FastQCFastQC Report
Mon 6 Feb 2017
SRR2077490_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077490_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3433113
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29050.035.5391431
GTATCAA58200.034.6474841
TCAACGC75050.026.2029864
CAACGCA75000.026.1280755
ATCAACG76450.025.8130193
TATCAAC77250.025.7671242
AACGCAG79250.024.7918686
CGTGCCG6650.021.257710
ACGCAGA93350.020.8277727
GTACATG93850.020.750411
CGCAGAG96600.020.094298
TACATGG94700.019.7076972
AGAGTAC92600.019.62390111
GTGCCGA7500.019.30962411
ACATGGG95550.018.9199893
GCACCGT9000.018.7666726
GTACGCT5050.018.4523360
GCGATAC5150.018.08056414
CAGAGTA102300.018.06521810
GAGTACT53500.018.04690412
Produced by FastQC (version 0.11.5)