FastQCFastQC Report
Mon 6 Feb 2017
SRR2077492_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077492_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3376240
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38700.039.870121
GTATCAA80900.035.7504431
ATCAACG113550.025.4624883
TATCAAC113900.025.2890192
TCAACGC114800.025.2161164
CAACGCA117250.024.7365445
AACGCAG121550.023.8586226
GTGGTAT19000.021.3037431
ACGCAGA144400.019.9621897
CGCAGAG146600.019.6161048
GTACATG101600.019.5452771
GAGTACT100550.019.33097612
TGGTATC20000.019.1912692
AGAGTAC144050.018.79015211
TACGCTA4450.018.60037431
TACATGG103350.018.5022852
AGTACTT105700.018.41440613
GGACCGT3400.018.2774096
CAGAGTA152200.018.05618110
GCAGAGT155100.018.0074149
Produced by FastQC (version 0.11.5)