##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077492_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3376240 Sequences flagged as poor quality 0 Sequence length 75 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.961552496268038 33.0 31.0 34.0 28.0 34.0 2 31.051171125275456 33.0 31.0 34.0 27.0 34.0 3 31.130671101580457 33.0 31.0 34.0 27.0 34.0 4 34.471239900007106 37.0 35.0 37.0 31.0 37.0 5 34.19819947634054 37.0 35.0 37.0 30.0 37.0 6 34.174793261142575 37.0 35.0 37.0 30.0 37.0 7 34.0143422860934 37.0 35.0 37.0 30.0 37.0 8 33.96723485297254 37.0 35.0 37.0 30.0 37.0 9 35.447857379807125 39.0 37.0 39.0 29.0 39.0 10 35.34168216714452 39.0 35.0 39.0 29.0 39.0 11 35.33421587327915 39.0 37.0 39.0 29.0 39.0 12 35.231362106959224 39.0 35.0 39.0 28.0 39.0 13 35.16035204843258 39.0 35.0 39.0 27.0 39.0 14 36.420836492666396 40.0 37.0 41.0 27.0 41.0 15 36.47553609932943 40.0 37.0 41.0 28.0 41.0 16 36.45859002914485 40.0 37.0 41.0 28.0 41.0 17 36.46374754164396 40.0 37.0 41.0 28.0 41.0 18 36.47021449896927 40.0 37.0 41.0 28.0 41.0 19 36.510878077387865 40.0 37.0 41.0 28.0 41.0 20 36.48576019477289 40.0 37.0 41.0 28.0 41.0 21 36.46436864677867 40.0 37.0 41.0 28.0 41.0 22 36.44684738051797 40.0 37.0 41.0 28.0 41.0 23 36.41995859299102 40.0 37.0 41.0 28.0 41.0 24 36.40551116034405 40.0 37.0 41.0 28.0 41.0 25 36.375401926403335 40.0 37.0 41.0 28.0 41.0 26 36.307105833708505 40.0 37.0 41.0 28.0 41.0 27 36.261883041489945 40.0 36.0 41.0 28.0 41.0 28 36.243621010354715 40.0 36.0 41.0 28.0 41.0 29 36.1869517569841 40.0 36.0 41.0 28.0 41.0 30 36.11568371916688 40.0 36.0 41.0 27.0 41.0 31 36.02221376442433 40.0 36.0 41.0 27.0 41.0 32 35.95052691751771 40.0 36.0 41.0 27.0 41.0 33 35.885934649195555 40.0 36.0 41.0 27.0 41.0 34 35.827219628936334 40.0 35.0 41.0 27.0 41.0 35 35.743826268274766 40.0 35.0 41.0 26.0 41.0 36 35.684201360092885 40.0 35.0 41.0 26.0 41.0 37 35.62278155581357 40.0 35.0 41.0 26.0 41.0 38 35.56426527734995 40.0 35.0 41.0 25.0 41.0 39 35.49043225600076 40.0 35.0 41.0 25.0 41.0 40 35.43045044191171 40.0 35.0 41.0 25.0 41.0 41 35.376102409781296 40.0 35.0 41.0 25.0 41.0 42 35.31438108665261 40.0 35.0 41.0 25.0 41.0 43 35.22337600407554 40.0 35.0 41.0 24.0 41.0 44 35.09391364357984 40.0 35.0 41.0 24.0 41.0 45 35.005176764684975 40.0 35.0 41.0 23.0 41.0 46 34.87018102978461 40.0 35.0 41.0 23.0 41.0 47 34.7253811932801 39.0 35.0 41.0 22.0 41.0 48 34.62460903253323 39.0 35.0 41.0 21.0 41.0 49 34.53246481292799 39.0 34.0 41.0 20.0 41.0 50 34.53770259223278 39.0 35.0 41.0 20.0 41.0 51 34.46676776532474 39.0 34.0 41.0 19.0 41.0 52 34.40257949671824 39.0 34.0 41.0 19.0 41.0 53 34.3100999928915 39.0 34.0 41.0 18.0 41.0 54 34.27607427197119 39.0 34.0 41.0 18.0 41.0 55 34.200894782361445 39.0 34.0 41.0 17.0 41.0 56 34.081537746131794 39.0 34.0 41.0 15.0 41.0 57 34.013855946259746 39.0 34.0 41.0 15.0 41.0 58 33.920367924081226 39.0 34.0 41.0 14.0 41.0 59 33.83517552069758 38.0 34.0 41.0 13.0 41.0 60 33.80862438689193 38.0 34.0 41.0 13.0 41.0 61 33.69710299030874 38.0 34.0 41.0 11.0 41.0 62 33.55835781816459 38.0 34.0 41.0 8.0 41.0 63 33.4277258133308 38.0 34.0 40.0 8.0 41.0 64 33.28291561026467 37.0 34.0 40.0 7.0 41.0 65 33.1609011800109 37.0 34.0 40.0 2.0 41.0 66 32.96781212236097 37.0 33.0 40.0 2.0 41.0 67 32.78255189204559 37.0 33.0 40.0 2.0 41.0 68 32.57510840461578 36.0 33.0 39.0 2.0 41.0 69 32.41514317702533 36.0 33.0 39.0 2.0 41.0 70 32.22776787195223 36.0 33.0 39.0 2.0 41.0 71 32.0540459209061 36.0 33.0 39.0 2.0 41.0 72 31.8577399710921 35.0 33.0 39.0 2.0 41.0 73 31.66138307703244 35.0 33.0 39.0 2.0 41.0 74 31.397682036822026 35.0 32.0 38.0 2.0 40.0 75 31.181036893111862 35.0 32.