##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077486_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3678493
Sequences flagged as poor quality	0
Sequence length	75
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.03091537757446	31.0	31.0	34.0	26.0	34.0
2	30.115448636167038	31.0	31.0	34.0	26.0	34.0
3	30.22452292283824	31.0	31.0	34.0	26.0	34.0
4	33.63164426301749	37.0	35.0	37.0	28.0	37.0
5	33.670344350254304	37.0	35.0	37.0	28.0	37.0
6	33.71079026112052	37.0	35.0	37.0	28.0	37.0
7	33.59624063441197	37.0	35.0	37.0	28.0	37.0
8	33.52889321795637	37.0	35.0	37.0	28.0	37.0
9	34.99103355640476	39.0	35.0	39.0	27.0	39.0
10	34.90759204924408	39.0	35.0	39.0	27.0	39.0
11	34.95256753241069	39.0	35.0	39.0	27.0	39.0
12	34.86759550718188	39.0	35.0	39.0	27.0	39.0
13	34.8410343583636	39.0	35.0	39.0	27.0	39.0
14	35.986858748949636	40.0	36.0	41.0	27.0	41.0
15	36.0107652780636	40.0	36.0	41.0	27.0	41.0
16	35.980957691097956	40.0	36.0	41.0	27.0	41.0
17	35.90858430340903	40.0	36.0	41.0	27.0	41.0
18	35.87269542173928	40.0	36.0	41.0	27.0	41.0
19	35.95491985440777	40.0	36.0	41.0	27.0	41.0
20	35.93190227628543	40.0	36.0	41.0	27.0	41.0
21	35.906643019301654	40.0	36.0	41.0	27.0	41.0
22	35.8355853878205	40.0	36.0	41.0	26.0	41.0
23	35.796325832344934	40.0	36.0	41.0	26.0	41.0
24	35.76824748613087	40.0	36.0	41.0	26.0	41.0
25	35.78734661177825	40.0	36.0	41.0	26.0	41.0
26	35.727696640988576	40.0	36.0	41.0	26.0	41.0
27	35.71150060636244	40.0	36.0	41.0	26.0	41.0
28	35.67266105984162	40.0	36.0	41.0	26.0	41.0
29	35.639772319805964	40.0	36.0	41.0	25.0	41.0
30	35.555696041830174	40.0	36.0	41.0	25.0	41.0
31	35.53948695838214	40.0	36.0	41.0	25.0	41.0
32	35.47442308575822	40.0	36.0	41.0	25.0	41.0
33	35.362943194400536	40.0	35.0	41.0	25.0	41.0
34	35.33221050033261	40.0	35.0	41.0	25.0	41.0
35	35.28431860547241	40.0	35.0	41.0	24.0	41.0
36	35.21755050233887	40.0	35.0	41.0	24.0	41.0
37	35.149269823267296	40.0	35.0	41.0	24.0	41.0
38	35.07439568323224	40.0	35.0	41.0	24.0	41.0
39	35.034772935547245	40.0	35.0	41.0	24.0	41.0
40	34.99837406242176	40.0	35.0	41.0	23.0	41.0
41	34.91583047731775	40.0	35.0	41.0	23.0	41.0
42	34.834394682822555	40.0	35.0	41.0	22.0	41.0
43	34.75303582200646	40.0	35.0	41.0	22.0	41.0
44	34.659921603765454	39.0	35.0	41.0	21.0	41.0
45	34.57378442748158	39.0	35.0	41.0	20.0	41.0
46	34.48940286144353	39.0	34.0	41.0	19.0	41.0
47	34.38842672801063	39.0	34.0	41.0	18.0	41.0
48	34.29516706977559	39.0	34.0	41.0	17.0	41.0
49	34.20610559813489	39.0	34.0	41.0	15.0	41.0
50	34.087654373679655	39.0	34.0	41.0	15.0	41.0
51	33.99167376422899	39.0	34.0	41.0	14.0	41.0
52	33.8931619551811	39.0	34.0	41.0	13.0	41.0
53	33.8155285330161	39.0	33.0	41.0	12.0	41.0
54	33.82293944830125	39.0	34.0	41.0	9.0	41.0
55	33.73292160675581	39.0	33.0	41.0	9.0	41.0
56	33.60150583404671	38.0	33.0	41.0	8.0	41.0
57	33.51347168527982	38.0	33.0	40.0	8.0	41.0
58	33.42714584477937	38.0	33.0	40.0	7.0	41.0
59	33.31403648178752	38.0	33.0	40.0	5.0	41.0
60	33.2943039445773	38.0	33.0	40.0	2.0	41.0
61	33.19125114550986	38.0	33.0	40.0	2.0	41.0
62	33.0445774940988	38.0	33.0	40.0	2.0	41.0
63	32.86021721395147	37.0	33.0	40.0	2.0	41.0
64	32.72194673199052	37.0	33.0	40.0	2.0	41.0
65	32.574663591856776	37.0	32.0	40.0	2.0	41.0
66	32.40938422337626	37.0	32.0	40.0	2.0	41.0
67	32.24447593076839	36.0	32.0	40.0	2.0	41.0
68	32.05576658702355	36.0	32.0	39.0	2.0	41.0
69	32.08283854284893	36.0	32.0	39.0	2.0	41.0
70	31.9808049111416	36.0	32.0	39.0	2.0	41.0
71	31.811998826693433	36.0	32.0	39.0	2.0	41.0
72	31.605136396888618	35.0	32.0	39.0	2.0	41.0
73	31.394239978164972	35.0	32.0	39.0	2.0	41.0
74	31.17060519076698	35.0	32.0	38.0	2.0	40.0
75	30.949204742268098	35.0	32.0	37.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6381757568771071
1101	2	0.5919853073533332
1101	3	0.5962074989760282
1101	4	0.691213844811287
1101	5	0.6577541715165793
1101	6	0.5717416900140861
1101	7	0.6477889544387097
1101	8	0.6449105663212436
1101	9	0.764436966024185
1101	10	0.852245813324906
1101	11	0.6994197097807131
1101	12	0.6875610644535826
1101	13	0.7290812519735894
1101	14	0.8538702493200034
1101	15	0.8941444814632717
1101	16	0.879162498876255
1101	17	0.9009622729045219
1101	18	0.9426033622615222
1101	19	0.8816570331921056
1101	20	0.8780915160592073
1101	21	0.8517181613287903
1101	22	0.8821643356450153
1101	23	0.9585266215092503
1101	24	0.8954091307568746
1101	25	0.8368894302830654
1101	26	0.7974080215928723
1101	27	0.88391168228776
1101	28	0.853463787624591
1101	29	0.9083752289664417
1101	30	0.9222117897577888
1101	31	0.916677130169127
1101	32	0.850843634874586
1101	33	0.9865303735342863
1101	34	0.8740443493336869
1101	35	0.8522861393276173
1101	36	1.0179059541865314
