##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077490_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3433113 Sequences flagged as poor quality 0 Sequence length 75 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.75509486579673 31.0 31.0 34.0 27.0 34.0 2 30.850953930150276 33.0 31.0 34.0 27.0 34.0 3 30.961638023566366 33.0 31.0 34.0 27.0 34.0 4 34.33870309541224 37.0 35.0 37.0 31.0 37.0 5 34.00864463243709 37.0 35.0 37.0 30.0 37.0 6 33.97826637224001 37.0 35.0 37.0 30.0 37.0 7 33.82392015642946 37.0 35.0 37.0 29.0 37.0 8 33.7684343626324 37.0 35.0 37.0 28.0 37.0 9 35.24783687574513 39.0 35.0 39.0 28.0 39.0 10 35.12906740908324 39.0 35.0 39.0 28.0 39.0 11 35.12600488244925 39.0 35.0 39.0 27.0 39.0 12 35.01108760474823 39.0 35.0 39.0 27.0 39.0 13 34.954465233157194 39.0 35.0 39.0 27.0 39.0 14 36.198918590795 40.0 37.0 41.0 27.0 41.0 15 36.24256585786719 40.0 37.0 41.0 27.0 41.0 16 36.21278705361577 40.0 37.0 41.0 27.0 41.0 17 36.20196946619584 40.0 37.0 41.0 27.0 41.0 18 36.20267786117148 40.0 37.0 41.0 27.0 41.0 19 36.2306492678802 40.0 37.0 41.0 27.0 41.0 20 36.1921075711752 40.0 37.0 41.0 27.0 41.0 21 36.16412043530172 40.0 37.0 41.0 27.0 41.0 22 36.14354290115123 40.0 37.0 41.0 27.0 41.0 23 36.112236911514415 40.0 37.0 41.0 27.0 41.0 24 36.0949027893926 40.0 36.0 41.0 27.0 41.0 25 36.06586325588467 40.0 36.0 41.0 27.0 41.0 26 35.9960857682226 40.0 36.0 41.0 27.0 41.0 27 35.94208375896744 40.0 36.0 41.0 27.0 41.0 28 35.917104388932145 40.0 36.0 41.0 27.0 41.0 29 35.86617888779076 40.0 36.0 41.0 27.0 41.0 30 35.79439971827318 40.0 36.0 41.0 26.0 41.0 31 35.71596507309838 40.0 36.0 41.0 26.0 41.0 32 35.65187018312535 40.0 36.0 41.0 26.0 41.0 33 35.59575522273808 40.0 35.0 41.0 25.0 41.0 34 35.53464537869858 40.0 35.0 41.0 25.0 41.0 35 35.453888060194934 40.0 35.0 41.0 25.0 41.0 36 35.40333802004187 40.0 35.0 41.0 25.0 41.0 37 35.3487907330752 40.0 35.0 41.0 25.0 41.0 38 35.292419154277766 40.0 35.0 41.0 24.0 41.0 39 35.21473863516872 40.0 35.0 41.0 24.0 41.0 40 35.15553638927702 40.0 35.0 41.0 24.0 41.0 41 35.10963286090496 40.0 35.0 41.0 24.0 41.0 42 35.04775374419659 40.0 35.0 41.0 24.0 41.0 43 34.95389898322601 40.0 35.0 41.0 23.0 41.0 44 34.82924447869907 39.0 35.0 41.0 23.0 41.0 45 34.7421319950727 39.0 35.0 41.0 22.0 41.0 46 34.62295153116137 39.0 35.0 41.0 21.0 41.0 47 34.485314348814036 39.0 34.0 41.0 19.0 41.0 48 34.38871484859368 39.0 34.0 41.0 19.0 41.0 49 34.29727364057052 39.0 34.0 41.0 18.0 41.0 50 34.29472347691439 39.0 34.0 41.0 17.0 41.0 51 34.21705315263436 39.0 34.0 41.0 16.0 41.0 52 34.14376485714278 39.0 34.0 41.0 15.0 41.0 53 34.04441420949441 39.0 34.0 41.0 15.0 41.0 54 34.00097724718062 39.0 34.0 41.0 14.0 41.0 55 33.91066591749237 39.0 34.0 41.0 13.0 41.0 56 33.77582852647146 39.0 34.0 41.0 13.0 41.0 57 33.692990880288534 38.0 34.0 41.0 11.0 41.0 58 33.583842710682696 38.0 34.0 41.0 9.0 41.0 59 33.48368492385774 38.0 34.0 40.0 8.0 41.0 60 33.44501069437563 38.0 34.0 40.0 8.0 41.0 61 33.322199997494984 37.0 34.0 40.0 7.0 41.0 62 33.16727209386932 37.0 33.0 40.0 2.0 41.0 63 33.02360306811923 37.0 33.0 40.0 2.0 41.0 64 32.86040861457226 37.0 33.0 40.0 2.0 41.0 65 32.72579580107034 36.0 33.0 40.0 2.0 41.0 66 32.520857600667384 36.0 33.0 39.0 2.0 41.0 67 32.32373999923684 36.0 33.0 39.0 2.0 41.0 68 32.108640175840414 36.0 33.0 39.0 2.0 41.0 69 31.947367593201854 35.0 33.0 39.0 2.0 41.0 70 31.757637747432142 35.0 32.0 39.0 2.0 41.0 71 31.577191312957073 35.0 32.0 39.0 2.0 41.0 72 31.381007849144492 35.0 32.0 38.0 2.0 40.0 73 31.188418499478463 35.0 32.0 37.0 2.0 40.0 74 30.93564383112353 35.0 32.0 37.0 2.0 39.0 75 30.722923189536726 35.0 32.0 37.