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4941303122596139 1101 2 0.5067066340778545 1101 3 0.5589801131756644 1101 4 0.5747206281487465 1101 5 0.5056396003558987 1101 6 0.4946669865710547 1101 7 0.6101340913254845 1101 8 0.6431301042803312 1101 9 0.7013900425475299 1101 10 0.7734214315596688 1101 11 0.7422915009157123 1101 12 0.8276674564987943 1101 13 0.7518515051120787 1101 14 0.7917806851184395 1101 15 0.7983134486495018 1101 16 0.8167974945276271 1101 17 0.8256332900962775 1101 18 0.833877168073542 1101 19 0.8556224793620402 1101 20 0.8686986599391702 1101 21 0.7679678667960061 1101 22 0.8469222158785712 1101 23 0.8997842656379476 1101 24 0.8436355051460751 1101 25 0.8798921876292027 1101 26 0.8734415875579984 1101 27 0.8048672463644522 1101 28 0.8016688045547866 1101 29 0.8477173851063569 1101 30 0.8144553581924185 1101 31 0.8761016048881913 1101 32 0.8554739675456133 1101 33 0.8836581786035751 1101 34 0.7809242296269048 1101 35 0.9525041545409536 1101 36 0.9290367032524998 1101 37 0.984776425782627 1101 38 0.9784966097501098 1101 39 0.9553796803457857 1101 40 0.8252396524224608 1101 41 0.9030642429291618 1101 42 0.9251347054776531 1101 43 1.0589451153180107 1101 44 1.0293217958321748 1101 45 1.0344594637131692 1101 46 1.05754758247447 1101 47 0.9615466311103944 1101 48 1.0748145822257698 1101 49 1.133924703683718 1101 50 1.0444536185653206 1101 51 1.0804446573494673 1101 52 1.1216484739915913 1101 53 1.0557857728085196 1101 54 0.9970953148894637 1101 55 1.1609510371586467 1101 56 1.1981322805026977 1101 57 1.0904756466982661 1101 58 1.0599905883245881 1101 59 1.1644677936313244 1101 60 0.9957429713488537 1101 61 1.0892193217339141 1101 62 1.151970410621395 1101 63 1.0551408386254195 1101 64 1.0922537306426747 1101 65 0.9631036963789796 1101 66 1.1210208491172935 1101 67 1.163005020380659 1101 68 1.071310189748381 1101 69 1.0426768506835629 1101 70 1.0220471130214364 1101 71 0.9666478788774597 1101 72 0.8840213042250262 1101 73 0.8106765440675545 1101 74 0.9433377562603162 1101 75 0.9906107492092424 1102 1 0.6621212326856245 1102 2 0.6903919457339711 1102 3 0.6880070740858351 1102 4 0.6635434566414347 1102 5 0.769850621452477 1102 6 0.8052757893865632 1102 7 0.8140045493989447 1102 8 0.8442248670672399 1102 9 0.9416701716312659 1102 10 1.019244788026434 1102 11 1.0490602864568572 1102 12 1.076625161957729 1102 13 1.1051350429859852 1102 14 1.2505439091571873 1102 15 1.2083631782767483 1102 16 1.1510422085328926 1102 17 1.1459237943312672 1102 18 1.1686028783040001 1102 19 1.1655684838096576 1102 20 1.2214763433574944 1102 21 1.1825224660294609 1102 22 1.2258636081238024 1102 23 1.28388152873098 1102 24 1.2544828574417082 1102 25 1.2808455955635552 1102 26 1.2770202774186572 1102 27 1.289037003851874 1102 28 1.273542954496783 1102 29 1.284174665947745 1102 30 1.3994273466006888 1102 31 1.2734033809086895 1102 32 1.4005775149783588 1102 33 1.374234302325938 1102 34 1.3721324212564738 1102 35 1.3547734160487437 1102 36 1.3237619131583998 1102 37 1.2870015270654278 1102 38 1.3703107734115392 1102 39 1.3971420340831386 1102 40 1.318029001302719 1102 41 1.281885504616021 1102 42 1.256928039837966 1102 43 1.2666573286353326 1102 44 1.399912244636461 1102 45 1.3523716356878879 1102 46 1.334765145972895 1102 47 1.3992818461907035 1102 48 1.4755938245500673 1102 49 1.4552401359312128 1102 50 1.5063109549690736 1102 51 1.3762347948992186 1102 52 1.3522544328570092 1102 53 1.3742061165314894 1102 54 1.5111069039860752 1102 55 1.395409600410865 1102 56 1.4582599159293608 1102 57 1.4802396910059485 1102 58 1.4370330645624634 1102 59 1.5253808596070186 1102 60 1.4510585905597395 1102 61 1.5072181025219749 1102 62 1.517622919419864 1102 63 1.4943053492882612 1102 64 1.5642126197641701 1102 65 1.5070205793043527 1102 66 1.4654383199240186 1102 67 1.5290722192346493 1102 68 1.4746874331444246 1102 69 1.4899836957817598 1102 70 1.547894801061176 1102 71 1.548345164090108 1102 72 1.4352679418465364 1102 73 1.5303214189666718 1102 74 1.568001176083964 