##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077486_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3678493 Sequences flagged as poor quality 0 Sequence length 75 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.025869289407375 33.0 31.0 34.0 28.0 34.0 2 31.12742935762009 33.0 31.0 34.0 28.0 34.0 3 31.196822448758226 33.0 31.0 34.0 27.0 34.0 4 34.58493872354793 37.0 35.0 37.0 32.0 37.0 5 34.30275196935267 37.0 35.0 37.0 30.0 37.0 6 34.28515617672781 37.0 35.0 37.0 30.0 37.0 7 34.13558432760372 37.0 35.0 37.0 30.0 37.0 8 34.09326210488915 37.0 35.0 37.0 30.0 37.0 9 35.59229662799412 39.0 37.0 39.0 30.0 39.0 10 35.49050956465052 39.0 37.0 39.0 30.0 39.0 11 35.483559979589465 39.0 37.0 39.0 30.0 39.0 12 35.37741814378877 39.0 35.0 39.0 29.0 39.0 13 35.32037467517269 39.0 35.0 39.0 29.0 39.0 14 36.579945102518884 40.0 37.0 41.0 29.0 41.0 15 36.629562704074736 40.0 37.0 41.0 29.0 41.0 16 36.61020015533535 40.0 37.0 41.0 29.0 41.0 17 36.59979100136931 40.0 37.0 41.0 29.0 41.0 18 36.60529298275136 40.0 37.0 41.0 29.0 41.0 19 36.644922526697755 40.0 37.0 41.0 29.0 41.0 20 36.61039779061697 40.0 37.0 41.0 29.0 41.0 21 36.58758545958902 40.0 37.0 41.0 29.0 41.0 22 36.56487779098669 40.0 37.0 41.0 29.0 41.0 23 36.53536570546689 40.0 37.0 41.0 29.0 41.0 24 36.51549207786993 40.0 37.0 41.0 29.0 41.0 25 36.48793378157849 40.0 37.0 41.0 29.0 41.0 26 36.41753185339758 40.0 37.0 41.0 28.0 41.0 27 36.36774842306347 40.0 37.0 41.0 28.0 41.0 28 36.34494016979236 40.0 37.0 41.0 28.0 41.0 29 36.297727629222074 40.0 36.0 41.0 28.0 41.0 30 36.22598330348868 40.0 36.0 41.0 28.0 41.0 31 36.14519016347184 40.0 36.0 41.0 28.0 41.0 32 36.08153202955667 40.0 36.0 41.0 27.0 41.0 33 36.02806285073806 40.0 36.0 41.0 27.0 41.0 34 35.96576478465502 40.0 36.0 41.0 27.0 41.0 35 35.88764175981849 40.0 36.0 41.0 27.0 41.0 36 35.83665267271135 40.0 35.0 41.0 27.0 41.0 37 35.779973755556966 40.0 35.0 41.0 27.0 41.0 38 35.72663424940594 40.0 35.0 41.0 26.0 41.0 39 35.65779872355337 40.0 35.0 41.0 26.0 41.0 40 35.59848068217066 40.0 35.0 41.0 26.0 41.0 41 35.556903057855486 40.0 35.0 41.0 26.0 41.0 42 35.50430271309474 40.0 35.0 41.0 25.0 41.0 43 35.413324423887715 40.0 35.0 41.0 25.0 41.0 44 35.30096156224845 40.0 35.0 41.0 24.0 41.0 45 35.21658162731314 40.0 35.0 41.0 24.0 41.0 46 35.10010702752459 40.0 35.0 41.0 24.0 41.0 47 34.96929748133271 40.0 35.0 41.0 23.0 41.0 48 34.88397476901547 39.0 35.0 41.0 23.0 41.0 49 34.80560898172159 39.0 35.0 41.0 23.0 41.0 50 34.81058520432144 39.0 35.0 41.0 23.0 41.0 51 34.745628168926785 39.0 35.0 41.0 22.0 41.0 52 34.68673448610613 39.0 35.0 41.0 22.0 41.0 53 34.59829283350546 39.0 35.0 41.0 22.0 41.0 54 34.57455947313207 39.0 35.0 41.0 22.0 41.0 55 34.49706605395198 39.0 34.0 41.0 21.0 41.0 56 34.38342549516881 39.0 34.0 41.0 20.0 41.0 57 34.32035319898665 39.0 34.0 41.0 20.0 41.0 58 34.22823748747109 39.0 34.0 41.0 20.0 41.0 59 34.13848415641949 39.0 34.0 41.0 19.0 41.0 60 34.11439113789261 39.0 34.0 41.0 19.0 41.0 61 34.01037272600492 38.0 34.0 41.0 18.0 41.0 62 33.87024006841932 38.0 34.0 41.0 18.0 41.0 63 33.74574887052932 38.0 34.0 41.0 17.0 41.0 64 33.59699230092323 37.0 34.0 40.0 14.0 41.0 65 33.47276072021885 37.0 34.0 40.0 13.0 41.0 66 33.28047409632151 37.0 34.0 40.0 11.0 41.0 67 33.094945131063184 37.0 34.0 40.0 8.0 41.0 68 32.88641517056033 37.0 33.0 40.0 6.0 41.0 69 32.724042155306535 36.0 33.0 39.0 2.0 41.0 70 32.53334313807312 36.0 33.0 39.0 2.0 41.0 71 32.35472026180286 36.0 33.0 39.0 2.0 41.0 72 32.149952983463606 36.0 33.0 39.0 2.0 41.0 73 31.944835833587288 35.0 33.0 39.0 2.0 41.0 74 31.681432042958896 35.0 33.0 38.0 2.0 40.0 75 31.455307920933926 35.0 33.