1101	37	1.0121985494966026
1101	38	0.913266577503606
1101	39	0.9427827259277777
1101	40	0.8832039526871185
1101	41	0.96390591370767
1101	42	0.7981871178996087
1101	43	0.7863713555896439
1101	44	0.8459265160174851
1101	45	0.902096251813937
1101	46	0.8843618664733839
1101	47	0.8338435729409213
1101	48	0.7496412437077637
1101	49	0.8131582234459671
1101	50	0.6952309464037256
1101	51	0.7882968973988369
1101	52	0.7459383227203986
1101	53	0.7129757333575668
1101	54	0.7392635828428737
1101	55	0.6522509011359219
1101	56	0.6050567476537623
1101	57	0.6579972846106017
1101	58	0.709632443610289
1101	59	0.7083001342602628
1101	60	0.7357397926100333
1101	61	0.6355817444316827
1101	62	0.6423914420669306
1101	63	0.6906016029119044
1101	64	0.6969540981995266
1101	65	0.66594629624975
1101	66	0.5740434681847688
1101	67	0.5555727750355999
1101	68	0.5901409008427407
1101	69	0.582042934816748
1101	70	0.6308787960984148
1101	71	0.598960529898303
1101	72	0.6356739544086984
1101	73	0.6154014053833308
1101	74	0.594915893883357
1101	75	0.6317538829503917
1102	1	0.7772264819657089
1102	2	0.7911593426457628
1102	3	0.7976049091092463
1102	4	0.8331725857825276
1102	5	0.7810295929607278
1102	6	0.7898177745895936
1102	7	0.856872731783973
1102	8	0.9293096062267452
1102	9	0.972705893198075
1102	10	1.0182339657328896
1102	11	0.9848095620293194
1102	12	0.9121356636738369
1102	13	1.0296492443394527
1102	14	1.110836455734571
1102	15	1.0700809710611097
1102	16	1.131306399387853
1102	17	1.200679014093737
1102	18	1.1423429799718363
1102	19	1.1096841166910423
1102	20	1.0671758904960598
1102	21	1.1431514077481353
1102	22	1.1642917298114313
1102	23	1.2100651215072702
1102	24	1.1472364648184694
1102	25	1.1831508995672024
1102	26	1.1181906216245991
1102	27	1.12991636621949
1102	28	1.198830231005381
1102	29	1.179190615081474
1102	30	1.2501857105237306
1102	31	1.2490392424777355
1102	32	1.2077859216562388
1102	33	1.2696692624331618
1102	34	1.2024333206923572
1102	35	1.2278515688756642
1102	36	1.1939146343155755
1102	37	1.1676023542649219
1102	38	1.1985460757164645
1102	39	1.2287488794673607
1102	40	1.247435073172646
1102	41	1.2600355732251813
1102	42	1.1848671915504951
1102	43	1.3046338694243644
1102	44	1.268100807052157
1102	45	1.3287269682914697
1102	46	1.3717961459331107
1102	47	1.404653311864422
1102	48	1.3299581914331142
1102	49	1.2801968613865284
1102	50	1.2816579755169784
1102	51	1.2911649872776678
1102	52	1.3122069642974665
1102	53	1.2922394259086332
1102	54	1.3462618199839156
1102	55	1.3303936081837193
1102	56	1.258129665125999
1102	57	1.2392941819284715
1102	58	1.1373966629328223
1102	59	1.0829008652298526
1102	60	1.190000454741444
1102	61	1.2850593072764696
1102	62	1.257654541842733
1102	63	1.175532306116743
1102	64	1.2270669736883022
1102	65	1.2458955650463537
1102	66	1.279488763624606
1102	67	1.1980009522912312
1102	68	1.2303421782590505
1102	69	1.2387119982426213
1102	70	1.209237319383007
1102	71	1.1386695304784844
1102	72	1.1995473235123733
1102	73	1.2189658263501641
1102	74	1.2564687348703139
1102	75	1.2993867708989626
1103	1	0.6449206356619115
1103	2	0.70799249536147
1103	3	0.7553379237900231
1103	4	0.7547121861544923
1103	5	0.6901125919720315
1103	6	0.7073779089814138
1103	7	0.688917613811789
1103	8	0.7172862137663429
1103	9	0.8413591828824352
1103	10	0.8956642809918165
1103	11	0.8356256563756261
1103	12	0.8363977543749854
1103	13	0.7722318747677619
1103	14	0.9000113937316314
1103	15	0.8648498949867403
1103	16	0.8652042781474591
1103	17	0.8998808448080311
1103	18	0.8546046099869642
1103	19	0.9331341286695647
1103	20	0.9214216692211892
1103	21	0.9171373431543444
1103	22	0.9415380782424876
1103	23	0.9889160106347035
1103	24	0.9149121295579761
1103	25	0.8655358109381126
1103	26	0.9414158612035379
1103	27	0.9200887851389226
1103	28	0.9179668479910958
1103	29	0.8473907373614864
1103	30	0.8428523614696601
1103	31	0.8068408474285391
1103	32	0.8683419368725467
1103	33	0.9461117684420728
1103	34	0.9433296967543185
1103	35	0.9172578751477971
1103	36	0.9603635345644079
1103	37	0.9790416187256952
1103	38	0.9936484160219479
1103	39	0.9496385639623739
1103	40	0.9774489040058683
1103	41	0.9816830998914838
1103	42	1.0723043726715318
1103	43	1.042825355550299
1103	44	1.0483151969285487
1103	45	0.9998454066342148
1103	46	1.0654542970852816
1103	47	1.0512588111841552
1103	48	1.043163134170321
1103	49	1.0362402383244103
1103	50	1.1473553560658445
1103	51	1.1054836077075834
1103	52	1.0553091993423465
1103	53	1.062185438838597
1103	54	1.0524065786039216
1103	55	1.1434420953872078
1103	56	1.072746022692165
1103	57	1.0982365999354187
1103	58	1.0171019400775023
1103	59	1.0078590429364098
1103	60	1.0557580867332845
1103	61	1.102466847968408
