FastQCFastQC Report
Mon 6 Feb 2017
SRR2077491_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077491_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3157126
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26700.040.4812431
GTATCAA54050.033.733311
TCAACGC69750.025.523494
ATCAACG70500.025.2511633
TATCAAC71800.025.0818752
CAACGCA71850.024.7790745
AACGCAG73700.024.324656
GCGATAC2850.021.78057114
CGAATCG2750.021.33209637
GTACATG89300.021.0362431
ACGCAGA86750.020.428617
TGCGATA3150.019.7059213
TACATGG93950.019.6458532
CGCAGAG89700.019.604578
AGAGTAC86900.018.84868411
ACGTCGT1301.1849872E-418.57051323
ACATGGG97150.018.5373863
TATAGCG950.00783292118.1596955
CAGAGTA94950.017.57696210
GTGGTAT14100.017.1435071
Produced by FastQC (version 0.11.5)