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5620981208656808 1101 2 0.5362586194488017 1101 3 0.5777256630760306 1101 4 0.5086499143640069 1101 5 0.6277930613363125 1101 6 0.6414774196408715 1101 7 0.644728680924807 1101 8 0.7567256001276945 1101 9 0.8866786062849457 1101 10 0.8686333887054474 1101 11 0.8753192039099105 1101 12 0.99056440473516 1101 13 0.8981336825611379 1101 14 1.0388430781774787 1101 15 1.0135440747000146 1101 16 1.0182933028720953 1101 17 1.091165461619802 1101 18 0.9943630363722065 1101 19 1.0341606461334223 1101 20 1.0812947772643184 1101 21 1.1234631274431663 1101 22 1.1226296986412834 1101 23 1.021632143290276 1101 24 1.0208459932597194 1101 25 1.1137311608847895 1101 26 1.086248270514261 1101 27 1.0815867583359946 1101 28 1.086307697879164 1101 29 1.0684698748683985 1101 30 1.072318576036544 1101 31 1.1182219571040548 1101 32 1.1031517893523812 1101 33 1.153548949969462 1101 34 1.095387624393581 1101 35 1.2164695961570544 1101 36 1.1299570922772801 1101 37 1.1912118135578993 1101 38 1.214220463444498 1101 39 1.2396281981137136 1101 40 1.146869492557407 1101 41 1.3189766732022932 1101 42 1.16050098450377 1101 43 1.2050048826598143 1101 44 1.2517087296496072 1101 45 1.198036316303373 1101 46 1.1913064158500646 1101 47 1.1828502988621423 1101 48 1.1863023784893088 1101 49 1.2272670850382923 1101 50 1.062683949887088 1101 51 1.165148034893555 1101 52 1.0690269942500237 1101 53 1.0670478676806496 1101 54 1.1587091991757603 1101 55 1.1448220262508357 1101 56 1.1372985480945346 1101 57 1.1127356873242036 1101 58 1.1319750527010228 1101 59 1.1005952220239976 1101 60 1.0607654273988132 1101 61 1.0577279847570082 1101 62 1.1086436448142507 1101 63 1.1633282714277229 1101 64 1.2013728277777176 1101 65 1.1428249772135572 1101 66 1.1874392792057549 1101 67 1.1997223163752508 1101 68 1.2508240015314556 1101 69 1.1808211464845257 1101 70 1.2106339418965781 1101 71 1.1814704522500996 1101 72 1.2132559235068143 1101 73 1.1982325010983352 1101 74 1.217044304377783 1101 75 1.2472199945774491 1102 1 0.6999626095425739 1102 2 0.6923155806148067 1102 3 0.7007767095821293 1102 4 0.7043548078456823 1102 5 0.7387963136411457 1102 6 0.7558920082163851 1102 7 0.8076382945559573 1102 8 0.8575019940046218 1102 9 1.0148597518764149 1102 10 0.9798629549752107 1102 11 1.0078855163999592 1102 12 1.0454694476340194 1102 13 1.005929772214202 1102 14 1.177104628546168 1102 15 1.0447760910532011 1102 16 1.1581360357730262 1102 17 1.1319359180934327 1102 18 1.059221844715033 1102 19 1.1278143959326954 1102 20 1.1455627743504095 1102 21 1.1637474439457023 1102 22 1.2146678923749974 1102 23 1.0613620035011238 1102 24 1.1849077663757939 1102 25 1.1697224591257651 1102 26 1.188675800078947 1102 27 1.193003957577055 1102 28 1.2484529957521104 1102 29 1.1651791647163705 1102 30 1.1915085874151288 1102 31 1.2729866801757126 1102 32 1.2473883040725227 1102 33 1.2842673619845897 1102 34 1.2510793129249151 1102 35 1.3692349684491205 1102 36 1.302715024723902 1102 37 1.3059468771808085 1102 38 1.359062262488031 1102 39 1.3304572015879401 1102 40 1.2958862544361764 1102 41 1.3278278149549507 1102 42 1.2758603714246703 1102 43 1.2905283872646578 1102 44 1.2566490420335228 1102 45 1.350136185976858 1102 46 1.259941746949032 1102 47 1.414768011207876 1102 48 1.382799586221232 1102 49 1.3638672479636682 1102 50 1.354152990274521 1102 51 1.313434854547431 1102 52 1.355103955815764 1102 53 1.2925089554625586 1102 54 1.334093390176129 1102 55 1.3978301842360779 1102 56 1.34108018811353 1102 57 1.3416336976910586 1102 58 1.3344383514161606 1102 59 1.379895514724538 1102 60 1.2894642608007203 1102 61 1.3300171966020429 1102 62 1.3562280210698745 1102 63 1.3707508353521547 1102 64 1.3416001307355288 1102 65 1.2808315259316245 1102 66 1.3619487111654536 1102 67 1.3446841212631497 1102 68 1.3741706259643394 1102 69 1.3472459923291353 1102 70 1.3363071117945289 1102 71 1.3460207775150472 1102 72 1.3410962628381995 1102 73 1.3878543991238637 1102 74 1.476136233162027 1102 75 1.4725564244578564 1103 1 0.5754520706116679 1103 2 0.5556643704433277 1103 3 0.5681385790777149 1103 4 0.578718088178853 1103 5 0.6174765662470278 1103 6 0.7413489689411747 1103 7 0.8005626886619908 1103 8 0.8262258681310115 1103 9 0.9407241000364763 1103 10 1.023776163893615 1103 11 0.9615946711323744 1103 12 0.991843637065152 1103 13 0.9394257850785621 1103 14 1.0385735678122572 1103 15 1.0304619411022458 1103 16 1.0008585191058117 1103 17 1.067042400122709 1103 18 1.022248944823076 1103 19 1.0930992726201865 1103 20 1.052798263316845 1103 21 1.103098642931343 1103 22 1.0989240893594783 1103 23 1.0980567931654122 1103 24 1.1646831477125232 1103 25 1.2170458118500278 1103 26 1.2698287847892686 1103 27 1.22229653761881 1103 28 1.2073821525665096 1103 29 