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.43172087299718953 1101 2 0.5107993964769086 1101 3 0.5449580266763654 1101 4 0.5451719664971009 1101 5 0.5262203124057123 1101 6 0.4949599639550115 1101 7 0.5562652973376885 1101 8 0.6321926592137856 1101 9 0.7878016165940451 1101 10 0.7522547537395354 1101 11 0.728957223737801 1101 12 0.7730630859227148 1101 13 0.7465679933386866 1101 14 0.9022510658173886 1101 15 0.7963033612198771 1101 16 0.7918859953440816 1101 17 0.8031548412744911 1101 18 0.7283365511069917 1101 19 0.8993587225347071 1101 20 0.8837769774375346 1101 21 0.8366382787335525 1101 22 0.9178355600545771 1101 23 0.8122828967386511 1101 24 0.8346057667137146 1101 25 0.8966572813877534 1101 26 0.935424474362847 1101 27 0.9798898205309641 1101 28 0.8928600186609117 1101 29 0.8824907022476225 1101 30 0.8168439751785428 1101 31 0.8809433320005695 1101 32 0.8076545418708037 1101 33 0.7198621685748137 1101 34 0.7160775948146778 1101 35 0.8134425598719943 1101 36 0.774782755618368 1101 37 0.8306668760504508 1101 38 0.8729981427661073 1101 39 0.8167487364568373 1101 40 0.8346634986528514 1101 41 0.7913421454198826 1101 42 0.77074958748684 1101 43 0.8409303418672351 1101 44 0.8683478831534259 1101 45 0.8754063915277044 1101 46 0.9529820572004652 1101 47 0.8628395369121407 1101 48 0.8950585700407103 1101 49 1.0000517198265797 1101 50 0.805716812785569 1101 51 0.8669381326298335 1101 52 0.7962025050878907 1101 53 0.8532818980482375 1101 54 0.8434359629846497 1101 55 0.8163501568116942 1101 56 0.8615484801757134 1101 57 0.7698298977486573 1101 58 0.7673419109353006 1101 59 0.8528175482165423 1101 60 0.8249270820137795 1101 61 0.7743319937159541 1101 62 0.8157345270945484 1101 63 0.8636699903334488 1101 64 0.8952780734846684 1101 65 0.7652252606551357 1101 66 0.8305770519665501 1101 67 0.8318845257692189 1101 68 0.8713377898755112 1101 69 0.8515128807545764 1101 70 0.8386641549776215 1101 71 0.8723546870006018 1101 72 0.8816264850929088 1101 73 0.8484785136348449 1101 74 0.9017615904715477 1101 75 0.9570353617223013 1102 1 0.7075690577848057 1102 2 0.7215445991517342 1102 3 0.7140870966558595 1102 4 0.7635890766961069 1102 5 0.8194964496403756 1102 6 0.851955161684053 1102 7 0.868501394545639 1102 8 0.9000204292241776 1102 9 1.0114457241093788 1102 10 1.11435217103066 1102 11 1.0229559137332416 1102 12 1.0804567343789273 1102 13 1.0200070934137528 1102 14 1.1751423163753643 1102 15 1.2486639052638324 1102 16 1.2728135231726867 1102 17 1.2252323387269612 1102 18 1.2163549012422195 1102 19 1.2439927575465717 1102 20 1.1873659654859878 1102 21 1.159319665985933 1102 22 1.249769816673755 1102 23 1.22016288708447 1102 24 1.2719469754213293 1102 25 1.2586201372552424 1102 26 1.278601749776378 1102 27 1.2713353325064318 1102 28 1.3087263607879223 1102 29 1.3442547553916526 1102 30 1.2929781524492867 1102 31 1.3695509324009763 1102 32 1.3394095631053275 1102 33 1.3113906978626915 1102 34 1.307953875998777 1102 35 1.3807935918153191 1102 36 1.3195973928510583 1102 37 1.3291374942884318 1102 38 1.3529853232816436 1102 39 1.380194213901703 1102 40 1.3495139577839126 1102 41 1.3313309877204347 1102 42 1.3197964170923626 1102 43 1.4086518223810103 1102 44 1.4247473920995475 1102 45 1.428077243350799 1102 46 1.4004445798117118 1102 47 1.497554945436086 1102 48 1.471567584145376 1102 49 1.4768597473273246 1102 50 1.3614278319669566 1102 51 1.3690839575028733 1102 52 1.2629999324144663 1102 53 1.2744725346449854 1102 54 1.3874122620455225 1102 55 1.426300867980153 1102 56 1.3885818018429816 1102 57 1.4250149727609838 1102 58 1.3585322921495049 1102 59 1.3735602582138355 1102 60 1.3934421907689725 1102 61 1.4231221099078155 1102 62 1.3829254953289194 1102 63 1.361033003674848 1102 64 1.3839466060026027 1102 65 1.3454617448935409 1102 66 1.398606322403353 1102 67 1.4448842167923033 1102 68 1.5013662452463308 1102 69 1.4727317533089703 