1103	62	1.047447475780423
1103	63	1.0441954725265816
1103	64	1.051880964873071
1103	65	1.0912073158548594
1103	66	1.0373102245529324
1103	67	1.0507280734034694
1103	68	1.0911026656156437
1103	69	1.1016335404745092
1103	70	1.1191483229095809
1103	71	1.1145409635366725
1103	72	1.1175232714644245
1103	73	1.0939186897632496
1103	74	1.2057322694872106
1103	75	1.2277132537491724
1104	1	0.7130441627726611
1104	2	0.7067477210493536
1104	3	0.7943276478300589
1104	4	0.7147135154127611
1104	5	0.6932553288486645
1104	6	0.7569369225924234
1104	7	0.7672345347734932
1104	8	0.8229528219059645
1104	9	0.8355123643562763
1104	10	0.9194181261689423
1104	11	0.7957702942238427
1104	12	0.8917945925258906
1104	13	0.9222141017250536
1104	14	0.9561081756370484
1104	15	0.9700060944059032
1104	16	0.9374486381410136
1104	17	0.982931313966759
1104	18	0.9961266040859869
1104	19	0.9847012811768181
1104	20	1.0186640535137172
1104	21	0.9966168691051394
1104	22	1.0084965204047052
1104	23	1.0624749144661791
1104	24	0.9876100245353356
1104	25	0.9742065427543096
1104	26	1.0538015722723912
1104	27	1.021970280772706
1104	28	0.9944083905523229
1104	29	0.9353641574867879
1104	30	0.9787896852892075
1104	31	0.9804765969367679
1104	32	0.9715148631221879
1104	33	1.0816207867319392
1104	34	1.0780876510589152
1104	35	1.0810343309418542
1104	36	1.0895838055545326
1104	37	1.0282747987314025
1104	38	1.06803446899454
1104	39	1.0775909804844588
1104	40	1.068602392664019
1104	41	1.031407311867838
1104	42	1.0072313610120815
1104	43	1.0162605920706511
1104	44	0.9966183921413645
1104	45	0.9876369961328137
1104	46	0.9091464871639232
1104	47	0.9122237534854207
1104	48	0.9884369431863291
1104	49	1.0224566237140778
1104	50	1.000713369955598
1104	51	1.0025982575554977
1104	52	1.124870110948983
1104	53	1.0928238167731763
1104	54	1.0068861954865582
1104	55	1.069346528331259
1104	56	1.134593268432667
1104	57	1.0327510883545017
1104	58	1.0154065406006723
1104	59	1.031961041353867
1104	60	1.0864120057470643
1104	61	1.0497492918725513
1104	62	0.9309375260177859
1104	63	0.982780467184746
1104	64	0.9765819561624625
1104	65	0.96689018922185
1104	66	1.0047974932677803
1104	67	0.9953956480659514
1104	68	0.998514305487177
1104	69	1.02410289892223
1104	70	0.9987426544497353
1104	71	0.9442226723147051
1104	72	0.9848612466205218
1104	73	1.0167403677831715
1104	74	0.9842607475228711
1104	75	0.9741533380942471
1105	1	0.5208617507743156
1105	2	0.5346760126854981
1105	3	0.5208800007859296
1105	4	0.5121550349970221
1105	5	0.5282414808215989
1105	6	0.5481589575956534
1105	7	0.49909289407439417
1105	8	0.5522446425850944
1105	9	0.6100708351021709
1105	10	0.5963346697465113
1105	11	0.5599051531367465
1105	12	0.5913413184364842
1105	13	0.6183520453192202
1105	14	0.6698745730790563
1105	15	0.6355357482011996
1105	16	0.75303562607224
1105	17	0.6870015944165004
1105	18	0.6750040315198262
1105	19	0.6489881984375785
1105	20	0.6811570529472277
1105	21	0.7580130661153817
1105	22	0.7912667865914287
1105	23	0.7248609009235523
1105	24	0.7787829044817656
1105	25	0.7669090313700764
1105	26	0.8307032081687993
1105	27	0.6851253424139543
1105	28	0.7575340397415431
1105	29	0.802133646862444
1105	30	0.793642248322513
1105	31	0.7228240007036533
1105	32	0.7702838915684609
1105	33	0.7712206242785058
1105	34	0.7841389608800142
1105	35	0.7562267407564249
1105	36	0.7545954778659762
1105	37	0.6713841058787864
1105	38	0.7046646561624925
1105	39	0.7999640254629483
1105	40	0.7825415580433912
1105	41	0.7877179750501355
1105	42	0.6327890895972033
1105	43	0.64411668218073
1105	44	0.6906059228733454
1105	45	0.7410702351045515
1105	46	0.7344808422777831
1105	47	0.7254952964948842
1105	48	0.6019677992659638
1105	49	0.6095196574191348
1105	50	0.6495830051042049
1105	51	0.6285460033126498
1105	52	0.6090195390983908
1105	53	0.5710551372472281
1105	54	0.554222595773112
1105	55	0.6255714522013562
1105	56	0.6277234060426551
1105	57	0.6175041567179065
1105	58	0.6962983583788471
1105	59	0.5740376893980184
1105	60	0.5564968022491641
1105	61	0.617377221936998
1105	62	0.7165776417013916
1105	63	0.7368409569141576
1105	64	0.7256906782840886
1105	65	0.8135953902297715
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2212	73	-0.5679418111556451
2212	74	-0.4936591954124374
2212	75	-0.5401961416607293
2213	1	-0.39832311502865636
2213	2	-0.3900840645445349
2213	3	-0.4455142468999611
2213	4	-0.4174807247052499
2213	5	-0.4851916481864009
2213	6	-0.38802069001381767
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2213	9	-0.49486999522283526
2213	10	-0.5603789711717297
2213	11	-0.5983771580740012
2213	12	-0.6188820538774991
2213	13	-0.6551694899615015
2213	14	-0.8212404647654665
