FastQCFastQC Report
Mon 6 Feb 2017
SRR2077492_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077492_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3376240
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34150.039.735851
GTATCAA68750.035.0058561
ATCAACG90000.026.6037523
TCAACGC90550.026.4445154
TATCAAC92150.026.0571522
CAACGCA92550.025.6524125
AACGCAG95200.024.8773526
ACGCAGA112150.020.812247
GTGGTAT15700.020.4533271
CGCAGAG114500.020.2062238
GAGTACT65450.019.75704212
AGAGTAC113100.019.6349611
GTACATG108600.019.2038271
TACATGG110000.018.4729232
CAGAGTA121500.018.41911510
AGTACTT71650.018.2410213
GTACTTT71900.017.93778414
GCAGAGT127450.017.7751379
TGGTATC16500.017.7724782
ACATGGG113450.017.5163613
Produced by FastQC (version 0.11.5)