.33350018510840973 5.269003423465646E-4 48 0.0 0.0 0.0 0.3714370097573624 5.823635362777819E-4 49 0.0 0.0 0.0 0.4068779906794103 5.823635362777819E-4 50 0.0 0.0 0.0 0.4441215254042227 6.378267302089991E-4 51 0.0 0.0 0.0 0.4820306184562097 6.378267302089991E-4 52 0.0 0.0 0.0 0.522047312877583 6.655583271746078E-4 53 0.0 0.0 0.0 0.5637279031168928 6.932899241402165E-4 54 0.0 0.0 0.0 0.6065732204287583 7.210215211058251E-4 55 0.0 0.0 0.0 0.6556304154609199 7.210215211058251E-4 56 0.0 0.0 0.0 0.7066565538776399 7.210215211058251E-4 57 0.0 0.0 0.0 0.7648651759084525 7.210215211058251E-4 58 0.0 0.0 0.0 0.827899095811281 7.487531180714338E-4 59 0.0 0.0 0.0 0.8917372320261121 7.764847150370425E-4 60 0.0 0.0 0.0 0.9542165199896284 7.764847150370425E-4 61 0.0 0.0 0.0 1.0219648113766104 7.764847150370425E-4 62 0.0 0.0 0.0 1.0884374493031743 8.042163120026511E-4 63 0.0 0.0 0.0 1.1567403726294685 8.042163120026511E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3250 0.0 39.3678 1 GTATCAA 7045 0.0 34.898876 1 TATCAAC 9615 0.0 25.62876 2 ATCAACG 9965 0.0 24.52562 3 TCAACGC 9990 0.0 24.499529 4 CAACGCA 10035 0.0 24.328161 5 AACGCAG 10490 0.0 23.172897 6 GCACCGT 735 0.0 20.200598 6 ACGCAGA 12285 0.0 19.589195 7 CGAATCG 355 0.0 19.430794 37 CGCAGAG 12545 0.0 19.1009 8 GTACATG 9035 0.0 18.677317 1 CGTACAC 445 0.0 18.643572 3 GCGCGTT 150 1.700138E-5 18.39423 36 GAGTACT 8635 0.0 18.35386 12 AGAGTAC 12545 0.0 18.165627 11 TACATGG 9310 0.0 17.893064 2 AGTACTT 9235 0.0 17.48892 13 GTATACG 160 2.8951566E-5 17.289913 1 GTACTTT 9325 0.0 16.980213 14 >>END_MODULE