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2213	16	-0.6405307385103001
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2213	18	-0.6917148748133712
2213	19	-0.6681489138856946
2213	20	-0.6703305489027329
2213	21	-0.6920305804294671
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2213	24	-0.6145790311422132
2213	25	-0.6469236592924972
2213	26	-0.5451309796657355
2213	27	-0.6549041125275252
2213	28	-0.721561463517105
2213	29	-0.5775096401479658
2213	30	-0.6290246451255967
2213	31	-0.5491063341705171
2213	32	-0.5381573072763715
2213	33	-0.5231396677721705
2213	34	-0.5633975813514382
2213	35	-0.6258692496678293
2213	36	-0.7573062695205266
2213	37	-0.6808763884248137
2213	38	-0.643731701538151
2213	39	-0.6048500470653693
2213	40	-0.6190010590291948
2213	41	-0.5641447339547909
2213	42	-0.584593115431872
2213	43	-0.46541383731603503
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2213	47	-0.4360647762350425
2213	48	-0.5212833524417917
2213	49	-0.5499339828744212
2213	50	-0.5208258069237388
2213	51	-0.5734779469979401
2213	52	-0.43537008502914176
2213	53	-0.4019965074881071
2213	54	-0.3870816609825596
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2213	57	-0.35605407511518905
2213	58	-0.3980500024813409
2213	59	-0.5321599719406152
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2213	70	-0.43308837610679873
2213	71	-0.4403447525209927
2213	72	-0.4015053030290474
2213	73	-0.43939704035208393
2213	74	-0.4683175743464929
2213	75	-0.4594612833721463
2214	1	-0.6719440619384898
2214	2	-0.6922528308700393
2214	3	-0.6277808801817599
2214	4	-0.573641475222189
2214	5	-0.5959610651886464
2214	6	-0.5681325067350258
2214	7	-0.6256725446768243
2214	8	-0.6030038996035216
2214	9	-0.7519571493366115
2214	10	-0.6893611811326039
2214	11	-0.6493715545346816
2214	12	-0.7108120632675892
2214	13	-0.824030534132767
2214	14	-0.890917171884162
2214	15	-0.8089431726621399
2214	16	-0.868982526590429
2214	17	-0.8456768392556313
2214	18	-1.1227936277864288
2214	19	-0.9640467434891207
2214	20	-0.9134372120975272
2214	21	-0.794402879409212
2214	22	-0.8026613563465474
2214	23	-0.8045792146933337
2214	24	-0.7718941902069787
2214	25	-0.9110860515996322
2214	26	-0.9477706848675851
2214	27	-1.010444690001215
2214	28	-0.8754646334112905
2214	29	-0.936140046882727
2214	30	-0.9299322690730634
2214	31	-0.9488933375892614
2214	32	-0.9919602034762249
2214	33	-0.8996154522003152
2214	34	-0.9480540520482137
2214	35	-0.9260182509416737
2214	36	-0.9535138812966863
2214	37	-0.995459492779716
2214	38	-0.9563420428950664
2214	39	-0.9420367195589492
2214	40	-0.9275580290248655
2214	41	-0.9529846785442402
2214	42	-0.9502162156497533
2214	43	-0.9053807421537883
2214	44	-0.8592365131340856
2214	45	-0.9002246238017477
2214	46	-0.9703536187971054
2214	47	-0.8895670815984431
2214	48	-0.8354918959489765
2214	49	-0.9398983245652701
2214	50	-0.920545985913698
2214	51	-0.8787411475154485
2214	52	-0.8615089909711244
2214	53	-0.8943408230435921
2214	54	-0.8197063210281641
2214	55	-0.8565074016128733
2214	56	-0.7374787314762798
2214	57	-0.7991354747061408
2214	58	-0.762490694054101
2214	59	-0.7802707659437189
2214	60	-0.7677792464962323
2214	61	-0.9858695486472371
2214	62	-0.8748119440690019
2214	63	-0.9451713903459975
2214	64	-0.9633253888027191
2214	65	-0.9685457010402665
2214	66	-0.8686781481566292
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2214	68	-0.8762954508802494
2214	69	-0.8609477498747964
2214	70	-0.907947239605079
2214	71	-0.9185279262462736
2214	72	-0.9231597657906754
2214	73	-0.9380437331925755
2214	74	-0.9192920372785593
2214	75	-0.9789776555292207
2215	1	-0.9899559675056757
2215	2	-1.0835393928146466
2215	3	-1.2463028759312635
2215	4	-1.4056488718420113
2215	5	-1.1338795580672496
2215	6	-1.2111840704531147
2215	7	-1.1200592624715924
2215	8	-1.1943937899726436
2215	9	-1.3068933808745626
2215	10	-1.2252275876664314
2215	11	-1.1789335126378617
2215	12	-1.2008028928828622
2215	13	-1.4200829758730222
2215	14	-1.602752527868894
2215	15	-1.540767076093644
2215	16	-1.584931381870085
2215	17	-1.8510691974963507
2215	18	-1.62901837260209
2215	19	-1.5632920449461238
2215	20	-1.4980174370049895
2215	21	-1.7474295351323121
2215	22	-1.5600685009874056
2215	23	-1.7022701881430038
2215	24	-1.6030213107240527
2215	25	-1.6518547395325314
2215	26	-1.6046981388781631
2215	27	-1.6376330969892976
2215	28	-1.6870771275701841
2215	29	-1.6005403916251524
2215	30	-1.6060189539630585
2215	31	-1.518690659832771
2215	32	-1.7047617439619955
2215	33	-1.5160977193571483
2215	34	-1.587571169128175
2215	35	-1.5002779652423612
2215	36	-1.5918091871669873
2215	37	-1.7492239244385388
2215	38	-1.5679429979705404
2215	39	-1.67440120340013
