FastQCFastQC Report
Mon 6 Feb 2017
SRR2077489_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077489_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2821349
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24550.037.5412141
GTATCAA46850.033.891971
TTCGCGA500.007755764827.58615116
ATCAACG59800.025.8986663
TCAACGC59650.025.791234
TATCAAC61400.025.448042
CAACGCA61750.024.859145
AACGCAG64100.023.9286426
ACGCAGA73900.020.6173327
CGCAGAG75600.020.0632348
GTACATG72050.019.4509431
TACATGG73650.018.8268572
AGAGTAC75050.018.78902811
CGAATCG950.00782056818.16458737
ACATGGG75700.017.6338583
CAGAGTA81550.017.5861310
GAGTACT46000.017.4631312
GCAGAGT87150.016.8912339
GTACTTT49100.016.71432714
AGTACTT51250.015.8777313
Produced by FastQC (version 0.11.5)