1.0968392944591159 1103 30 1.1489388909391849 1103 31 1.1970257329618548 1103 32 1.1925001268526998 1103 33 1.2501559827583009 1103 34 1.2222298018363915 1103 35 1.2537715386913106 1103 36 1.1879924948373528 1103 37 1.2498746503139841 1103 38 1.190083587343409 1103 39 1.259633251747026 1103 40 1.2286125137962287 1103 41 1.2340412225673987 1103 42 1.2771751110566 1103 43 1.2457979012110698 1103 44 1.2454404158802674 1103 45 1.2239276471118998 1103 46 1.1773260366065301 1103 47 1.2347763811861299 1103 48 1.2586557389796411 1103 49 1.3125026893418124 1103 50 1.2462674782575078 1103 51 1.2711165968736537 1103 52 1.3113173149347048 1103 53 1.2610935724188579 1103 54 1.363276859840859 1103 55 1.3534376170612603 1103 56 1.3091127873845778 1103 57 1.22556232107695 1103 58 1.2835179932625564 1103 59 1.2647611941928432 1103 60 1.204073105756649 1103 61 1.2657967684238258 1103 62 1.266128874490832 1103 63 1.220261519763561 1103 64 1.2912850522956703 1103 65 1.2237727796114726 1103 66 1.3168228414345151 1103 67 1.2843327470543215 1103 68 1.2832576776176765 1103 69 1.2697115926560443 1103 70 1.2949010168727035 1103 71 1.2797287069783359 1103 72 1.275117217849811 1103 73 1.2775715300673092 1103 74 1.2680096437875328 1103 75 1.3498260819450252 1104 1 0.5437622272997018 1104 2 0.5742493369260089 1104 3 0.5623433673460241 1104 4 0.6529895609474039 1104 5 0.6328388842756993 1104 6 0.7061580026274896 1104 7 0.6928558296914957 1104 8 0.7299289960561026 1104 9 0.8338669035585582 1104 10 0.8657454761385139 1104 11 0.9378239623597935 1104 12 0.9281051781194876 1104 13 0.9150827212422996 1104 14 1.0148269016871367 1104 15 1.005608846346675 1104 16 0.9580770789092128 1104 17 0.987938822063235 1104 18 0.8907975205413052 1104 19 0.943904607940425 1104 20 0.9909939536606203 1104 21 1.023460291033821 1104 22 1.0538266185993024 1104 23 1.0632736181634215 1104 24 1.095987701763427 1104 25 1.1046539045584822 1104 26 1.1600486559673655 1104 27 1.1638754719646442 1104 28 1.145232609166733 1104 29 1.0403565797220935 1104 30 1.0459586297193226 1104 31 1.098057325934306 1104 32 1.061893229740761 1104 33 1.1078267777576158 1104 34 1.181653282492796 1104 35 1.1921481324503986 1104 36 1.2451257615982314 1104 37 1.1429980796638475 1104 38 1.1607198118167688 1104 39 1.2305554203284714 1104 40 1.2117441456570788 1104 41 1.1446726011634638 1104 42 1.193134620437199 1104 43 1.2388701173876129 1104 44 1.220820978310151 1104 45 1.206458212786373 1104 46 1.3479284613191993 1104 47 1.2814170164348937 1104 48 1.2625674543781855 1104 49 1.1958799763694756 1104 50 1.2056792877258289 1104 51 1.179006880236095 1104 52 1.0730128958027336 1104 53 1.1406385081269548 1104 54 1.138258687801013 1104 55 1.1010920583918349 1104 56 1.238437441088756 1104 57 1.2754134667441122 1104 58 1.219352135409224 1104 59 1.2067038027593995 1104 60 1.2246076274970363 1104 61 1.1839205829228945 1104 62 1.2559973495380703 1104 63 1.1447736757728038 1104 64 1.2056834235580354 1104 65 1.2131179185849774 1104 66 1.267805952291667 1104 67 1.2706377749554605 1104 68 1.251471897139652 1104 69 1.18446220037481 1104 70 1.1859318021989402 1104 71 1.1804008273577757 1104 72 1.2348089024084032 1104 73 1.187800486098208 1104 74 1.1966064822027356 1104 75 1.251859763126447 1105 1 0.3043957026088755 1105 2 0.32361721466708104 1105 3 0.3494681839268807 1105 4 0.3533002638921374 1105 5 0.3981737747877929 1105 6 0.4363786329192436 1105 7 0.47163305907218955 1105 8 0.44063491734695504 1105 9 0.5240417416741394 1105 10 0.5594046641618036 1105 11 0.5782674047462564 1105 12 0.5376784127761027 1105 13 0.5606353825848487 1105 14 0.6106752887432094 1105 15 0.5467339435098069 1105 16 0.565313865497231 1105 17 0.5478887312517315 1105 18 0.5880214784603126 1105 19 0.6396419859496518 1105 20 0.6534312239924276 1105 21 0.6814758218748693 1105 22 0.6986582952959921 1105 23 0.6044386420185788 1105 24 0.6634359343708169 1105 25 0.601529725898132 1105 26 0.6591150059704134 1105 27 0.7113602074652334 1105 28 0.6335853471745807 1105 29 0.6124686428033996 1105 30 0.6181166254945722 1105 31 0.6534112082554984 1105 32 0.6927685444609253 1105 33 0.6281915060117171 1105 34 0.6646516509000477 1105 35 0.621105439900056 1105 36 0.6406757976757973 1105 37 