FastQCFastQC Report
Mon 6 Feb 2017
SRR2077486_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077486_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3678493
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56900.032.6905941
GGTATCA30550.031.4036331
TCAACGC70450.025.6560294
ATCAACG70900.025.3465463
CAACGCA72300.024.9054875
TATCAAC73850.024.4718442
AACGCAG75600.023.8027736
GTACATG86500.021.3842981
ACGCAGA87200.020.4406327
TACATGG87450.019.9561422
CGCAGAG90500.019.5833978
CGTACCG900.005741058419.1641855
ACATGGG88200.018.7324373
AGAGTAC86900.018.65035211
GAGTACT56250.017.53332712
CAGAGTA97850.016.91544710
GCAGAGT102100.016.5150649
CATGGGG57600.016.4683234
AGTACTT60850.016.37834213
GTACTTT59900.016.06260514
Produced by FastQC (version 0.11.5)