1102 70 1.5080577796940489 1102 71 1.46921999213059 1102 72 1.4697878494543843 1102 73 1.5258678781387545 1102 74 1.5643223652230596 1102 75 1.5442351568407524 1103 1 0.5169961701139307 1103 2 0.5694932306100959 1103 3 0.5728251450490802 1103 4 0.6133023611652817 1103 5 0.5945724132789891 1103 6 0.6147687092705567 1103 7 0.7210131757020051 1103 8 0.7730515347578475 1103 9 0.8929176022695486 1103 10 0.9397649173379037 1103 11 0.9744795438843923 1103 12 0.878353436133601 1103 13 0.8512179218591385 1103 14 1.0137135950627183 1103 15 0.9955261652705119 1103 16 0.9855754144596673 1103 17 1.0227753238424953 1103 18 0.9795798537467917 1103 19 1.0430889517264177 1103 20 1.1026941991481252 1103 21 1.0635269459870003 1103 22 1.0725002288886287 1103 23 1.1214860607190147 1103 24 1.0905589639889328 1103 25 1.0995315505463026 1103 26 1.1271579536415643 1103 27 1.2032848990070377 1103 28 1.115429342362873 1103 29 1.0882323622234722 1103 30 1.1223890929220204 1103 31 1.2080921024546072 1103 32 1.1543058865369815 1103 33 1.1504317532574504 1103 34 1.1687660709181742 1103 35 1.115813521270205 1103 36 1.1634032335158935 1103 37 1.2130635165893295 1103 38 1.2228919318362585 1103 39 1.2446281254506744 1103 40 1.2593901022793759 1103 41 1.1192770423445353 1103 42 1.2001161700990437 1103 43 1.2219387180168297 1103 44 1.2684499543408592 1103 45 1.2596148239494909 1103 46 1.2444572165522416 1103 47 1.2704428213625434 1103 48 1.3193404167253107 1103 49 1.2147068467312678 1103 50 1.2234319665214528 1103 51 1.2094704689716593 1103 52 1.2712407782449375 1103 53 1.2756861415602003 1103 54 1.24842646846772 1103 55 1.2720660937726862 1103 56 1.2299993545954635 1103 57 1.186761047958754 1103 58 1.2647231877224385 1103 59 1.14540314924416 1103 60 1.1536943856063502 1103 61 1.203873294687682 1103 62 1.201568993726596 1103 63 1.2163843946698023 1103 64 1.2181475532796853 1103 65 1.186510783006419 1103 66 1.2532244434263902 1103 67 1.1919072384483087 1103 68 1.1775670619729226 1103 69 1.1140416873467984 1103 70 1.1435767059265274 1103 71 1.1782710370300435 1103 72 1.1862518723711233 1103 73 1.131917902215413 1103 74 1.1907325241016373 1103 75 1.1923967017930117 1104 1 0.4663998390651365 1104 2 0.4708118415815008 1104 3 0.45984715353186445 1104 4 0.5498719423794896 1104 5 0.5454152853630134 1104 6 0.5977516904661826 1104 7 0.555628912598813 1104 8 0.6433869542330655 1104 9 0.7358508916910651 1104 10 0.6750946180266411 1104 11 0.6783449740102085 1104 12 0.7483449017664938 1104 13 0.6878327375336681 1104 14 0.8080603125718611 1104 15 0.7424271252793488 1104 16 0.7700093041967051 1104 17 0.8136618365995716 1104 18 0.7475576253742702 1104 19 0.7880292227297687 1104 20 0.8111206053595339 1104 21 0.830105921287597 1104 22 0.8785595964725204 1104 23 0.8656152857845925 1104 24 0.8720863081871286 1104 25 0.8817172743786799 1104 26 0.9109856090250759 1104 27 1.051737817638248 1104 28 0.9857538500165219 1104 29 0.9296342270868436 1104 30 0.865396483531832 1104 31 0.8919440945068331 1104 32 0.914694552394856 1104 33 0.8791952487834394 1104 34 0.8728023537168781 1104 35 1.0125273435391193 1104 36 1.0265103317219584 1104 37 0.9627802225550823 1104 38 0.9621254854891887 1104 39 0.9450376494208044 1104 40 0.9594218788264328 1104 41 0.9872441005472012 1104 42 0.9744007431310351 1104 43 0.9752114543677095 1104 44 1.0107833581050372 1104 45 0.992553942929014 1104 46 0.9889737451512985 1104 47 0.9952347775644768 1104 48 0.9550105993131552 1104 49 1.0762807348083854 1104 50 0.9955707463747885 1104 51 1.0124761136900275 1104 52 1.0206036105305714 1104 53 0.9522699676614721 1104 54 1.0552066445316157 1104 55 1.0275037685891633 1104 56 1.0316015075970526 1104 57 1.0424258539945086 1104 58 1.050228882866648 1104 59 1.0379701486552548 1104 60 0.9365831614090894 1104 