2215	40	-1.7371360537602172
2215	41	-1.794682599485867
2215	42	-1.7676763877416803
2215	43	-1.6727042185951575
2215	44	-1.6760163870865625
2215	45	-1.65593854914637
2215	46	-1.7880877081070707
2215	47	-1.7741321274556796
2215	48	-1.8003011890200113
2215	49	-1.7535959367962661
2215	50	-1.8467368373273274
2215	51	-1.7723506948297398
2215	52	-1.6863911719812066
2215	53	-1.681426106792479
2215	54	-1.785959813032271
2215	55	-1.7561673509906441
2215	56	-1.5639335335938256
2215	57	-1.552787080347315
2215	58	-1.5915504688637334
2215	59	-1.608838303248131
2215	60	-1.7044315179793088
2215	61	-1.7438479737737502
2215	62	-1.8170296859512085
2215	63	-1.6361902813074387
2215	64	-1.6437268722200251
2215	65	-1.7028477612381678
2215	66	-1.6998067712934564
2215	67	-1.6300407953324942
2215	68	-1.6133486264948118
2215	69	-1.670970342799066
2215	70	-1.6790972926903045
2215	71	-1.7509658752397428
2215	72	-1.792197542247127
2215	73	-1.708020836117342
2215	74	-1.6887120827908362
2215	75	-1.7409032554883694
2216	1	-1.0853531394242317
2216	2	-0.9570489484838305
2216	3	-1.217028777424236
2216	4	-1.0634440867810468
2216	5	-1.0312990089082703
2216	6	-1.155190330476401
2216	7	-1.0949625108142769
2216	8	-1.181127144212006
2216	9	-1.3118256966281905
2216	10	-1.3396955791620684
2216	11	-1.29205901271839
2216	12	-1.240008945629313
2216	13	-1.4176570369405823
2216	14	-1.6504121725034295
2216	15	-1.6044197285506243
2216	16	-1.567973458343026
2216	17	-1.5951161223271768
2216	18	-1.791423982259623
2216	19	-1.6287031452652556
2216	20	-1.5714212474872724
2216	21	-1.7423125381579538
2216	22	-1.5815528222609743
2216	23	-1.6919761982195638
2216	24	-1.6829843942960494
2216	25	-1.6507142945355326
2216	26	-1.5348545588920146
2216	27	-1.6024013799412629
2216	28	-1.6162180224978826
2216	29	-1.748605138154673
2216	30	-1.853107852014304
2216	31	-1.7193261686942023
2216	32	-1.67239583203218
2216	33	-1.5684526161198775
2216	34	-1.6864575463211722
2216	35	-1.686951412158166
2216	36	-1.6968996591934626
2216	37	-1.7868257301896122
2216	38	-1.8777010681787658
2216	39	-1.8426842283747504
2216	40	-1.7724909959175505
2216	41	-1.806984068803196
2216	42	-1.6145182410900887
2216	43	-1.8178987535224493
2216	44	-1.8396388903277412
2216	45	-1.982083707647206
2216	46	-1.8740964356049616
2216	47	-1.7412916450192029
2216	48	-1.9078702952700652
2216	49	-1.87863258036122
2216	50	-1.8301441304964214
2216	51	-1.9501345686783083
2216	52	-1.8858067887639436
2216	53	-1.8771113610777626
2216	54	-1.8622117766228214
2216	55	-2.050702114016847
2216	56	-1.9614590138269214
2216	57	-1.8295310682930541
2216	58	-1.8341722611487015
2216	59	-1.7623296739574705
2216	60	-2.002490164526961
2216	61	-2.003127527195108
2216	62	-1.921950573527642
2216	63	-1.9580501210631702
2216	64	-1.978666590606064
2216	65	-2.1357220823430403
2216	66	-2.127455263090866
2216	67	-2.003377650724179
2216	68	-2.021663917361998
2216	69	-1.8946949153329626
2216	70	-1.8344055949520595
2216	71	-1.803560808049074
2216	72	-1.8671362171568937
2216	73	-1.9047201032043226
2216	74	-2.0175123173930913
2216	75	-1.948432773781704
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	135926.0
3	21251.0
4	14970.0
5	11049.0
6	8884.0
7	8087.0
8	7720.0
9	8086.0
10	8625.0
11	8944.0
12	9716.0
13	10446.0
14	10675.0
15	11028.0
16	11825.0
17	12462.0
18	12979.0
19	14005.0
20	15493.0
21	17689.0
22	21672.0
23	25612.0
24	27876.0
25	29957.0
26	32487.0
27	35850.0
28	41329.0
29	49806.0
30	57022.0
31	64124.0
32	78248.0
33	98189.0
34	127426.0
35	169274.0
36	236714.0
37	368023.0
38	621172.0
39	1011427.0
40	222425.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.62085639139703	17.976125014264476	13.10054800094452	24.302470593393977
2	15.535341569664576	19.663705521579267	39.765540023365425	25.035412885390727
3	19.16948821753565	24.24229144318142	29.108850846002525	27.4793694932804
4	12.500629595879762	16.060208151884936	38.15718579217864	33.28197646005667
5	13.799206794115781	37.46295545561148	34.867625225874725	13.87021252439801
6	32.404717705276795	36.94024203689892	17.36799843214964	13.287041825674638
7	28.4783499834234	31.896232131162535	21.329807880226053	18.295610005188014
8	26.112529505537	35.271369301832465	19.880078394789933	18.736022797840608
9	26.034164780478143	16.020300070003994	19.453408149423147	38.49212700009472
10	13.865399716651039	29.422128710108968	34.41650119831297	22.29597037492702
11	34.946371216052206	23.342337255602313	23.43759443364857	18.273697094696907
12	22.967417700290756	25.031907131733984	30.656927550120333	21.343747617854927
13	27.77684141394146	22.18445302631733	25.991430047222693	24.047275512518517
14	21.440670881915754	21.59279996754289	27.70388551353709	29.262643637004267