1102 75 1.6037521685773335 1103 1 0.44443099610370496 1103 2 0.45973604884131447 1103 3 0.4681959865152123 1103 4 0.4271463285811734 1103 5 0.5436209251247988 1103 6 0.5419431916935338 1103 7 0.6002942739752157 1103 8 0.5942414925380533 1103 9 0.7661896513864477 1103 10 0.8018382143446985 1103 11 0.7274021532176889 1103 12 0.7021381975038707 1103 13 0.6694714954341947 1103 14 0.7476841810498414 1103 15 0.8056757371279701 1103 16 0.8002808208210297 1103 17 0.676767426628814 1103 18 0.7297900546222635 1103 19 0.8347681071618283 1103 20 0.8056611067755739 1103 21 0.806292034061471 1103 22 0.8195016436699447 1103 23 0.9067527328270302 1103 24 0.8303779845603501 1103 25 0.9098536581812624 1103 26 0.9278131505354139 1103 27 0.922652381676734 1103 28 0.9314182781785192 1103 29 0.8841571577085716 1103 30 0.9076528350658535 1103 31 0.8980720485169726 1103 32 0.958486530985887 1103 33 0.9699153581168929 1103 34 0.960659522245173 1103 35 1.0360693200035485 1103 36 1.0779485892412666 1103 37 1.0956295579034716 1103 38 1.0750151931384693 1103 39 1.088906540909882 1103 40 1.0583804902386902 1103 41 1.0011011649267516 1103 42 0.9822268569283636 1103 43 1.0103584821193294 1103 44 1.0763708722259153 1103 45 1.03957767382348 1103 46 1.0150695539295853 1103 47 1.0649069933187647 1103 48 1.0738737503975244 1103 49 1.1021632468069171 1103 50 1.1774375145265097 1103 51 1.144676267356843 1103 52 1.0531591232995723 1103 53 1.0478537154015442 1103 54 1.0112010659446469 1103 55 1.0537881020586966 1103 56 1.0289918780291742 1103 57 0.9778013376632018 1103 58 0.9756416744965506 1103 59 0.9800801298518351 1103 60 0.9798096204561588 1103 61 1.0454640417875112 1103 62 1.0670967894763592 1103 63 1.0082497054558672 1103 64 0.9562549043686914 1103 65 0.9447365632968214 1103 66 0.9985602302347516 1103 67 0.9993354313344653 1103 68 1.0072187714212646 1103 69 0.9779475905557717 1103 70 1.0448087899971483 1103 71 0.9715393142784308 1103 72 1.0010589022985137 1103 73 0.9853417226389851 1103 74 1.0538804133720951 1103 75 1.0624047698014358 1104 1 0.3444299240908286 1104 2 0.3545922012056799 1104 3 0.3422410704290826 1104 4 0.392229494790449 1104 5 0.31774698724216677 1104 6 0.380664489235933 1104 7 0.3904197159743745 1104 8 0.4337000050899462 1104 9 0.58231607853795 1104 10 0.5690076000758211 1104 11 0.6060467221255763 1104 12 0.5857177364613619 1104 13 0.5792923978161539 1104 14 0.6529343228853932 1104 15 0.6053921210031419 1104 16 0.62905768711299 1104 17 0.7520010154860373 1104 18 0.6351146876090326 1104 19 0.6600706896683377 1104 20 0.7985623199643044 1104 21 0.7419079027107571 1104 22 0.7511436430454239 1104 23 0.7080374165667394 1104 24 0.7135005436694968 1104 25 0.7891073503035599 1104 26 0.7139772300776741 1104 27 0.8135086890706518 1104 28 0.8017873199859196 1104 29 0.7281950893724272 1104 30 0.7547152174193101 1104 31 0.7142240390524748 1104 32 0.7311763707227144 1104 33 0.6707747001318864 1104 34 0.7591347076209374 1104 35 0.7466753206368608 1104 36 0.7199017857087853 1104 37 0.737069974877997 1104 38 0.7580359659992837 1104 39 0.6582870768943323 1104 40 0.7098766628778392 1104 41 0.7077625704840713 1104 42 0.7032276716581478 1104 43 0.6981657924775035 1104 44 0.7719178406834502 1104 45 0.7899908110939009 1104 46 0.7904926141910025 1104 47 0.7941636815796187 1104 48 0.8022821741097914 1104 49 0.7881867552246931 1104 50 0.7349412473303261 1104 51 0.744153702310399 1104 52 0.6854734121867025 1104 53 0.6841708728851827 1104 54 0.7224986523213914 1104 55 0.6950218129604622 1104 56 0.7948083285340672 1104 57 0.7566111339917541 1104 58 0.7156990299342354 1104 59 0.7054991494493308 1104 60 0.7155734268825213 1104 61 0.7061497012238647 1104 62 0.6710556505698833 1104 63 0.6487767124838726 1104 64 0.6345871327041337 1104 65 0.5420033152847168 1104 66 0.5393457847787673 1104 67 0.5579491097789742 1104 68 