FastQCFastQC Report
Mon 6 Feb 2017
SRR2077487_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077487_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3551123
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29600.036.3773231
GTATCAA57800.033.974441
TATCAAC75750.025.5408542
ATCAACG75700.025.4228463
TCAACGC76650.025.2888094
CAACGCA77950.024.9576685
AACGCAG80800.024.1463746
GTACATG99850.021.1183991
ACGCAGA94100.020.4064567
TACATGG102700.020.0138662
CGCAGAG98050.019.446478
AGAGTAC94150.018.82454311
GAGTACT48000.018.10311112
ACATGGG109750.017.975353
CAGAGTA104350.017.3144310
GCAGAGT109800.016.8946559
GTATACG1653.833958E-516.7330041
TAGCGCG1653.853495E-516.72308329
AGTACTT53650.016.45372213
GTACTTT53500.016.17736214
Produced by FastQC (version 0.11.5)