15	23.36725909703963	29.527366123277282	23.835743910214703	23.26963086946839
16	22.992081994630578	28.788972347285792	25.717059608425867	22.50188604965776
17	21.98956770376242	28.37517067763649	26.924546874013227	22.71071474458786
18	21.810349520905923	28.125898301393725	27.822408536585364	22.241343641114984
19	22.75934523512895	27.66730415587959	27.95233617602872	21.621014432962742
20	22.82191578679696	27.250164511880932	28.02187973984117	21.906039961480943
21	23.176204767607324	26.97551608780205	27.81131502118156	22.03696412340907
22	22.673749005526577	26.978260171128387	27.715572705060282	22.632418118284754
23	22.609802690790556	27.00125675522956	27.491976782025812	22.89696377195407
24	22.29392302981837	27.024896607889247	27.868588212006035	22.812592150286346
25	22.762026023190735	26.920235039388846	27.711198414913973	22.606540522506453
26	22.461617472821597	27.09744740979041	27.839596286695915	22.60133883069208
27	22.631825205880286	27.328248806986448	27.471475796300123	22.568450190833143
28	22.50594725023932	27.144244137515948	27.72160741203721	22.628201200207524
29	22.570159737935228	27.28574058681331	27.72201590298287	22.42208377226859
30	22.77876965107362	27.249334434258227	27.5990287750976	22.372867139570552
31	22.76084963046342	26.94140495527895	27.651881481392714	22.645863932864913
32	22.534602217461064	27.24727882301811	27.577616038741848	22.640502920778978
33	22.62514975539385	27.10527010984468	27.412093345508946	22.857486789252526
34	22.745266385254258	27.102264818754847	27.70298778914934	22.449481006841552
35	22.87794919170604	27.128805202190602	27.514649953536306	22.47859565256705
36	22.695115431136923	27.15430919034106	27.577859468245713	22.572715910276305
37	22.827335007563455	26.84559626537174	27.58156559857541	22.745503128489396
38	22.559278497942106	27.08441040196338	27.70518327644958	22.651127823644938
39	22.698938267913547	27.390204310377932	27.425659827999894	22.485197593708634
40	22.928121338874526	26.970589509129645	27.726315267626994	22.37497388436884
41	22.78549346988247	27.029838347246997	27.538220594043228	22.646447588827304
42	22.685017515604624	27.177896138119163	27.45332237841609	22.683763967860127
43	22.62271833398848	27.01191272987597	27.58015847136992	22.785210464765633
44	22.711686217687703	27.290710564088037	27.4249031073731	22.57270011085116
45	22.871588414902348	27.153127542237048	27.310819434068584	22.664464608792017
46	22.7696958370271	27.10770833526308	27.1974212376294	22.92517459008042
47	22.74304609786126	27.16949217175294	27.24025691032108	22.847204820064718
48	22.52382890813984	27.308928695013407	27.383749096079097	22.783493300767656
49	22.772629311225735	27.210244456864103	27.19463056524195	22.82249566666821
50	22.831634460187736	27.192914102372683	27.29870996077679	22.67674147666279
51	22.677948976188453	27.457670902301366	26.996612364216627	22.867767757293553
52	22.752835927598163	27.42245812722523	26.98411401033783	22.84059193483878
53	22.7377055030843	27.34662475988827	27.136418877447195	22.779250859580237
54	22.754635524837425	27.35639916924858	27.20234244663103	22.686622859282966
55	22.747650756995704	27.151253960121362	27.125295774862725	22.97579950802021
56	22.68726469569785	27.58129449480223	27.077304997966223	22.654135811533703
57	22.87835040313794	27.192362170407495	26.9916376116801	22.93764981477446
58	22.656900505255265	27.179240286151472	27.24190676100423	22.92195244758903
59	22.8437712034138	27.38929263367858	27.02289764320224	22.744038519705377
60	22.66088460714677	27.41993851338868	27.238890363409745	22.680286516054807
61	23.024038597768442	27.122490371264846	27.128974918425484	22.724496112541225
62	22.751370619488124	27.414215529318408	27.138333990518547	22.696079860674917
63	22.66834700470387	27.14132037176389	27.208584768538795	22.981747854993444
64	22.682433343824467	27.19269490008216	27.264007368319227	22.86086438777415
65	22.70941994540047	27.174959442840574	27.092509572361823	23.023111039397136
66	22.591778333177576	27.32243682887362	27.139886911688617	22.945897926260187
67	22.738371649389013	27.209304860634205	27.04268724723939	23.00963624273739
68	22.730509941194164	27.160836577317248	27.18816341832353	22.92049006316505
69	22.69231345530133	27.35009065188993	27.014508388875498	22.943087503933242
70	22.71819366517514	26.983123644688977	27.181991143462238	23.11669154667365
71	22.619076160575048	27.111124672661113	27.19356557729884	23.076233589465
72	22.62465209440801	27.32372056833117	26.990989871778943	23.060637465481882
73	22.739051596002042	27.038564055845903	27.169412031366463	23.052972316785592
74	22.634281515875248	27.20181585533526	27.054244918718844	23.109657710070646