0.6651389979958964 1105 38 0.6285158561505853 1105 39 0.6846801800222906 1105 40 0.6371492995924157 1105 41 0.5831777140842362 1105 42 0.5875567369084607 1105 43 0.6547525475202463 1105 44 0.7555098322811276 1105 45 0.6793611341132646 1105 46 0.6953507041809104 1105 47 0.7237712211159604 1105 48 0.6530774727293576 1105 49 0.6835040239176706 1105 50 0.5933018545490256 1105 51 0.657600620592028 1105 52 0.5765852204178472 1105 53 0.5796695428387011 1105 54 0.6704387327802976 1105 55 0.64041131320338 1105 56 0.5592408567889251 1105 57 0.5838387391238555 1105 58 0.5632707638287613 1105 59 0.5672658557513728 1105 60 0.6042640449025356 1105 61 0.5583186134377698 1105 62 0.5595148495544962 1105 63 0.5564699225655403 1105 64 0.6101939612206166 1105 65 0.5942042177311677 1105 66 0.5188043651206655 1105 67 0.5775945232047874 1105 68 0.6211552161454605 1105 69 0.5682078362967147 1105 70 0.6256668989850915 1105 71 0.6158184020072746 1105 72 0.6230105762471467 1105 73 0.688853085152104 1105 74 0.6963261265658325 1105 75 0.6571183313303415 1106 1 0.3023557654107236 1106 2 0.34322540235632104 1106 3 0.3962583388264882 1106 4 0.40462523691596886 1106 5 0.3585531670144704 1106 6 0.4195534438162838 1106 7 0.46668926917620723 1106 8 0.43502491688249734 1106 9 0.49814527576918266 1106 10 0.5206423575493488 1106 11 0.46644020213724247 1106 12 0.48537566983618774 1106 13 0.4969253604140462 1106 14 0.531437026192954 1106 15 0.4858286116888948 1106 16 0.452231165484406 1106 17 0.5736214334556067 1106 18 0.5844002299232329 1106 19 0.5877964105991325 1106 20 0.6058769721624984 1106 21 0.6161826655278517 1106 22 0.5774491314992645 1106 23 0.567951345699683 1106 24 0.6083769885526138 1106 25 0.5923708435365356 1106 26 0.589161255726971 1106 27 0.65423697134284 1106 28 0.5953099203991528 1106 29 0.5550631950135738 1106 30 0.5322323711411059 1106 31 0.5918657324391887 1106 32 0.5982398488825567 1106 33 0.5930976229651037 1106 34 0.6115752958192218 1106 35 0.6589319747673272 1106 36 0.6992363045353898 1106 37 0.6100663808981039 1106 38 0.47889901290080417 1106 39 0.600780412156972 1106 40 0.5934951539464066 1106 41 0.5792455624209651 1106 42 0.6001204970221963 1106 43 0.6345053095379782 1106 44 0.6387734007709227 1106 45 0.6555719045587765 1106 46 0.6870018542490541 1106 47 0.7452296615561735 1106 48 0.6154784644111686 1106 49 0.6421664857119112 1106 50 0.6267167950920793 1106 51 0.6014184788996744 1106 52 0.5565882944506342 1106 53 0.5791956051463387 1106 54 0.5523941238689432 1106 55 0.5912802342142243 1106 56 0.6261019813059931 1106 57 0.6030825416411645 1106 58 0.6634294017114186 1106 59 0.6485686273462932 1106 60 0.621039215350585 1106 61 0.5327147493727225 1106 62 0.566789173367539 1106 63 0.5167274398917883 1106 64 0.5173938622332557 1106 65 0.5533799540462354 1106 66 0.5808727035818819 1106 67 0.5470236827535757 1106 68 0.5659535786251126 1106 69 0.4985687442242508 1106 70 0.5022527218462862 1106 71 0.5613596800480742 1106 72 0.5716441154468583 1106 73 0.520229189490724 1106 74 0.6089499163568206 1106 75 0.6451660966667276 1107 1 0.21436839074889136 1107 2 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2214 15 -1.288390173109697 2214 16 -1.2784899080728351 2214 17 -1.2310332257546506 2214 18 -1.232697000520652 2214 19 -1.1735036533737855 2214 20 -1.2309214640837425 2214 21 -1.2946813045501244 2214 22 -1.239685540208633 2214 23 -1.1699473472774358 2214 24 -1.1842738077520636 2214 25 -1.278099296087099 2214 26 -1.2617344619607351 2214 27 -1.3008573967809625 2214 28 -1.4393738590769658 2214 29 -1.3049342357410652 2214 30 -1.3462595592981899 2214 31 -1.277958416272348 2214 32 -1.284390503200889 2214 33 -1.2494343618802262 2214 34 -1.2594217973185025 2214 35 -1.207633686558367 2214 36 -1.2889298029868854 2214 37 -1.2820734712605457 2214 38 -1.2549091574285427 2214 39 -1.3179253724537645 2214 40 -1.3000510049995029 2214 41 -1.378625075429703 2214 42 -1.3888820354274358 2214 43 -1.3833299656843607 2214 44 -1.3223726375882947 2214 45 -1.267210846380003 2214 46 -1.2492491648956943 2214 47 -1.3712286789049841 2214 48 -1.2851146568539846 2214 49 -1.2605194085500813 2214 50 -1.2334001249378943 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-1.2923286355000982 2215 