0.6001907374598972 1104 69 0.5687079115659088 1104 70 0.5760861400692718 1104 71 0.597238871399675 1104 72 0.6699157737361325 1104 73 0.6251462204751661 1104 74 0.7043151558251246 1104 75 0.7059057879387964 1105 1 0.3995355851254665 1105 2 0.4334652946327324 1105 3 0.43893319396748254 1105 4 0.36769548287301745 1105 5 0.38124757819056043 1105 6 0.4935202246293926 1105 7 0.5453301046567987 1105 8 0.6106468247622132 1105 9 0.7264744612874452 1105 10 0.7983567698118605 1105 11 0.740866815818336 1105 12 0.8029337309472595 1105 13 0.7161026165535205 1105 14 0.8168909393729109 1105 15 0.8622842399736115 1105 16 0.8922870021749603 1105 17 0.8711043220016563 1105 18 0.8923093991984388 1105 19 0.869414768810131 1105 20 0.8867248161011716 1105 21 0.8942166969615144 1105 22 0.9313222489671631 1105 23 0.9251025914717275 1105 24 0.889734914177815 1105 25 0.9505493536521854 1105 26 0.8975543038891658 1105 27 0.9408528121581554 1105 28 0.913063696997952 1105 29 0.9503957576851931 1105 30 0.9231624002503906 1105 31 0.9514960474106502 1105 32 0.9191745385248424 1105 33 0.9073433171687668 1105 34 0.9311765971527919 1105 35 0.8786035788826254 1105 36 0.9629079718686029 1105 37 0.8482460655365145 1105 38 0.8940079225717383 1105 39 0.9458273815854241 1105 40 0.8811046467403187 1105 41 0.9016559203243446 1105 42 0.8912631976438234 1105 43 0.9054068052448585 1105 44 0.9926845961646436 1105 45 0.9518713613790766 1105 46 0.9587276961516906 1105 47 0.9942063097807079 1105 48 0.8564738164773829 1105 49 0.9066736517117988 1105 50 0.8608277945140799 1105 51 0.9180264895476995 1105 52 0.8868445528685669 1105 53 0.8194216258237716 1105 54 0.8894658530063921 1105 55 0.8637579366380663 1105 56 0.9691278104324041 1105 57 0.9658995225699059 1105 58 0.9582668858523036 1105 59 0.9497081651058323 1105 60 0.9457755073519607 1105 61 0.9993083820153927 1105 62 0.9807363997548819 1105 63 0.9369630710521406 1105 64 0.9034734822188923 1105 65 0.8883920769376417 1105 66 0.9312554064049436 1105 67 1.0520827264797674 1105 68 0.9740511990969623 1105 69 0.9513489765220342 1105 70 0.9833197245388803 1105 71 0.8802426271106114 1105 72 0.8827994757604962 1105 73 0.9253493891858966 1105 74 0.9092350205711703 1105 75 0.9504759471492328 1106 1 0.20286312442095422 1106 2 0.2859715358738839 1106 3 0.2775389448988079 1106 4 0.26930262465408816 1106 5 0.2900759286430912 1106 6 0.33054522434666467 1106 7 0.4454217493471475 1106 8 0.4047857497589362 1106 9 0.4193857502672884 1106 10 0.43673001629819197 1106 11 0.4126304444990154 1106 12 0.398919533367021 1106 13 0.3746137560225904 1106 14 0.4370069406497663 1106 15 0.44182695261517324 1106 16 0.4073901329943297 1106 17 0.4827517744039156 1106 18 0.4801934543843629 1106 19 0.4918683618193782 1106 20 0.4874132491660035 1106 21 0.4676029864304567 1106 22 0.57135058106104 1106 23 0.4872533673766881 1106 24 0.5309553619226364 1106 25 0.510722446714972 1106 26 0.5632206549751686 1106 27 0.6026877845215282 1106 28 0.6302917431128421 1106 29 0.5383278557550852 1106 30 0.5258264524596328 1106 31 0.6196879930954466 1106 32 0.6620439880961939 1106 33 0.5844664927670777 1106 34 0.5733384212701012 1106 35 0.6419134595290785 1106 36 0.6220885336186939 1106 37 0.6536026324745734 1106 38 0.5777562696530083 1106 39 0.5839037032373184 1106 40 0.550159878474183 1106 41 0.49648889989860834 1106 42 0.48863910687810375 1106 43 0.5631842176105479 1106 44 0.5843441038637636 1106 45 0.5984384372935239 1106 46 0.5119923545762788 1106 47 0.7172557170990927 1106 48 0.557142215164312 1106 49 0.5529613029488587 1106 50 0.5440378466703208 1106 51 0.5652044200005903 1106 52 0.5290349798369007 1106 53 0.5574599176532544 1106 54 0.6191289576481722 1106 55 0.6663031281108331 1106 56 0.6094056023121155 1106 57 0.5778054737542107 1106 58 0.6418431618139309 1106 59 0.6513886424506836 1106 60 0.665464347955826 1106 61 