FastQCFastQC Report
Mon 6 Feb 2017
SRR2077488_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077488_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3568434
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26650.038.2213361
GTATCAA54850.033.427121
TCAACGC69600.025.8772984
ATCAACG71250.025.4695873
CAACGCA72200.024.9461275
TATCAAC72900.024.8450932
AACGCAG74950.024.055916
GTACATG94300.020.2485771
ACGCAGA88500.019.9840817
GAGTACT47650.019.46390312
TACATGG94750.019.443342
CGCAGAG90650.019.282738
AGAGTAC88600.019.14599811
ACATGGG98600.018.4397373
CAGAGTA95850.017.69756510
GTACTTT52500.017.60260814
AGTACTT54750.017.0674413
GCAGAGT102100.016.7492929
CATGGGG73550.016.6065034
GTATACG1250.001930901216.5738071
Produced by FastQC (version 0.11.5)