13 -1.359293161447738 2215 14 -1.590445012020389 2215 15 -1.6457442332538221 2215 16 -1.5119199370257235 2215 17 -1.526511829426049 2215 18 -1.5763376287739561 2215 19 -1.5259470287302648 2215 20 -1.4994431634793983 2215 21 -1.4643671397186466 2215 22 -1.4987153967187155 2215 23 -1.424554421720039 2215 24 -1.6417138249963443 2215 25 -1.5428551523734058 2215 26 -1.5750940806835843 2215 27 -1.5006271891594736 2215 28 -1.4058069507104065 2215 29 -1.573281612845463 2215 30 -1.5698021136424885 2215 31 -1.5331627560046428 2215 32 -1.5760785955141756 2215 33 -1.6341306795861215 2215 34 -1.642219129741072 2215 35 -1.622944299455689 2215 36 -1.7339217226670698 2215 37 -1.6774406273080658 2215 38 -1.6615519056828916 2215 39 -1.6492023918949883 2215 40 -1.6743648385716412 2215 41 -1.6483391028825238 2215 42 -1.6536529275987064 2215 43 -1.705816219743241 2215 44 -1.6195563458545408 2215 45 -1.6696356764994604 2215 46 -1.6296260428762608 2215 47 -1.641360333935971 2215 48 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-1.1586268585500719 2216 10 -1.4863153768035104 2216 11 -1.5192267091963103 2216 12 -1.2761471699286417 2216 13 -1.2967211592552275 2216 14 -1.4725153461690397 2216 15 -1.3377192553663164 2216 16 -1.357310906560926 2216 17 -1.6372706652676356 2216 18 -1.3414570506982315 2216 19 -1.6573339707598578 2216 20 -1.6983230578084019 2216 21 -1.711168387541953 2216 22 -1.8295303015289832 2216 23 -1.804842078774449 2216 24 -1.8473779805872255 2216 25 -1.831987414957048 2216 26 -1.9478417944959148 2216 27 -1.7527964856602196 2216 28 -1.7289182414907813 2216 29 -1.7657452301811958 2216 30 -1.7121985938231674 2216 31 -1.7352785787058167 2216 32 -1.6879826058103262 2216 33 -1.6396484462180396 2216 34 -1.7779079734521162 2216 35 -1.7012307525716395 2216 36 -1.8147555231453154 2216 37 -1.814305272232339 2216 38 -1.9100681651482816 2216 39 -1.7893603397053113 2216 40 -1.7402059433002464 2216 41 -1.679045033748551 2216 42 -1.6670417712084316 2216 43 -1.6485336440386291 2216 44 -1.6812457589685508 2216 45 -1.7236348784089728 2216 46 -1.6024412629279254 2216 47 -1.8171969503101977 2216 48 -1.7910959160709723 2216 49 -1.7869836484412573 2216 50 -1.6305706986759105 2216 51 -1.7645329587012029 2216 52 -1.708157721281225 2216 53 -1.7204788829065052 2216 54 -1.760542390961085 2216 55 -1.652532445766262 2216 56 -1.9336991862863684 2216 57 -1.798814819806271 2216 58 -1.7340391710025251 2216 59 -1.8040416883058015 2216 60 -1.795489607458098 2216 61 -1.6892873893149023 2216 62 -1.7009767761929027 2216 63 -1.7087490639743592 2216 64 -1.6755345649086664 2216 65 -1.609010450939497 2216 66 -1.7092863744343987 2216 67 -1.7300243365423356 2216 68 -1.7946682252347728 2216 69 -1.8668323042481063 2216 70 -1.729679392479646 2216 71 -1.663478621868311 2216 72 -1.7558038498887107 2216 73 -1.7738137605237334 2216 74 -1.8413154469894089 2216 75 -1.9123721727016445 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 86919.0 3 34225.0 4 19558.0 5 11858.0 6 8754.0 7 7721.0 8 7365.0 9 7594.0 10 8019.0 11 8558.0 12 8906.0 13 9465.0 14 9966.0 15 10357.0 16 10773.0 17 11447.0 18 11917.0 19 13089.0 20 14599.0 21 16780.0 22 20740.0 23 24908.0 24 27009.0 25 27878.0 26 29774.0 27 32378.0 28 37619.0 29 44255.0 30 51683.0 31 59337.0 32 72240.0 33 90550.0 34 116455.0 35 159816.0 36 228229.0 37 366257.0 38 618776.0 39 894167.0 40 213172.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.85966928162073 17.699604127843884 13.973374748189338 26.467351842346048 2 15.504731884174081 18.99328927492997 37.592092210926815 27.909886629969133 3 18.44097442812164 22.854744308888737 28.819739070074853 29.884542192914772 4 12.764101838631534 15.663005543458763 35.06721133834933 36.50568127956038 5 14.50293458147192 36.680417065966644 33.64140291974165 15.17524543281979 6 34.35087269293101 35.31220487588756 16.19416689013513 14.142755541046299 7 29.58802523897543 31.093319876275878 20.118864199487668 19.199790685261025 8 27.16201282364674 34.65067988869119 18.529636364219073 19.657670923443 9 26.294795058355135 15.667717425292635 18.281801282183917 39.755686234168316 10 14.866900088895468 28.83799842721163 32.548619352289734 23.74648213160317 11 35.953632612156724 22.45316576282889 22.18307776792126 