FastQCFastQC Report
Mon 6 Feb 2017
SRR2077487_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077487_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3551123
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38350.040.0542951
GTATCAA72750.034.2397841
TATCAAC101500.024.3251292
ATCAACG102700.024.0449733
TCAACGC103650.023.8249284
CAACGCA105000.023.283485
AACGCAG109250.022.4383986
CGTGCCG4900.019.7306510
ATTACGC900.00565507919.2142753
ACGCAGA128250.019.0864627
GCACCGT7800.019.0339456
GTACATG92600.018.830031
GTGGTAT21050.018.7363191
GAGTACT84450.018.6442412
CGCAGAG131550.018.5827338
AGAGTAC127900.018.24969511
TACATGG93950.018.2192862
AGTACTT88700.017.97636413
GTACTTT88900.017.61814514
TCGCACG1804.186042E-617.25653313
Produced by FastQC (version 0.11.5)