0.6895083848715515 1106 62 0.6324051429142372 1106 63 0.7570248466065195 1106 64 0.6342955595255049 1106 65 0.595678754357003 1106 66 0.625646861969777 1106 67 0.5974113113270931 1106 68 0.7090487560915477 1106 69 0.6278230366253368 1106 70 0.6838696669740898 1106 71 0.6891244025708829 1106 72 0.7013504181811037 1106 73 0.6387252545046849 1106 74 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-1.7813879069910676 2216 75 -1.7449281717247196 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 73497.0 3 29198.0 4 16612.0 5 10345.0 6 7572.0 7 6942.0 8 6545.0 9 6769.0 10 7320.0 11 7863.0 12 8267.0 13 8751.0 14 9362.0 15 10000.0 16 10754.0 17 11639.0 18 12400.0 19 13218.0 20 15362.0 21 17534.0 22 21900.0 23 26922.0 24 28462.0 25 29790.0 26 30772.0 27 32052.0 28 36318.0 29 43897.0 30 50118.0 31 56663.0 32 68612.0 33 85481.0 34 111202.0 35 150402.0 36 212241.0 37 328546.0 38 558956.0 39 937572.0 40 276384.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.54239193196329 18.559401109995292 14.41660036968226 25.481606588359156 2 14.863145097360778 19.917486547943355 38.78378122174653 26.435587132949333 3 18.018227755118964 24.18879973461052 29.721163330045584 28.07180918022493 4 12.101673167712143 16.414282150811403 36.800050066602225 34.68399461487423 5 13.448088473384667 37.82155369182135 34.34551711113753 14.384840723656456 6 32.3692092835223 37.04117217124712 16.83926214100981 13.750356404220772 7 28.0743632801899 32.447467875143545 20.902310572079017 18.57585827258754 8 26.39370479043619 35.260526777334306 19.361840047022685 18.983928385206813 9 25.982574094767696 15.862030544969214 19.228383784775282 38.92701157548781 10 14.222595525691279 29.705001377667568 33.48543968334385 22.586963413297305 11 34.84778555427648 23.39839049583227 22.900449033059235 18.853374916832017 12 23.096861190769296 25.33423823450579 30.134928710865694 21.43397186385922 13 27.76072723930982 22.20492958685918 26.12224509790843 23.912098075922575 14 21.527690731687947 21.43325128035538 27.695058063816415 29.343999924140256 15 23.25205096932426 29.454890820858758 24.215672536933443 23.077385672883537 16 22.7211397101305 28.73727398942988 25.79268505860735 22.74890124183227 17 21.670078537211115 28.29046029145571 27.23315567906099 22.806305492272188 18 21.606503606465687 27.587566551052788 28.639111696751158 22.166818145730364 19 22.47876494540827 27.114426092175275 28.969786591922592 21.437022370493864 20 22.70860653880231 26.458987557678633 28.91069036028908 21.92171554322998 21 22.94956125407445 26.267545837379885 28.85036657460577 21.932526333939897 22 22.46268986112769 26.25308418335683 28.81860336150436 22.465622594011123 23 22.341602570084117 26.252627182283128 28.70096111548049 22.704809132152267 24 22.088217065838105 26.448855455738006 28.88541877747007 22.577508700953818 25 22.35810856246873 26.375264130637316 28.844887441023292 22.421739865870663 26 22.091750947745485 26.570116870091837 28.93089568799878 22.4072364941639 27 22.186794309859856 26.78578306385142 28.576113949755307 22.451308676533415 28 22.17518685914853 26.607717565796996 28.789316272997763 22.42777930205671 29 22.244190681086433 26.609947740948016 28.824889947800216 22.32097163016534 30 22.372674355557162 26.633099126955578 28.668128752867332 22.326097764619924 31 22.359265500169453 26.340334212590683 28.783561679547432 22.516838607692435 32 22.037642264886916 26.727477336704748 28.690311398697744 22.54456899971059 33 22.171741423637513 26.455842104935744 28.68665761586795 22.68575885555879 34 22.36340428279898 26.6072197176181 28.708450750536983 22.320925249045924 35 22.520415196625397 26.600818296234134 28.443658381908506 22.435108125231963 36 22.39801653773866 26.701146207768854 28.537566852245984 22.363270402246503 37 22.503191675281474 26.29759743125679 28.61121396220938 22.587996931252352 38 22.270557915405 