19.410123857093122 12 23.9500512596819 24.138965639101514 29.272427422938446 22.63855567827814 13 28.616265750954245 21.143820355088305 25.19388873324473 25.04602516071272 14 22.425424697901615 20.520309206066855 26.250197435638896 30.80406866039263 15 24.171950333771992 28.369526805038873 23.116693027948397 24.341829833240737 16 24.111303289751255 27.54653857001998 24.585779920232913 23.75637821999585 17 22.885162222636584 27.52756153272422 25.933304586984896 23.6539716576543 18 22.849774459353593 26.729953423518516 26.940912516799376 23.479359600328515 19 23.759345620143655 26.468322775812325 27.092335193135046 22.679996410908974 20 23.75085902794306 25.818323346420367 27.357130434453293 23.07368719118328 21 23.931203573265194 25.585353808321592 27.355903215326816 23.1275394030864 22 23.67875194826004 25.327322253135424 27.251868144673786 23.74205765393075 23 23.577127059107998 25.42481460927019 27.14723323180724 23.850825099814575 24 23.280905619918745 25.567155982280337 27.17666784661798 23.975270551182938 25 23.60883794669987 25.409009363851258 27.23548766198542 23.74666502746345 26 23.307919491559996 25.903150011014027 27.322210916821387 23.466719580604593 27 23.45852752885399 25.81585427964388 27.143114377519588 23.582503813982544 28 23.538441365444292 25.74314145431764 27.064906682984397 23.65351049725367 29 23.516793004835638 25.765176244441406 27.13569596540653 23.582334785316426 30 23.51257147652493 25.76686473016665 27.258256077690003 23.462307715618422 31 23.595711846378357 25.32486431636102 27.119393153486172 23.960030683774452 32 23.318769583572696 25.83907124487725 27.105237706264763 23.736921465285295 33 23.52028484223645 25.611534222814704 27.016401676029243 23.8517792589196 34 23.763564592952964 25.637393850232325 26.98518637204492 23.613855184769783 35 23.57383845545701 25.69170662818398 27.051699357333742 23.682755559025264 36 23.618200096657173 25.70489383005059 26.89554237985802 23.781363693434216 37 23.558230381347165 25.485386692612217 27.047013688414516 23.9093692376261 38 23.683647113757043 25.650395409066114 26.947705334097282 23.718252143079564 39 23.846833263031314 25.816212267042026 26.774174378526265 23.56278009140039 40 23.670419011518053 25.520567203989398 27.140197011213917 23.66881677327863 41 23.46032726329926 25.64122806762004 27.004203675812988 23.894240993267708 42 23.8549433199383 25.564127922303964 26.94431619070368 23.63661256705405 43 23.63783185724698 25.51432884535497 26.891077237157873 23.95676206024017 44 23.73973025337795 25.814610153820073 26.661999891050307 23.783659701751674 45 23.67217389632566 25.7526925884562 26.613878125526153 23.961255389691992 46 23.78346336852561 25.62748230798023 26.466329453881322 24.122724869612835 47 23.58791260648332 25.785421907570555 26.4773668689039 24.149298617042227 48 23.520952104357217 25.978844132120326 26.52701024585329 23.973193517669166 49 23.837324969932013 25.762928798361106 26.416137472972306 23.983608758734572 50 23.930480465653996 25.805924487643413 26.39023485140428 23.873360195298314 51 23.63013329534965 26.10958817122835 26.1312889722355 24.1289895611865 52 23.799527063910457 26.049119405584936 26.052789641313108 24.098563889191503 53 23.62596279236949 26.092324954057734 26.234703023174593 24.047009230398185 54 23.594475334776337 26.0605170875529 26.381421176640558 23.963586401030202 55 23.756729699050684 25.78672996411701 26.274481132666278 24.182059204166027 56 23.507903016864063 26.21475802718653 26.234392127949086 24.042946828000325 57 23.712600560558297 25.897187479427654 26.195293570707424 24.19491838930663 58 23.652988944980063 25.826213676789582 26.29698339780857 24.22381398042178 59 23.626789293674147 25.968867258653088 26.36818572561741 24.036157722055354 60 23.58904465515353 26.003185492609514 26.485990357301816 23.921779494935137 61 23.68098724206463 25.874411861771897 26.519022719838368 23.925578176325104 62 23.415182733544658 26.087223801976506 26.467374836845046 24.030218627633786 63 23.33213801002848 25.919600282333228 26.59837107826416 24.14989062937413 64 23.67095352026062 25.79319220809714 26.40183083906951 24.134023432572725 65 23.667617669482773 25.809311359565008 26.394217421210147 24.128853549742068 66 23.618415015185615 