61 0.9945328415561008 1104 62 0.9989996896065279 1104 63 0.9458549762335196 1104 64 0.9703109872365445 1104 65 0.943011236177675 1104 66 1.0268688562596182 1104 67 1.0622587941929993 1104 68 1.0573846559956266 1104 69 0.9755104251433337 1104 70 0.967762626171691 1104 71 0.930100007122725 1104 72 0.924044767981691 1104 73 0.9343023005590609 1104 74 1.0503972401975368 1104 75 1.0414462325350833 1105 1 0.3643805332151224 1105 2 0.4050596201355532 1105 3 0.3620516369565365 1105 4 0.34403377210798425 1105 5 0.3493451112815862 1105 6 0.489529983431936 1105 7 0.49659997477512263 1105 8 0.5231785045593824 1105 9 0.658471968251277 1105 10 0.6891837466203015 1105 11 0.6150822547363504 1105 12 0.7140593302261422 1105 13 0.7078051623526207 1105 14 0.7430421936955938 1105 15 0.6966540975454194 1105 16 0.7320714313535817 1105 17 0.7839291514683353 1105 18 0.7146662846418934 1105 19 0.8583278840199995 1105 20 0.7724771559960857 1105 21 0.8177520467812585 1105 22 0.7668456241073329 1105 23 0.7615467781401719 1105 24 0.7934122698735848 1105 25 0.8508224347732423 1105 26 0.8177445905000624 1105 27 0.8159250548903643 1105 28 0.8554913378385578 1105 29 0.8699857743832808 1105 30 0.8292046627721206 1105 31 0.8871997451223592 1105 32 0.8290627366634595 1105 33 0.7775008498007097 1105 34 0.9084399310655797 1105 35 0.9011010491293376 1105 36 0.9477985810041716 1105 37 0.9244271909371022 1105 38 0.8582945337586452 1105 39 0.9330718581170601 1105 40 0.8771637116331661 1105 41 0.8096338260121811 1105 42 0.8599410176418871 1105 43 0.8593338069448464 1105 44 0.9261086960863452 1105 45 0.9575334629983843 1105 46 0.8703290709405422 1105 47 0.8941550585380043 1105 48 0.8921802833750689 1105 49 0.9057462098275479 1105 50 0.9249410539806888 1105 51 0.8949902095069291 1105 52 0.8171453508290938 1105 53 0.7713635645303754 1105 54 0.9388585590404972 1105 55 0.8533330587176167 1105 56 0.9221983063941295 1105 57 0.8523558201258723 1105 58 0.9053848857063542 1105 59 0.9003551681279092 1105 60 0.8309507171376325 1105 61 0.8698490254153555 1105 62 0.9282936054192206 1105 63 0.8876566782877973 1105 64 0.8973611761611835 1105 65 0.8531717475836658 1105 66 0.8738963855088926 1105 67 0.9381308331044664 1105 68 0.9880885967001234 1105 69 0.9646677865965785 1105 70 0.958394961657369 1105 71 0.9056546332985178 1105 72 0.8941812175844603 1105 73 0.9273243776261353 1105 74 0.9727878520651778 1105 75 1.037401818926707 1106 1 0.32853759847016306 1106 2 0.2960983602154563 1106 3 0.2796049605474735 1106 4 0.26879021615573606 1106 5 0.2814398314908999 1106 6 0.27818036009755787 1106 7 0.37045600127594724 1106 8 0.372676658106883 1106 9 0.4849011605011526 1106 10 0.5193038960204461 1106 11 0.5251246974605053 1106 12 0.5167709897305315 1106 13 0.492105485742961 1106 14 0.5247563997005997 1106 15 0.5605378196556146 1106 16 0.5926080547952424 1106 17 0.6304555446096387 1106 18 0.5714848695252215 1106 19 0.5918268160794398 1106 20 0.6327333517714209 1106 21 0.6319014227385935 1106 22 0.6913150429299435 1106 23 0.6738208922568916 1106 24 0.6249423490081654 1106 25 0.6146989781111145 1106 26 0.661063219131492 1106 27 0.6937992460414364 1106 28 0.6123669601886377 1106 29 0.5858916875396147 1106 30 0.5060045828474102 1106 31 0.6140238012669599 1106 32 0.6492565446660166 1106 33 0.687916840021245 1106 34 0.6354196267641754 1106 35 0.671061578448338 1106 36 0.6616431026191094 1106 37 0.6316138646223166 1106 38 0.5741187314365916 1106 39 0.6501014612063472 1106 40 0.6101381377672865 1106 41 0.536440634253033 1106 42 0.6277601678731699 1106 43 0.6501409570020797 1106 44 0.6957660204526519 1106 45 0.6295259933051298 1106 46 0.5796517514155823 1106 47 0.7216263823271873 1106 48 0.6455954861298281 1106 49 0.671133060044582 1106 50 0.6971951774942156 1106 51 0.6816339857862275 