26.511711392568532 28.68160455808187 22.5361261339446 39 22.465963533690488 26.79359040361523 28.386925352302082 22.353520710392193 40 22.592076401159407 26.564332400732848 28.59193125317511 22.25165994493263 41 22.36863464393581 26.603772914215433 28.37923581957977 22.648356622268988 42 22.424043514488687 26.605349144648116 28.250975494812025 22.719631846051175 43 22.321739339077453 26.44676596194685 28.3829557946323 22.848538904343403 44 22.33649970259747 26.740308350731183 28.283039524914273 22.64015242175707 45 22.614758303485846 26.65930501070481 28.041511208950574 22.68442547685877 46 22.622459137832934 26.576736281114055 27.93626618588476 22.86453839516825 47 22.56815400879189 26.790582058946104 27.816302223859896 22.824961708402114 48 22.44608523105441 26.956481042931284 27.7628144121056 22.834619313908703 49 22.557610040427214 26.792056492064575 27.743898375417743 22.906435092090465 50 22.70964279399129 26.96711214368922 27.641858628749038 22.681386433570452 51 22.53883692991581 27.061153354927836 27.426567635259858 22.973442079896497 52 22.665115778978954 27.094920191189264 27.359661103197713 22.880302926634066 53 22.637430988318368 26.96230125820439 27.593802573276783 22.806465180200462 54 22.554755486655704 27.059237843788807 27.664645580271763 22.72136108928372 55 22.466222620300076 26.96610177523766 27.609483257035077 22.958192347427183 56 22.51397826274987 27.23012385232509 27.4098655314228 22.84603235350224 57 22.556728598725343 27.02312922026741 27.306176115237648 23.1139660657696 58 22.45607965817454 26.869054382023005 27.66660170261114 23.008264257191314 59 22.507702182855347 27.040089858059456 27.50688612617747 22.94532183290773 60 22.39265820700212 27.232331944583493 27.617945499319053 22.757064349095337 61 22.645409651019207 26.969586840601707 27.620762140191214 22.764241368187875 62 22.445885233590037 27.22291595380192 27.537272642435934 22.79392617017211 63 22.317165213640987 26.901127506854376 27.703925183593093 23.077782095911545 64 22.354444928270343 26.894489420504936 27.719224970155842 23.03184068106888 65 22.359948524950703 26.900719750113755 27.619670009858943 23.119661715076596 66 22.43152617597944 27.16817853125621 27.450021966250475 22.95027332651387 67 22.40821311779903 27.09785574609863 27.413170647749354 23.08076048835299 68 22.488583697089897 26.958975369224643 27.580158517384035 22.97228241630143 69 22.431594088193684 27.110855846996344 27.340060378717684 23.11748968609228 70 22.367582641708637 26.748894392855867 27.56462495560242 23.318898009833084 71 22.455207860940057 26.73553154740412 27.491311419518404 23.31794917213742 72 22.313996459967267 27.048486598976503 27.354854954934936 23.282661986121294 73 22.465900393128894 26.74958945400345 27.45243472140929 23.332075431458367 74 22.374577075061346 27.051347035000305 27.208208083957842 23.365867805980507 75 22.355084214875664 26.975802570060587 27.355609956207967 23.313503258855782 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 947.0 1 666.0 2 489.5 3 620.5 4 647.0 5 528.0 6 513.0 7 1048.5 8 1480.0 9 2375.5 10 3970.0 11 4640.0 12 4611.0 13 4319.5 14 4173.5 15 4696.0 16 5073.0 17 5650.0 18 6789.0 19 8353.0 20 9355.0 21 10747.5 22 14373.0 23 18867.0 24 21128.0 25 24133.5 26 31523.0 27 39103.0 28 42299.0 29 47570.0 30 57482.5 31 67369.0 32 72614.0 33 77504.5 34 88568.0 35 100449.0 36 106157.0 37 110806.5 38 119382.5 39 126047.0 40 128785.0 41 134205.5 42 142294.5 43 147735.0 44 150507.0 45 154637.0 46 160250.0 47 162441.0 48 163149.0 49 164087.5 50 162573.5 51 158066.0 52 156011.0 53 152834.0 54 145458.5 55 134398.5 56 127537.0 57 120936.5 58 105743.5 59 88926.5 60 80702.0 61 72385.5 62 55758.0 63 40799.0 64 34151.0 65 28857.0 66 19691.5 67 13455.5 68 11091.0 69 9329.5 70 6155.0 71 3887.5 72 3033.0 73 2707.5 74 2168.0 75 1459.5 76 965.0 77 777.5 78 447.0 79 251.5 80 199.0 81 156.0 82 72.0 83 25.0 84 19.0 85 12.5 86 5.5 87 6.0 88 7.0 89 5.0 90 2.5 91 2.5 92 3.0 93 2.0 94 0.5 95 0.0 96 0.