75	22.661092125711114	27.21422734175546	27.03402575468441	23.09065477784902
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2395.0
1	1981.0
2	1859.0
3	1967.0
4	1783.0
5	1528.0
6	1155.5
7	1088.0
8	1138.0
9	1464.0
10	2086.0
11	2451.0
12	2520.0
13	2563.0
14	2766.0
15	3161.0
16	3396.0
17	3753.0
18	4527.5
19	5775.5
20	6606.0
21	7903.5
22	11024.0
23	15114.0
24	17381.0
25	20821.0
26	28687.0
27	38027.0
28	42941.0
29	49388.0
30	62001.0
31	74119.0
32	80071.0
33	86599.5
34	99499.5
35	112285.5
36	118700.0
37	124082.5
38	134269.5
39	144018.0
40	148962.0
41	154477.0
42	162678.5
43	170030.5
44	174696.0
45	177903.0
46	182125.0
47	183780.0
48	184420.0
49	183930.5
50	180320.0
51	173692.0
52	170185.0
53	165078.0
54	154607.5
55	141134.0
56	133024.0
57	125898.5
58	110588.0
59	92562.5
60	82722.0
61	73986.5
62	56988.5
63	42213.0
64	35700.0
65	30188.0
66	20976.0
67	14789.0
68	12302.0
69	10358.5
70	7071.0
71	4911.5
72	4096.0
73	3628.5
74	2800.5
75	1840.0
76	1240.0
77	1038.5
78	648.0
79	386.5
80	314.0
81	244.0
82	130.5
83	75.0
84	63.0
85	52.5
86	40.5
87	33.0
88	27.0
89	27.0
90	20.5
91	15.0
92	16.0
93	12.5
94	9.5
95	12.0
96	14.0
97	11.5
98	7.0
99	7.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18442334945315922
2	0.12747067888942565
3	0.1273891237525802
4	0.1495721209745404
5	0.15087700316406746
6	0.12703571815958328
7	0.12611142660866828
8	0.10218858646733867
9	0.12007634648210559
10	0.12445313882614428
11	0.14350985580236256
12	0.1449778482655805
13	0.16022865885567814
14	0.16185976159258697
15	0.1461196201814167
16	0.14875656960608596
17	0.13323390855983688
18	0.1330164281949157
19	0.1705589761894341
20	0.14968086115700097
21	0.16199568682066268
22	0.15481883477826383
23	0.17229881910880354
24	0.1583257056626178
25	0.18398838872331685
26	0.16811232208407084
27	0.1821126205758717
28	0.18018248233719625
29	0.18292817194432612
30	0.17477265825978192
31	0.18300972708117155
32	0.168139507129686
33	0.18137862434426272
34	0.19709158070981783
35	0.1851029755935379
36	0.18784866520066779
37	0.2053014644855923
38	0.19744498630281476
39	0.17088519673681585
40	0.19899453390287816
41	0.19146427626748236
42	0.24216438633973206
43	0.20513835421190146
44	0.18765836988136175
45	0.18420586908823802
46	0.21462593513158784
47	0.19499833219745152
48	0.22756601684439798
49	0.23607493612193908
50	0.22337951981966528
51	0.23588464080263305
52	0.2198998339809264
53	0.21220646607183974
54	0.19350315468861842
55	0.1958410686115211
56	0.21693666400887535
57	0.1982605376712692
58	0.22479314219165294
59	0.23604775107632392
60	0.23789633417815392
61	0.22370574036704705
62	0.23928277150452645
63	0.25526757832623304
64	0.23727107812900552
65	0.26089488276856854
66	0.2246572169635772
67	0.24501881612932253
68	0.22044353489322938
69	0.2136200884438274
70	0.22318922450035925
71	0.21707258923695114
72	0.216311407959727
73	0.21261424175606694
74	0.2097598119664765
75	0.23689048749039346
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	3678493.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.723075635441326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.17445888982643	49.177938809606665
2	12.805590837769905	16.320232670234525
3	4.613833748468179	8.820230307689684
4	2.0671525535959643	5.269012740911653
5	1.0759591869833178	3.4281714326392954
6	0.6232841794768823	2.383055094670761
7	0.37259414183131534	1.661999127686748
8	0.26873856553240366	1.3699878350065091
9	0.1868287592865518	1.071477284300334
>10	0.7713224388332827	8.223664815080415
>50	0.03149667681742636	1.3503250072564639
>100	0.008611794400383756	0.8545600656446382
>500	8.548478542180882E-5	0.03720542404040636
>1k	4.274239256987086E-5	0.03213938523192135
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.718504561514729E-5	0.0
5	0.0	0.0	0.0	2.718504561514729E-5	0.0
6	0.0	0.0	0.0	2.718504561514729E-5	2.718504561514729E-5
7	0.0	0.0	0.0	2.718504561514729E-5	2.718504561514729E-5
8	0.0	0.0	0.0	5.437009123029458E-5	2.718504561514729E-5
9	0.0	0.0	0.0	8.155513684544187E-5	2.718504561514729E-5
10	0.0	0.0	0.0	1.0874018246058916E-4	2.718504561514729E-5
11	0.0	0.0	0.0	1.3592522807573645E-4	2.718504561514729E-5
12	0.0	0.0	0.0	1.9029531930603102E-4	8.155513684544187E-5
13	0.0	0.0	0.0	1.9029531930603102E-4	8.155513684544187E-5
14	0.0	0.0	0.0	2.718504561514729E-4	8.155513684544187E-5
15	2.718504561514729E-5	0.0	0.0	2.718504561514729E-4	8.155513684544187E-5
16	2.718504561514729E-5	0.0	0.0	2.990355017666202E-4	8.155513684544187E-5
17	2.718504561514729E-5	0.0	0.0	2.990355017666202E-4	8.155513684544187E-5
18	2.718504561514729E-5	2.718504561514729E-5	0.0	3.2622054738176747E-4	8.155513684544187E-5
19	2.718504561514729E-5	2.718504561514729E-5	0.0	3.2622054738176747E-4	8.155513684544187E-5