25.904254156925294 26.313457294557214 24.163873533331877 67 23.607054355571634 25.898560427158152 26.251184980478047 24.243200236792166 68 23.650779685468887 25.913507731014196 26.45031169356319 23.98540088995373 69 23.614166111013528 25.89110596676801 26.258987671345807 24.23574025087266 70 23.423007430566763 25.635834058250097 26.495770817152593 24.44538769403055 71 23.497968316279074 25.85448256515618 26.317797922261484 24.329751196303263 72 23.32571503485044 26.02305882695683 26.273428452815345 24.377797685377384 73 23.510286906627975 25.824334760035295 26.303148483523504 24.362229849813232 74 23.485582567746228 25.94750326443972 26.15065309188036 24.416261075933694 75 23.591710733204664 25.96000193413118 26.157085241006143 24.29120209165801 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 721.0 1 508.5 2 383.5 3 500.0 4 529.0 5 414.5 6 359.5 7 752.0 8 1085.0 9 1809.0 10 2957.5 11 3372.5 12 3363.0 13 3135.0 14 2999.5 15 3435.0 16 3778.0 17 4124.5 18 4869.5 19 5798.0 20 6328.0 21 7434.5 22 9683.0 23 12353.0 24 13881.0 25 15875.0 26 20843.5 27 26328.5 28 28839.0 29 32981.5 30 41068.0 31 49565.0 32 54118.0 33 58526.5 34 68533.5 35 79858.5 36 85585.0 37 89835.0 38 99086.5 39 107754.5 40 111421.0 41 117376.0 42 127416.0 43 135458.0 44 139415.0 45 145077.5 46 154360.5 47 161756.5 48 165532.0 49 169776.0 50 175828.5 51 178278.5 52 178920.0 53 176520.5 54 172661.5 55 166179.0 56 161156.0 57 156234.5 58 142089.0 59 121363.0 60 109861.0 61 101090.5 62 81210.5 63 60939.5 64 51778.0 65 42979.5 66 28707.0 67 20505.5 68 17778.0 69 14680.5 70 9500.5 71 5989.0 72 4560.0 73 4072.0 74 3048.0 75 1945.0 76 1378.0 77 1120.5 78 672.0 79 371.5 80 262.0 81 199.5 82 96.0 83 45.5 84 36.0 85 24.0 86 11.5 87 7.5 88 4.0 89 3.5 90 4.5 91 5.0 92 4.0 93 2.0 94 0.0 95 1.0 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07165508388450947 2 0.01596218941817528 3 0.0024467589619100796 4 0.13830013751367928 5 0.09181171723738776 6 0.06853837901636212 7 0.027992087647566506 8 0.025050151276698435 9 0.04730400659692821 10 0.028895058216842847 11 0.015379627760577644 12 0.10244346748854466 13 0.08517051434077469 14 0.04704185385100928 15 0.019807096358319693 16 0.03265258090834761 17 0.02309856972374635 18 0.020214889518638042 19 0.014243632528262249 20 0.013224149627466384 21 0.027904703398926863 22 0.016399110661373513 23 0.014505785274181187 24 0.022370367651749302 25 0.016049573666814927 26 0.03276909323986714 27 0.029273723294281316 28 0.03678876867729084 29 0.04992553405611758 30 0.03544887686481628 31 0.019807096358319693 32 0.01133082424027406 33 0.013864967450823785 34 0.013486302373385321 35 0.006612074813733192 36 0.010544366002517248 37 0.008243247455006578 38 0.0025050151276698436 39 0.00888406527836398 40 0.01208815439515099 41 0.011738617400592406 42 0.007223764554210712 43 0.011272568074514298 44 0.009787035847640319 45 0.004980902172459806 46 0.0019807096358319693 47 0.005272183001258624 48 0.006175153570534964 49 0.0017476849727929144 50 1.7476849727929143E-4 51 0.0017476849727929144 52 0.0026506555420692532 53 0.0 54 0.0 55 2.91280828798819E-4 56 0.00902970569276339 57 0.0031167048681473635 58 3.4953699455858285E-4 59 0.007689813880288822 60 0.0014272760611142132 61 9.903548179159847E-4 62 0.02309856972374635 63 0.008447144035165752 64 0.010777390665556304 65 0.017360337396409613 66 0.017127312733370557 67 0.01651562299289304 68 0.016748647655932095 69 0.006291665902054491 70 0.015583524340736819 71 0.007165508388450948 72 0.017127312733370557 73 0.007398533051490004 74 0.019544943612400757 75 0.0013690198953544494 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 3433113.