FastQCFastQC Report
Mon 6 Feb 2017
SRR2077490_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077490_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3433113
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32800.038.9109341
GTATCAA64900.033.6815071
TATCAAC90150.024.1571962
ATCAACG89700.024.0124843
TCAACGC90000.023.855964
CAACGCA90650.023.6901765
AACGCAG95350.022.6313956
CGTGCCG6600.021.97170310
TATAGCG1103.250429E-521.971065
GTACATG83050.020.9065911
TACATGG86100.019.7530292
GCACCGT9000.019.5649916
ACGCAGA111800.019.2688967
GTGCCGA7600.019.0806911
CGCAGAG114950.018.5912388
GAGTACT75100.018.43583312
AGAGTAC110500.018.4352111
CCGATCG1702.4187138E-618.2776479
ACATGGG90700.018.1443393
CGAATCG5350.018.05017737
Produced by FastQC (version 0.11.5)