20	2.718504561514729E-5	2.718504561514729E-5	0.0	3.8059063861206204E-4	1.3592522807573645E-4
21	2.718504561514729E-5	2.718504561514729E-5	0.0	3.8059063861206204E-4	1.3592522807573645E-4
22	2.718504561514729E-5	2.718504561514729E-5	0.0	5.708859579180931E-4	1.3592522807573645E-4
23	2.718504561514729E-5	2.718504561514729E-5	0.0	5.980710035332404E-4	1.6311027369088373E-4
24	2.718504561514729E-5	2.718504561514729E-5	0.0	7.611812772241241E-4	1.9029531930603102E-4
25	2.718504561514729E-5	2.718504561514729E-5	0.0	8.42736414069566E-4	1.9029531930603102E-4
26	2.718504561514729E-5	2.718504561514729E-5	0.0	0.001114586870221039	1.9029531930603102E-4
27	2.718504561514729E-5	2.718504561514729E-5	0.0	0.0014679924632179536	1.9029531930603102E-4
28	2.718504561514729E-5	2.718504561514729E-5	0.0	0.0020388784211360467	1.9029531930603102E-4
29	2.718504561514729E-5	2.718504561514729E-5	0.0	0.0032350204282025276	1.9029531930603102E-4
30	2.718504561514729E-5	5.437009123029458E-5	0.0	0.005056418484417396	1.9029531930603102E-4
31	2.718504561514729E-5	5.437009123029458E-5	0.0	0.01014002201444994	1.9029531930603102E-4
32	2.718504561514729E-5	5.437009123029458E-5	0.0	0.016011991867321752	1.9029531930603102E-4
33	2.718504561514729E-5	5.437009123029458E-5	0.0	0.023786914913253878	1.9029531930603102E-4
34	2.718504561514729E-5	5.437009123029458E-5	0.0	0.034144417292625	1.9029531930603102E-4
35	5.437009123029458E-5	5.437009123029458E-5	0.0	0.04681264854928363	1.9029531930603102E-4
36	5.437009123029458E-5	5.437009123029458E-5	0.0	0.06290619555345082	1.9029531930603102E-4
37	5.437009123029458E-5	5.437009123029458E-5	0.0	0.08348527508411732	1.9029531930603102E-4
38	5.437009123029458E-5	5.437009123029458E-5	0.0	0.10754404045352269	1.9029531930603102E-4
39	5.437009123029458E-5	5.437009123029458E-5	0.0	0.1340494599282913	1.9029531930603102E-4
40	8.155513684544187E-5	5.437009123029458E-5	0.0	0.1605005093118296	1.9029531930603102E-4
41	8.155513684544187E-5	5.437009123029458E-5	0.0	0.1850486055023076	1.9029531930603102E-4
42	8.155513684544187E-5	5.437009123029458E-5	0.0	0.21060254838054607	1.9029531930603102E-4
43	8.155513684544187E-5	5.437009123029458E-5	0.0	0.24053328360282322	1.9029531930603102E-4
44	8.155513684544187E-5	5.437009123029458E-5	0.0	0.2726660075199273	1.9029531930603102E-4
45	1.0874018246058916E-4	5.437009123029458E-5	0.0	0.30895804341614896	2.1748036492117833E-4
46	1.0874018246058916E-4	5.437009123029458E-5	0.0	0.3500346473406365	2.1748036492117833E-4
47	1.0874018246058916E-4	5.437009123029458E-5	0.0	0.3918180624511179	2.446654105363256E-4
48	1.0874018246058916E-4	5.437009123029458E-5	0.0	0.43376458783529015	2.990355017666202E-4
49	1.0874018246058916E-4	5.437009123029458E-5	0.0	0.4766625898159926	2.990355017666202E-4
50	1.0874018246058916E-4	5.437009123029458E-5	0.0	0.5195062217054647	2.990355017666202E-4
51	1.0874018246058916E-4	5.437009123029458E-5	0.0	0.563491625510773	2.990355017666202E-4
52	1.0874018246058916E-4	5.437009123029458E-5	0.0	0.6073411040880056	2.990355017666202E-4
53	1.0874018246058916E-4	8.155513684544187E-5	0.0	0.6552955245531255	4.077756842272094E-4
54	1.0874018246058916E-4	8.155513684544187E-5	0.0	0.7053975636218419	4.3496072984235666E-4
55	1.0874018246058916E-4	1.0874018246058916E-4	0.0	0.7592239539398336	4.3496072984235666E-4
56	1.0874018246058916E-4	1.0874018246058916E-4	0.0	0.818378613198394	4.3496072984235666E-4
57	1.0874018246058916E-4	1.0874018246058916E-4	0.0	0.8838673880852839	4.3496072984235666E-4
58	1.0874018246058916E-4	1.0874018246058916E-4	0.0	0.951639706803846	4.3496072984235666E-4
59	1.0874018246058916E-4	1.0874018246058916E-4	0.0	1.0179984031504206	4.3496072984235666E-4
60	1.0874018246058916E-4	1.0874018246058916E-4	0.0	1.0919417272236212	4.3496072984235666E-4
61	1.0874018246058916E-4	1.0874018246058916E-4	0.0	1.1681685951284941	4.3496072984235666E-4
62	1.0874018246058916E-4	1.0874018246058916E-4	0.0	1.2428187303876888	4.3496072984235666E-4
63	1.0874018246058916E-4	1.0874018246058916E-4	0.0	1.3188281179276404	4.3496072984235666E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5690	0.0	32.690594	1
GGTATCA	3055	0.0	31.403633	1
TCAACGC	7045	0.0	25.656029	4
ATCAACG	7090	0.0	25.346546	3
CAACGCA	7230	0.0	24.905487	5
TATCAAC	7385	0.0	24.471844	2
AACGCAG	7560	0.0	23.802773	6
GTACATG	8650	0.0	21.384298	1
ACGCAGA	8720	0.0	20.440632	7
TACATGG	8745	0.0	19.956142	2
CGCAGAG	9050	0.0	19.583397	8
CGTACCG	90	0.0057410584	19.164185	5
ACATGGG	8820	0.0	18.732437	3
AGAGTAC	8690	0.0	18.650352	11
GAGTACT	5625	0.0	17.533327	12
CAGAGTA	9785	0.0	16.915447	10
GCAGAGT	10210	0.0	16.515064	9
CATGGGG	5760	0.0	16.468323	4
AGTACTT	6085	0.0	16.378342	13
GTACTTT	5990	0.0	16.062605	14
>>END_MODULE