FastQCFastQC Report
Mon 6 Feb 2017
SRR2077491_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077491_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3157126
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36300.036.593681
GTATCAA67450.032.6179541
TATCAAC92750.023.8530582
TCAACGC92850.023.6077354
ATCAACG93200.023.5194513
CAACGCA95000.023.007835
AACGCAG97550.022.3692266
GTACGCT3400.021.30128960
CGTGCCG4500.020.71827910
GTACATG86200.020.7073231
GTGCCGA4850.020.64708511
TACATGG88950.019.5478972
GCACCGT7650.019.4074346
CGCGTAA900.00570740419.18359410
ACGCAGA114450.018.9750187
GTGGTAT18800.018.9522551
GTATACG1501.6568125E-518.4493141
CGCAGAG118850.018.2440648
ACATGGG93100.018.2341563
AGAGTAC113400.018.1787411
Produced by FastQC (version 0.11.5)