FastQCFastQC Report
Mon 6 Feb 2017
SRR2077489_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077489_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2821349
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30000.037.242261
GTATCAA58950.034.0915761
AACGCGT450.004633749430.6532242
ATCAACG79200.025.106683
TCAACGC79300.024.9878034
TATCAAC81650.024.6462972
CAACGCA81250.024.3088045
AACGCAG85400.023.0887286
ACGCAGA99350.019.6740367
TCGGTCG900.005753940419.15656554
CGCAGAG102450.018.977948
AGAGTAC100600.018.70935611
GTACATG72350.018.6936021
GAGTACT71100.018.45756112
TACATGG70500.018.2938292
GTGGTAT15700.018.286611
CAGAGTA110250.017.19705410
AGTACTT77150.017.17974313
ACATGGG73000.017.006633
GCAGAGT114200.016.7534399
Produced by FastQC (version 0.11.5)