FastQCFastQC Report
Mon 6 Feb 2017
SRR2077485_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077485_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3280058
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54050.035.82661
GGTATCA25850.035.6524281
TCAACGC68450.027.8713384
ATCAACG69750.027.35023
CAACGCA69700.027.27255
TATCAAC71750.026.8274022
CGCGTAA658.688345E-426.52389110
AACGCAG74150.025.757266
ACGCAGA86350.021.8809247
AGAGTAC83200.021.30395311
CGCAGAG88950.021.242328
CAGAGTA92200.019.74620210
GAGTACT44700.019.67310512
GTACATG84800.019.416051
GCAGAGT98700.018.4804489
TACATGG87150.018.0890242
ATACCGT2156.658411E-817.638246
AGTACTT51450.017.09286913
GTACTTT50950.016.78730414
ACATGGG92900.016.6728653
Produced by FastQC (version 0.11.5)