1106 52 0.6091842022820728 1106 53 0.6163772945215129 1106 54 0.6727446107172881 1106 55 0.6421922638280222 1106 56 0.6477801992997243 1106 57 0.6300735077569044 1106 58 0.7030258185948881 1106 59 0.6460642336920941 1106 60 0.6106139306105973 1106 61 0.597361960950451 1106 62 0.6308841054686241 1106 63 0.6717568757276027 1106 64 0.6388425260405697 1106 65 0.6167469395686993 1106 66 0.5590366880949347 1106 67 0.5668767738385938 1106 68 0.6010357403577231 1106 69 0.567271026217874 1106 70 0.6100641204733392 1106 71 0.5519486667019606 1106 72 0.5836458743833788 1106 73 0.5144395023569075 1106 74 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-1.6588966040932966 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 73562.0 3 29727.0 4 17315.0 5 11013.0 6 8122.0 7 7277.0 8 6851.0 9 7145.0 10 7578.0 11 8309.0 12 8778.0 13 9365.0 14 9844.0 15 10364.0 16 11250.0 17 11912.0 18 12768.0 19 13865.0 20 15824.0 21 17970.0 22 22161.0 23 26848.0 24 28278.0 25 29628.0 26 31796.0 27 34196.0 28 38885.0 29 46967.0 30 54254.0 31 62277.0 32 74685.0 33 93646.0 34 121658.0 35 163872.0 36 230058.0 37 355568.0 38 609962.0 39 1048906.0 40 306009.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.22631877203802 18.56176795532013 14.107111230935518 26.104802041706332 2 14.842733573563155 19.860370154766336 38.64729203095163 26.649604240718872 3 17.88052842568966 23.900322912311285 29.53217677913048 28.686971882868573 4 11.92137749186653 16.287168212781562 37.35151702740866 34.43993726794325 5 13.395190782010182 37.56822292627747 34.91313099318866 14.123455298523687 6 32.55080652510708 37.239492866479594 16.856789596172966 13.352911012240357 7 28.15040233118501 31.979339992489347 21.224394232623048 18.645863443702595 8 26.205203325163907 35.41471959599003 19.492387967694647 18.887689111151413 9 25.810990170640295 15.587395355668338 19.31178163264196 39.28983284104941 10 13.643967736268852 29.600983509733126 34.24380316535244 22.511245588645586 11 35.03077289505568 23.13375088909142 23.185002795064154 18.650473420788753 12 22.849684095027566 25.049657371619467 30.657246166401926 21.443412366951037 13 27.836891637932045 21.994591460449882 26.02743341426099 24.141083487357083 14 21.33020991676601 21.339239647699067 27.864226572267903 29.46632386326703 15 23.298469891331123 29.407756531253384 24.019774983699396 23.273998593716097 16 22.859038647710832 28.59397604777392 25.82720568239914 22.719779622116107 17 21.797002096145338 28.149771746114354 27.17049201634825 22.882734141392056 18 21.694335321474096 27.690298134105966 28.305583108199233 22.309783436220705 19 22.51821912102325 27.251683249936647 28.586619264144357 21.643478364895742 20 22.591035592053533 26.817340454571653 28.64642638468825 21.945197568686563 21 22.842594042864228 26.47688412039575 28.557498194411814 22.123023642328207 22 22.37413013888957 26.611401223420195 28.386660162930553 22.627808474759686 23 22.384624168701297 26.51395603021224 28.15431296526898 22.947106835817486 24 22.109011768998833 26.60745958153885 28.544614547138398 22.738914102323918 25 22.542741869046107 26.49382767742148 28.396408761291276 22.567021692241145 26 22.292724978516496 26.71566174630973 28.44910857055835 22.542504704615418 27 22.413665108045233 26.923107253912075 28.120041496766053 22.54318614127664 28 22.24817254632781 26.761579946865893 28.368276073254243 22.621971433552048 29 22.352503758173533 26.81860376815544 28.40556342956488 22.423329044106147 30 22.620225398657354 26.807766088385293 28.255191832521216 22.316816680436133 31 22.55553437724576 26.46377543765007 28.348696369291478 22.63199381581269 32 22.32085446638672 26.804655139834992 28.218651825610337 22.655838568167947 33 22.35933213521516 26.649175228861417 28.146623563413716 22.844869072509713 34 22.47616774125489 