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07499466862545316 2 0.016231073620358742 3 0.0020733123237684525 4 0.14012629433926496 5 0.09486884818614791 6 0.06699760680520342 7 0.028789422552899086 8 0.02511669786508068 9 0.047656564699192 10 0.02873018505793427 11 0.016053361135464305 12 0.103547141198493 13 0.08693102386086297 14 0.04712342724450869 15 0.018985617136222543 16 0.03157358481624529 17 0.022806435561452978 18 0.01883752339881051 19 0.013446911357012535 20 0.012528730185057933 21 0.027782385138497262 22 0.01626069236784115 23 0.014068905054143072 24 0.022154823116840035 25 0.01608297988294671 26 0.033321090917707276 27 0.029559509987441654 28 0.03666800938321921 29 0.050499964457503016 30 0.03418003459469706 31 0.019666848328317894 32 0.01205483022533943 33 0.014187380044072695 34 0.013535767599459755 35 0.0059533682439637 36 0.0096260929317821 37 0.007997061820249745 38 0.002458356041039737 39 0.008944861739686751 40 0.011107030305902424 41 0.011492074023173707 42 0.007730493092908087 43 0.011343980285761675 44 0.010188849133947825 45 0.005568324526692415 46 0.002162168566215672 47 0.005124043314456319 48 0.006427268203682202 49 0.0014513186266379167 50 2.6656872734165816E-4 51 0.0021325498187332653 52 0.003228443475582305 53 0.0 54 8.885624244721938E-5 55 4.738999597185034E-4 56 0.008411724285003436 57 0.003583868445371182 58 4.146624647536905E-4 59 0.0073750681231192095 60 0.0018363623439092008 61 0.0010958936568490393 62 0.02295452929886501 63 0.008767149254792314 64 0.010958936568490393 65 0.018482098429021633 66 0.017978579721820723 67 0.016793829822524465 68 0.01732696727720778 69 0.006397649456199796 70 0.014602042508826386 71 0.007612018102978461 72 0.016734592327559653 73 0.007345449375636803 74 0.018985617136222543 75 0.0016290311115323556 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 3376240.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.41508707400811 #Duplication Level Percentage of deduplicated Percentage of total 1 75.48751779129647 44.850974425706156 2 13.126684455716346 15.598461998588306 3 4.846405776986459 8.638488637068791 4 2.3080452391726456 5.485308354247705 5 1.278230701389168 3.7973094211853935 6 0.7670788807514135 2.734563509548675 7 0.4804213953775377 1.9980995327001014 8 0.3498043825397496 1.6626926277975094 9 0.23861635084496793 1.2759670136462253 >10 1.0612094015978668 10.71476381473284 >50 0.0428466551461483 1.6878509060272642 >100 0.012738608242441121 1.2966822428473157 >500 2.5022558830768433E-4 0.08611067819017242 >1k 1.5013535055011346E-4 0.17272683771366132 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.9618747482406463E-5 0.0 4 0.0 0.0 0.0 2.9618747482406463E-5 0.0 5 0.0 0.0 0.0 2.9618747482406463E-5 0.0 6 0.0 0.0 0.0 5.9237494964812926E-5 0.0 7 0.0 0.0 0.0 5.9237494964812926E-5 0.0 8 0.0 0.0 0.0 5.9237494964812926E-5 0.0 9 0.0 0.0 0.0 5.9237494964812926E-5 0.0 10 0.0 0.0 0.0 8.88562424472194E-5 0.0 11 0.0 0.0 0.0 1.1847498992962585E-4 0.0 12 2.9618747482406463E-5 0.0 0.0 1.1847498992962585E-4 0.0 13 2.9618747482406463E-5 0.0 0.0 1.1847498992962585E-4 0.0 14 2.9618747482406463E-5 0.0 0.0 1.1847498992962585E-4 0.0 15 2.9618747482406463E-5 0.0 0.0 1.1847498992962585E-4 2.9618747482406463E-5 16 2.9618747482406463E-5 0.0 0.0 1.1847498992962585E-4 2.9618747482406463E-5 17 2.9618747482406463E-5 0.0 0.0 1.1847498992962585E-4 2.9618747482406463E-5 18 2.9618747482406463E-5 0.0 0.0 1.480937374120323E-4 2.9618747482406463E-5 19 2.9618747482406463E-5 0.0 0.0 2.0733123237684525E-4 2.9618747482406463E-5 20 2.9618747482406463E-5 0.0 0.0 2.369499798592517E-4 2.9618747482406463E-5 21 2.9618747482406463E-5 0.0 0.0 3.554249697888776E-4 2.9618747482406463E-5 22 2.9618747482406463E-5 0.0 0.0 