FastQCFastQC Report
Mon 6 Feb 2017
SRR2077488_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077488_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3568434
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35200.037.2442321
GTATCAA66650.032.5928231
ATCAACG89950.024.0628173
TCAACGC90550.024.0179254
TATCAAC91800.023.6895522
CAACGCA92350.023.5164185
CGAATCG1803.45608E-1022.9921237
AACGCAG95950.022.6694416
GTACATG90550.019.6352831
TACATGG90600.019.1568622
ACGCAGA113200.019.0025227
CGCAGAG116550.018.3386048
CGTTATG2701.5279511E-1017.8837667
AGAGTAC114800.017.86721411
GAGTACT77600.017.84411212
ACATGGG94700.017.4887523
GTGGTAT19700.017.0320911
TGGTATC18300.017.0035782
GTACTTT81350.016.83475114
CGTAACG1452.7333156E-416.64806652
Produced by FastQC (version 0.11.5)