26.789139091429632 28.358661423959447 22.37603174335603 35 22.671458748494544 26.726576751697117 28.169856101612435 22.4321083981959 36 22.456730273841544 26.76767580649941 28.19263167424702 22.582962245412027 37 22.5975011772024 26.48952806417901 28.21185945780178 22.70111130081681 38 22.41910087189893 26.693205321432533 28.299552415066522 22.58814139160202 39 22.539501118349882 26.98274992543881 28.00556361055052 22.472185345660783 40 22.7213247306823 26.545971809832874 28.31846737886583 22.414236080618995 41 22.549014010295043 26.693838444558455 28.05683180984867 22.700315735297835 42 22.6097096015068 26.720951450904995 27.97431717640099 22.695021771187214 43 22.460571579890928 26.55279693082419 28.118025480945295 22.868606008339583 44 22.544271943485032 26.876609493401077 27.916608666904462 22.662509896209425 45 22.679039035381525 26.778618624235516 27.80186194507034 22.740480395312623 46 22.632398664969198 26.676910203492426 27.675247259894675 23.015443871643697 47 22.584843265062887 26.80479524303539 27.64683909358423 22.96352239831749 48 22.404976672527194 26.97512778387513 27.68904822660368 22.930847316994 49 22.677787709545807 26.83298209671947 27.557013423110714 22.932216770624006 50 22.73626852801317 26.868351201201364 27.591692664695564 22.803687606089902 51 22.545030252152014 27.129721053844918 27.287914145653996 23.037334548349072 52 22.627234390529814 27.120183514172435 27.331144342878765 22.92143775241898 53 22.638237995831446 26.99352696878858 27.466356467172837 22.90187856820714 54 22.603969943109824 27.062564513546448 27.510927714652556 22.822537828691168 55 22.59111493152346 26.851155611303128 27.4667349556312 23.090994501542212 56 22.570297349489437 27.197455709957897 27.39146380685972 22.84078313369295 57 22.697578395753794 26.879845193057395 27.2763504013171 23.146226009871715 58 22.47063393830225 26.875984951397058 27.533022462260913 23.120358648039772 59 22.64142633433381 27.08230023054504 27.375048936447865 22.90122449867328 60 22.578435253128493 27.21435779437415 27.475065571132173 22.732141381365185 61 22.799207279113325 26.84632469477799 27.433905944591093 22.920562081517588 62 22.5095870088179 27.206466768163107 27.443548608575135 22.840397614443855 63 22.420931662107797 26.853315186672532 27.520873780204823 23.20487937101484 64 22.473396301311016 26.919762048471206 27.56586352588049 23.040978124337286 65 22.55113188657914 26.86550972010217 27.39715300439769 23.186205388921003 66 22.394744748623175 27.086160509755032 27.380544857110618 23.138549884511175 67 22.495157531140254 27.029133119224248 27.303884089621295 23.171825260014202 68 22.54593970848297 26.916356885409577 27.390953009681706 23.14675039642575 69 22.511282569924745 27.08287115577015 27.23848905998521 23.167357214319892 70 22.506235108944818 26.74235711452236 27.41000929046866 23.34139848606416 71 22.45742701686933 26.819599626231543 27.38704909150177 23.335924265397352 72 22.378135554602114 27.027285694165528 27.288875330419714 23.305703420812648 73 22.56044695471321 26.722508818791674 27.376873356034498 23.340170870460618 74 22.463893623737803 26.917627405199774 27.197522005604963 23.42095696545746 75 22.474430105965915 26.95871382678614 27.176360023673034 23.39049604357491 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 985.0 1 659.5 2 402.0 3 500.0 4 530.0 5 404.0 6 374.5 7 780.0 8 1089.0 9 1865.0 10 3061.0 11 3410.0 12 3339.0 13 3058.5 14 2961.5 15 3496.5 16 3848.0 17 4274.0 18 5224.0 19 6720.0 20 7692.0 21 8844.5 22 11881.5 23 16025.5 24 18285.0 25 21620.5 26 29483.5 27 38595.5 28 43180.0 29 49402.5 30 61185.5 31 73147.5 32 79549.0 33 85592.5 34 98383.0 35 111433.0 36 117736.0 37 122764.5 38 132581.0 39 142820.0 40 148271.0 41 153639.5 42 162673.5 43 170130.5 44 173922.0 45 177635.0 46 182106.0 47 183852.5 48 184841.0 49 185178.0 50 182164.0 51 175049.0 52 171285.0 53 166664.0 54 156428.0 55 142523.5 56 134234.0 57 126938.5 58 111592.0 59 93259.0 60 82977.0 61 74112.0 62 57069.5 63 41814.0 64 34736.0 65 29358.5 66 20279.5 67 14200.5 68 11823.0 69 9827.0 70 6613.0 71 4464.0 72 3533.0 73 3223.0 74 2543.5 75 1629.5 76 1085.0 77 896.5 78 546.5 79 300.5 80 216.0 81 159.0 82 76.5 83 43.0 84 35.0 85 25.0 86 9.5 87 8.0 88 12.0 89 7.5 90 4.0 91 3.0 92 1.0 93 1.5 94 1.