FastQCFastQC Report
Mon 6 Feb 2017
SRR2077485_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077485_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3280058
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32900.040.1587871
GTATCAA63550.033.634581
TCAACGC87950.023.665074
TATCAAC89400.023.6639922
ATCAACG88250.023.5447243
CAACGCA89500.023.0717895
AACGCAG94350.021.9610066
CGTGCCG4500.019.95218510
ACGCAGA110100.018.8174347
GTACATG78950.018.793961
CGCAGAG112500.018.294068
GTGGTAT15650.017.9012681
GAGTACT75550.017.8262412
AGAGTAC111500.017.74636511
TACATGG82300.017.725022
CGCACGA3650.017.00771735
AGTACTT79800.016.82458313
CAGAGTA120550.016.61461810
ACATGGG84000.016.4632113
GCAGAGT124550.016.3857659
Produced by FastQC (version 0.11.5)