FastQCFastQC Report
Mon 6 Feb 2017
SRR2077486_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077486_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3678493
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35150.036.037081
GTATCAA70000.033.6205371
ATCAACG93000.024.8497163
TCAACGC94150.024.5091064
TATCAAC96000.024.3584732
CAACGCA95050.024.2330785
AACGCAG100200.023.0173286
ACGCAGA116900.019.6394657
CGCAGAG118750.019.1302538
AGAGTAC118450.018.30579211
GTACATG81450.017.8039671
GAGTACT86150.017.7546612
CAGAGTA126700.017.35908110
TACATGG82850.017.3272722
CGTGCCG3451.8189894E-1217.01350210
GCAGAGT132600.016.6894869
AGTACTT91650.016.60728513
ACATGGG83700.016.5747433
ACACCGT6150.016.280556
GTACTTT93500.016.27160614
Produced by FastQC (version 0.11.5)