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07459576516796416 2 0.017017838555082203 3 0.0025825793334389923 4 0.13842625227233 5 0.09764868384960906 6 0.06899564577124381 7 0.028544297895904652 8 0.026097643790541396 9 0.0476553849633532 10 0.0285714829415198 11 0.015794511502400573 12 0.10362939388494147 13 0.08682903569478044 14 0.047573829826507756 15 0.019437307614830313 16 0.03188805850656777 17 0.02147618603596636 18 0.018621756246375895 19 0.013293487305807025 20 0.013076006940885846 21 0.027755931573065384 22 0.016501322688394406 23 0.01394592840057056 24 0.02120433557981489 25 0.01473429472340983 26 0.034062862155779555 27 0.029849180085431724 28 0.03756973304013356 29 0.05002048393187101 30 0.0346609331593128 31 0.01973634311659693 32 0.011091498610980095 33 0.013674077944419088 34 0.013456597579497909 35 0.0055185642598749 36 0.009351655691610667 37 0.007638997817856388 38 0.0023107288772875197 39 0.00831862395823507 40 0.010928388337289211 41 0.011200238793440684 42 0.006442855810789907 43 0.010112836968834792 44 0.008617659460001691 45 0.005137973621262838 46 0.002066063466751194 47 0.0049204932563416594 48 0.005763229670411226 49 0.0016582877825239847 50 2.446654105363256E-4 51 0.0020932485123663414 52 0.0023107288772875197 53 0.0 54 5.4370091230294576E-5 55 1.9029531930603102E-4 56 0.008046773502083597 57 0.0028544297895904654 58 3.2622054738176747E-4 59 0.006877816540632264 60 0.001223327052681628 61 6.252560491483876E-4 62 0.02337913922902667 63 0.00853610432315625 64 0.011771124751358776 65 0.017697464695460886 66 0.017615909558615445 67 0.016773173144545878 68 0.017099393691927645 69 0.006877816540632264 70 0.014408074176028064 71 0.007611812772241242 72 0.016175102141012636 73 0.0072855922248594735 74 0.018485831018300158 75 0.0012505120982967753 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 3678493.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.79124233969387 #Duplication Level Percentage of deduplicated Percentage of total 1 76.35823188841748 47.946282431361624 2 13.307403133019537 16.711767500748625 3 4.683708644259547 8.822876535906609 4 2.1293327039828998 5.348137833505036 5 1.1434790693766093 3.590023567779715 6 0.6645428301411791 2.5036481935500476 7 0.3999561938114446 1.7579622403613195 8 0.26967470083707373 1.3546567594516115 9 0.19054531645396916 1.076811942793935 >10 0.8117249571311642 8.477937961458005 >50 0.03200658907822396 1.356976680401404 >100 0.009177134449422615 0.9004237199371301 >500 8.673562710971937E-5 0.029327990275123613 >1k 1.3010341430404654E-4 0.12316664246975836 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.718504561514729E-5 0.0 3 0.0 0.0 0.0 2.718504561514729E-5 0.0 4 0.0 0.0 0.0 5.437009123029458E-5 0.0 5 0.0 0.0 0.0 5.437009123029458E-5 0.0 6 0.0 0.0 0.0 5.437009123029458E-5 0.0 7 0.0 0.0 0.0 5.437009123029458E-5 0.0 8 0.0 0.0 0.0 5.437009123029458E-5 0.0 9 0.0 0.0 0.0 8.155513684544187E-5 0.0 10 0.0 0.0 0.0 1.0874018246058916E-4 0.0 11 0.0 0.0 0.0 1.0874018246058916E-4 0.0 12 0.0 0.0 0.0 1.0874018246058916E-4 2.718504561514729E-5 13 0.0 0.0 0.0 1.0874018246058916E-4 5.437009123029458E-5 14 0.0 0.0 0.0 1.3592522807573645E-4 8.155513684544187E-5 15 0.0 0.0 0.0 1.3592522807573645E-4 1.3592522807573645E-4 16 0.0 0.0 0.0 1.6311027369088373E-4 1.6311027369088373E-4 17 0.0 0.0 0.0 