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.079585293315894 #Duplication Level Percentage of deduplicated Percentage of total 1 76.59669608960576 45.253010398120594 2 12.86263887191896 15.198387406613136 3 4.52151358934078 8.013874432690367 4 2.125484464035373 5.022909627303826 5 1.1424214981076783 3.3746894169185144 6 0.6880609063604646 2.439021180259158 7 0.447452194731574 1.850470307232779 8 0.293353986490279 1.3864985492789341 9 0.21394360547479885 1.1375729538847115 >10 1.0194529536083667 10.450717344115724 >50 0.059071042948711495 2.376637524908292 >100 0.028918914532338108 2.9401259021316997 >500 5.455355663977534E-4 0.23425360874019144 >1k 4.4634727849876175E-4 0.32183134780201006 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.9128082879881902E-5 0.0 2 0.0 0.0 0.0 2.9128082879881902E-5 0.0 3 0.0 0.0 0.0 2.9128082879881902E-5 0.0 4 0.0 0.0 0.0 2.9128082879881902E-5 0.0 5 0.0 0.0 0.0 2.9128082879881902E-5 0.0 6 0.0 0.0 0.0 2.9128082879881902E-5 0.0 7 0.0 0.0 0.0 2.9128082879881902E-5 0.0 8 0.0 0.0 0.0 2.9128082879881902E-5 0.0 9 0.0 0.0 0.0 2.9128082879881902E-5 0.0 10 0.0 0.0 0.0 5.8256165759763804E-5 0.0 11 0.0 0.0 0.0 8.738424863964571E-5 0.0 12 0.0 0.0 0.0 8.738424863964571E-5 0.0 13 0.0 0.0 0.0 1.1651233151952761E-4 2.9128082879881902E-5 14 0.0 0.0 0.0 1.456404143994095E-4 2.9128082879881902E-5 15 0.0 0.0 0.0 1.7476849727929143E-4 5.8256165759763804E-5 16 0.0 0.0 0.0 1.7476849727929143E-4 1.1651233151952761E-4 17 0.0 0.0 0.0 1.7476849727929143E-4 1.1651233151952761E-4 18 0.0 0.0 0.0 1.7476849727929143E-4 1.1651233151952761E-4 19 0.0 0.0 0.0 1.7476849727929143E-4 1.1651233151952761E-4 20 0.0 0.0 0.0 2.0389658015917332E-4 1.1651233151952761E-4 21 0.0 0.0 0.0 2.0389658015917332E-4 1.456404143994095E-4 22 0.0 0.0 0.0 2.0389658015917332E-4 1.7476849727929143E-4 23 0.0 0.0 0.0 2.621527459189371E-4 1.7476849727929143E-4 24 0.0 0.0 0.0 3.4953699455858285E-4 1.7476849727929143E-4 25 0.0 0.0 0.0 3.4953699455858285E-4 1.7476849727929143E-4 26 0.0 0.0 0.0 5.243054918378742E-4 1.7476849727929143E-4 27 0.0 0.0 0.0 6.408178233574019E-4 1.7476849727929143E-4 28 0.0 0.0 0.0 0.0011068671494355122 1.7476849727929143E-4 29 0.0 0.0 0.0 0.0022137342988710245 1.7476849727929143E-4 30 0.0 0.0 0.0 0.0034371137798260647 1.7476849727929143E-4 31 0.0 0.0 0.0 0.007311148802850357 1.7476849727929143E-4 32 0.0 0.0 0.0 0.012000770146511344 1.7476849727929143E-4 33 0.0 0.0 0.0 0.017564233976568786 2.0389658015917332E-4 34 0.0 0.0 0.0 0.02464235811638009 2.3302466303905522E-4 35 0.0 0.0 0.0 0.03265258090834761 2.3302466303905522E-4 36 0.0 0.0 0.0 0.044507710640459545 2.3302466303905522E-4 37 0.0 0.0 0.0 0.05977082606951767 2.3302466303905522E-4 38 0.0 0.0 0.0 0.07590778398497224 2.3302466303905522E-4 39 0.0 0.0 0.0 0.0936759145417002 2.3302466303905522E-4 40 0.0 0.0 0.0 0.10987112862291454 2.3302466303905522E-4 41 0.0 0.0 0.0 0.1274644906823632 2.3302466303905522E-4 42 0.0 0.0 0.0 0.14622297605700715 2.3302466303905522E-4 43 0.0 0.0 0.0 0.16603007241532686 2.621527459189371E-4 44 0.0 0.0 0.0 0.19038114970290812 2.621527459189371E-4 45 0.0 0.0 0.0 0.21784893185863674 2.621527459189371E-4 46 0.0 0.0 0.0 0.24677311815835948 2.621527459189371E-4 47 0.0 0.0 0.0 0.2791344182379083 2.91280828798819E-4 48 0.0 0.0 0.0 0.3103305950022618 3.2040891167870096E-4 49 0.0 0.0 0.0 0.3414102594350958 3.2040891167870096E-4 50 0.0 0.0 0.0 0.37301422935976764 3.4953699455858285E-4 51 0.0 0.0 0.0 0.4043560465385206 3.4953699455858285E-4 52 0.0 0.0 0.0 0.43762031718734573 3.4953699455858285E-4 53 0.0 0.0 0.0 0.472574016643204 3.4953699455858285E-4 54 0.0 0.0 0.0 0.5126542586859215 3.4953699455858285E-4 55 0.0 0.0 0.0 0.555938589845426 3.4953699455858285E-4 56 0.0 0.0 0.0 0.6029513156135554 3.4953699455858285E-4 57 0.0 0.0 0.0 0.6525855688408742 3.4953699455858285E-4 58 0.0 0.0 0.0 0.7045500686985835 3.4953699455858285E-4 59 0.0 0.0 0.0 0.7591652240983621 3.4953699455858285E-4 60 0.0 0.0 0.0 0.8143920692386182 3.4953699455858285E-4 61 0.0 0.0 0.0 0.8721821856723039 3.4953699455858285E-4 62 0.0 0.0 0.0 0.9296518931943109 3.4953699455858285E-4 63 0.0 0.0 0.0 0.9895101035124682 3.4953699455858285E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3280 0.0 38.910934 1 GTATCAA 6490 0.0 33.681507 1 TATCAAC 9015 0.0 24.157196 2 ATCAACG 8970 0.0 24.012484 3 TCAACGC 9000 0.0 23.85596 4 CAACGCA 9065 0.0 23.690176 5 AACGCAG 9535 0.0 22.631395 6 CGTGCCG 660 0.0 21.971703 10 TATAGCG 110 3.250429E-5 21.97106 5 GTACATG 8305 0.0 20.906591 1 TACATGG 8610 0.0 19.753029 2 GCACCGT 900 0.0 19.564991 6 ACGCAGA 11180 0.0 19.268896 7 GTGCCGA 760 0.0 19.08069 11 CGCAGAG 11495 0.0 18.591238 8 GAGTACT 7510 0.0 18.435833 12 AGAGTAC 11050 0.0 18.43521 11 CCGATCG 170 2.4187138E-6 18.277647 9 ACATGGG 9070 0.0 18.144339 3 CGAATCG 535 0.0 18.050177 37 >>END_MODULE