4.4428121223609695E-4 2.9618747482406463E-5 23 2.9618747482406463E-5 0.0 0.0 5.331374546833163E-4 2.9618747482406463E-5 24 1.480937374120323E-4 0.0 0.0 6.516124446129423E-4 2.9618747482406463E-5 25 1.480937374120323E-4 0.0 0.0 7.997061820249745E-4 2.9618747482406463E-5 26 1.777124848944388E-4 0.0 0.0 9.181811719546004E-4 2.9618747482406463E-5 27 1.777124848944388E-4 0.0 0.0 0.0011847498992962585 2.9618747482406463E-5 28 2.0733123237684525E-4 0.0 0.0 0.0016882686064971684 2.9618747482406463E-5 29 2.0733123237684525E-4 0.0 0.0 0.003139587233135085 2.9618747482406463E-5 30 2.0733123237684525E-4 0.0 0.0 0.005124043314456319 2.9618747482406463E-5 31 2.0733123237684525E-4 0.0 0.0 0.010425799113807076 2.9618747482406463E-5 32 2.0733123237684525E-4 0.0 0.0 0.01732696727720778 2.9618747482406463E-5 33 2.369499798592517E-4 0.0 0.0 0.024879747885221428 2.9618747482406463E-5 34 2.369499798592517E-4 0.0 0.0 0.034890884534274816 2.9618747482406463E-5 35 2.6656872734165816E-4 0.0 0.0 0.04650143354737815 2.9618747482406463E-5 36 2.6656872734165816E-4 0.0 0.0 0.06347297585479705 2.9618747482406463E-5 37 2.6656872734165816E-4 0.0 0.0 0.08423571783996399 2.9618747482406463E-5 38 3.2580622230647113E-4 0.0 0.0 0.10736795962372343 2.9618747482406463E-5 39 3.2580622230647113E-4 0.0 0.0 0.13340283866075872 2.9618747482406463E-5 40 3.554249697888776E-4 0.0 0.0 0.15742364286899035 2.9618747482406463E-5 41 3.8504371727128404E-4 0.0 0.0 0.18221453451176456 2.9618747482406463E-5 42 3.8504371727128404E-4 0.0 0.0 0.20570220126531288 2.9618747482406463E-5 43 4.146624647536905E-4 0.0 0.0 0.23309954268653887 2.9618747482406463E-5 44 4.4428121223609695E-4 0.0 0.0 0.26511740871502026 2.9618747482406463E-5 45 4.4428121223609695E-4 0.0 0.0 0.30009714949174227 2.9618747482406463E-5 46 5.331374546833163E-4 0.0 0.0 0.34212615216927705 2.9618747482406463E-5 47 6.219936971305357E-4 0.0 0.0 0.38362201739212853 2.9618747482406463E-5 48 6.219936971305357E-4 0.0 0.0 0.42363694524085965 2.9618747482406463E-5 49 6.219936971305357E-4 0.0 0.0 0.4659917541407009 2.9618747482406463E-5 50 6.219936971305357E-4 0.0 0.0 0.5096794066772504 2.9618747482406463E-5 51 6.219936971305357E-4 0.0 0.0 0.5521823093145037 2.9618747482406463E-5 52 6.219936971305357E-4 0.0 0.0 0.5940632182546265 2.9618747482406463E-5 53 6.219936971305357E-4 0.0 0.0 0.639498376892638 2.9618747482406463E-5 54 6.516124446129423E-4 0.0 0.0 0.6905610975523067 2.9618747482406463E-5 55 6.516124446129423E-4 0.0 0.0 0.7448522616875578 2.9618747482406463E-5 56 6.812311920953487E-4 0.0 0.0 0.8051264128142549 2.9618747482406463E-5 57 6.812311920953487E-4 0.0 0.0 0.870494988507926 2.9618747482406463E-5 58 6.812311920953487E-4 2.9618747482406463E-5 0.0 0.9357450892116674 2.9618747482406463E-5 59 6.812311920953487E-4 2.9618747482406463E-5 0.0 1.005823045755041 2.9618747482406463E-5 60 6.812311920953487E-4 2.9618747482406463E-5 0.0 1.0785074520768665 2.9618747482406463E-5 61 6.812311920953487E-4 2.9618747482406463E-5 0.0 1.148940833590029 2.9618747482406463E-5 62 6.812311920953487E-4 2.9618747482406463E-5 0.0 1.2206774399924176 2.9618747482406463E-5 63 6.812311920953487E-4 2.9618747482406463E-5 0.0 1.2933026088192783 2.9618747482406463E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3870 0.0 39.87012 1 GTATCAA 8090 0.0 35.750443 1 ATCAACG 11355 0.0 25.462488 3 TATCAAC 11390 0.0 25.289019 2 TCAACGC 11480 0.0 25.216116 4 CAACGCA 11725 0.0 24.736544 5 AACGCAG 12155 0.0 23.858622 6 GTGGTAT 1900 0.0 21.303743 1 ACGCAGA 14440 0.0 19.962189 7 CGCAGAG 14660 0.0 19.616104 8 GTACATG 10160 0.0 19.545277 1 GAGTACT 10055 0.0 19.330976 12 TGGTATC 2000 0.0 19.191269 2 AGAGTAC 14405 0.0 18.790152 11 TACGCTA 445 0.0 18.600374 31 TACATGG 10335 0.0 18.502285 2 AGTACTT 10570 0.0 18.414406 13 GGACCGT 340 0.0 18.277409 6 CAGAGTA 15220 0.0 18.056181 10 GCAGAGT 15510 0.0 18.007414 9 >>END_MODULE