1.9029531930603102E-4 1.6311027369088373E-4 18 0.0 0.0 0.0 2.1748036492117833E-4 1.9029531930603102E-4 19 0.0 0.0 0.0 2.1748036492117833E-4 1.9029531930603102E-4 20 0.0 0.0 0.0 2.718504561514729E-4 2.1748036492117833E-4 21 0.0 0.0 0.0 2.718504561514729E-4 2.1748036492117833E-4 22 0.0 0.0 0.0 4.6214577545750394E-4 2.1748036492117833E-4 23 0.0 0.0 0.0 5.165158666877985E-4 2.446654105363256E-4 24 0.0 0.0 0.0 6.524410947635349E-4 2.446654105363256E-4 25 0.0 0.0 0.0 7.068111859938295E-4 2.446654105363256E-4 26 0.0 0.0 0.0 9.514765965301552E-4 2.718504561514729E-4 27 0.0 0.0 0.0 0.0013048821895270699 2.718504561514729E-4 28 0.0 0.0 0.0 0.001875768147445163 2.718504561514729E-4 29 0.0 0.0 0.0 0.0031534652913570854 2.718504561514729E-4 30 0.0 0.0 0.0 0.005056418484417396 2.718504561514729E-4 31 0.0 0.0 0.0 0.01003128183198935 2.718504561514729E-4 32 0.0 0.0 0.0 0.01593043673047631 2.718504561514729E-4 33 0.0 0.0 0.0 0.02397721023255991 2.718504561514729E-4 34 0.0 0.0 0.0 0.03430752756631588 2.718504561514729E-4 35 0.0 2.718504561514729E-5 0.0 0.04689420368612907 2.718504561514729E-4 36 0.0 2.718504561514729E-5 0.0 0.06301493573591142 2.718504561514729E-4 37 0.0 2.718504561514729E-5 0.0 0.08353964517534762 2.990355017666202E-4 38 0.0 2.718504561514729E-5 0.0 0.10719063486052577 2.990355017666202E-4 39 0.0 2.718504561514729E-5 0.0 0.1338863496546004 2.990355017666202E-4 40 0.0 2.718504561514729E-5 0.0 0.16014710371883267 2.990355017666202E-4 41 0.0 2.718504561514729E-5 0.0 0.18450490459000465 2.990355017666202E-4 42 0.0 2.718504561514729E-5 0.0 0.2097054418752462 2.990355017666202E-4 43 0.0 2.718504561514729E-5 0.0 0.23952743691506276 3.2622054738176747E-4 44 0.0 2.718504561514729E-5 0.0 0.2720951215620092 3.2622054738176747E-4 45 0.0 2.718504561514729E-5 0.0 0.3085774527775369 3.5340559299691475E-4 46 0.0 2.718504561514729E-5 0.0 0.3493550212002578 3.5340559299691475E-4 47 0.0 2.718504561514729E-5 0.0 0.391464656858121 3.5340559299691475E-4 48 0.0 2.718504561514729E-5 0.0 0.4335742925159841 3.8059063861206204E-4 49 0.0 2.718504561514729E-5 0.0 0.47647229449668654 3.8059063861206204E-4 50 0.0 2.718504561514729E-5 0.0 0.5194518516142345 4.077756842272094E-4 51 0.0 2.718504561514729E-5 0.0 0.5634644404651579 4.077756842272094E-4 52 0.0 2.718504561514729E-5 0.0 0.6073682891336207 4.077756842272094E-4 53 0.0 2.718504561514729E-5 0.0 0.6552683395075103 4.6214577545750394E-4 54 0.0 2.718504561514729E-5 0.0 0.7054519337130721 4.6214577545750394E-4 55 0.0 2.718504561514729E-5 0.0 0.759495804395985 4.6214577545750394E-4 56 0.0 2.718504561514729E-5 0.0 0.8196291252966907 4.6214577545750394E-4 57 0.0 2.718504561514729E-5 0.0 0.8862324870538016 4.6214577545750394E-4 58 0.0 2.718504561514729E-5 0.0 0.9548203571408183 4.6214577545750394E-4 59 0.0 2.718504561514729E-5 0.0 1.02158682917162 4.6214577545750394E-4 60 0.0 2.718504561514729E-5 0.0 1.097052515799269 4.893308210726512E-4 61 0.0 2.718504561514729E-5 0.0 1.1732250136129116 4.893308210726512E-4 62 0.0 2.718504561514729E-5 0.0 1.2483644796931788 5.165158666877985E-4 63 0.0 2.718504561514729E-5 0.0 1.3247544578717425 5.165158666877985E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3515 0.0 36.03708 1 GTATCAA 7000 0.0 33.620537 1 ATCAACG 9300 0.0 24.849716 3 TCAACGC 9415 0.0 24.509106 4 TATCAAC 9600 0.0 24.358473 2 CAACGCA 9505 0.0 24.233078 5 AACGCAG 10020 0.0 23.017328 6 ACGCAGA 11690 0.0 19.639465 7 CGCAGAG 11875 0.0 19.130253 8 AGAGTAC 11845 0.0 18.305792 11 GTACATG 8145 0.0 17.803967 1 GAGTACT 8615 0.0 17.75466 12 CAGAGTA 12670 0.0 17.359081 10 TACATGG 8285 0.0 17.327272 2 CGTGCCG 345 1.8189894E-12 17.013502 10 GCAGAGT 13260 0.0 16.689486 9 AGTACTT 9165 0.0 16.607285 13 ACATGGG 8370 0.0 16.574743 3 ACACCGT 615 0.0 16.28055 6 GTACTTT 9350 0.0 16.271606 14 >>END_MODULE