##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077485_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3280058 Sequences flagged as poor quality 0 Sequence length 75 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.82874540633123 31.0 31.0 34.0 27.0 34.0 2 30.93976630901039 33.0 31.0 34.0 27.0 34.0 3 31.017314632851004 33.0 31.0 34.0 27.0 34.0 4 34.380501503327075 37.0 35.0 37.0 31.0 37.0 5 34.090277976791874 37.0 35.0 37.0 30.0 37.0 6 34.07287645523341 37.0 35.0 37.0 30.0 37.0 7 33.912374110457804 37.0 35.0 37.0 29.0 37.0 8 33.85647144044404 37.0 35.0 37.0 29.0 37.0 9 35.33875041234027 39.0 35.0 39.0 29.0 39.0 10 35.22413079280915 39.0 35.0 39.0 28.0 39.0 11 35.22361860674415 39.0 35.0 39.0 28.0 39.0 12 35.115230584337226 39.0 35.0 39.0 27.0 39.0 13 35.06135043953491 39.0 35.0 39.0 27.0 39.0 14 36.30230593483408 40.0 37.0 41.0 27.0 41.0 15 36.33687605524049 40.0 37.0 41.0 27.0 41.0 16 36.312080457113865 40.0 37.0 41.0 27.0 41.0 17 36.299061480010415 40.0 37.0 41.0 27.0 41.0 18 36.298677035589 40.0 37.0 41.0 27.0 41.0 19 36.32746158756949 40.0 37.0 41.0 27.0 41.0 20 36.29255244876767 40.0 37.0 41.0 27.0 41.0 21 36.26414289015621 40.0 37.0 41.0 27.0 41.0 22 36.24233595869342 40.0 37.0 41.0 27.0 41.0 23 36.20912496059521 40.0 37.0 41.0 27.0 41.0 24 36.19009358980847 40.0 37.0 41.0 27.0 41.0 25 36.15639875880244 40.0 36.0 41.0 27.0 41.0 26 36.08447868909635 40.0 36.0 41.0 27.0 41.0 27 36.03097079380913 40.0 36.0 41.0 27.0 41.0 28 36.009595562029695 40.0 36.0 41.0 27.0 41.0 29 35.95679405669046 40.0 36.0 41.0 27.0 41.0 30 35.88092283734007 40.0 36.0 41.0 27.0 41.0 31 35.79844441775115 40.0 36.0 41.0 27.0 41.0 32 35.73145993150121 40.0 36.0 41.0 26.0 41.0 33 35.670074126738 40.0 35.0 41.0 26.0 41.0 34 35.61181113260802 40.0 35.0 41.0 26.0 41.0 35 35.526216609584345 40.0 35.0 41.0 25.0 41.0 36 35.47268523910248 40.0 35.0 41.0 25.0 41.0 37 35.412128992840984 40.0 35.0 41.0 25.0 41.0 38 35.357083319868124 40.0 35.0 41.0 25.0 41.0 39 35.27519330450864 40.0 35.0 41.0 24.0 41.0 40 35.208931061584885 40.0 35.0 41.0 24.0 41.0 41 35.16324863767653 40.0 35.0 41.0 24.0 41.0 42 35.101228697785224 40.0 35.0 41.0 24.0 41.0 43 35.004540773364376 40.0 35.0 41.0 24.0 41.0 44 34.88159752053165 39.0 35.0 41.0 23.0 41.0 45 34.787979968646894 39.0 35.0 41.0 23.0 41.0 46 34.66051514942723 39.0 35.0 41.0 22.0 41.0 47 34.51832406622078 39.0 34.0 41.0 20.0 41.0 48 34.41680451992008 39.0 34.0 41.0 19.0 41.0 49 34.32532168638481 39.0 34.0 41.0 18.0 41.0 50 34.31689988408741 39.0 34.0 41.0 18.0 41.0 51 34.23696776093593 39.0 34.0 41.0 18.0 41.0 52 34.164975741282625 39.0 34.0 41.0 17.0 41.0 53 34.06112056555097 39.0 34.0 41.0 15.0 41.0 54 34.01794968259708 39.0 34.0 41.0 15.0 41.0 55 33.929767095581845 39.0 34.0 41.0 14.0 41.0 56 33.797675833780985 38.0 34.0 41.0 13.0 41.0 57 33.71541173967046 38.0 34.0 41.0 13.0 41.0 58 33.60796729813924 38.0 34.0 41.0 12.0 41.0 59 33.507473648331825 38.0 33.0 40.0 9.0 41.0 60 33.468946280827964 38.0 34.0 40.0 8.0 41.0 61 33.34857615322656 37.0 34.0 40.0 8.0 41.0 62 33.1962614685472 37.0 33.0 40.0 7.0 41.0 63 33.05366520957861 37.0 33.0 40.0 2.0 41.0 64 32.89062175120074 37.0 33.0 40.0 2.0 41.0 65 32.754077214488284 36.0 33.0 40.0 2.0 41.0 66 32.549743937454764 36.0 33.0 39.0 2.0 41.0 67 32.35188737516227 36.0 33.0 39.0 2.0 41.0 68 32.13415189609452 36.0 33.0 39.0 2.0 41.0 69 31.96863348148112 35.0 33.0 39.0 2.0 41.0 70 31.77742375287266 35.0 32.0 39.0 2.0 41.0 71 31.59837539458144 35.0 32.0 39.0 2.0 41.0 72 31.40425504670954 35.0 32.0 38.0 2.0 40.0 73 31.20813351471224 35.0 32.0 37.0 2.0 40.0 74 30.95234718410467 35.0 32.0 37.0 2.0 39.0 75 30.738784192230746 35.0 32.0 37.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6289822559016436 1101 2 0.7504666947946248 1101 3 0.6860967705381462 1101 4 0.6546904054615936 1101 5 0.7592142844202243 1101 6 0.7654616931211748 1101 7 0.8159317198579288 1101 8 0.8325524232870833 1101 9 0.9960648006631914 1101 10 0.9749394374143776 1101 11 1.1037329258830653 1101 12 1.1184713075897719 1101 13 1.067325757588577 1101 14 1.0999375664861546 1101 15 1.1628679739725527 1101 16 1.1045133055294158 1101 17 1.1978542890574317 1101 18 1.2195895342571248 1101 19 1.1958897166445297 1101 20 1.2182243219937234 1101 21 1.1406597010407467 1101 22 1.176857913330963 1101 23 1.183777115676584 1101 24 1.2531191069489225 1101 25 1.2559560284898836 1101 26 1.2920561113860387 1101 27 1.316333872715326 1101 28 1.2361120938730181 1101 29 1.222345463796188 1101 30 1.2151545920843105 1101 31 1.3197444060778736 1101 32 1.3256482628001862 1101 33 1.3377283706674987 1101 34 1.3073142430252034 1101 35 1.43517451390926 1101 36 1.2695048084329699 1101 37 1.3471734547026628 1101 38 1.362932883226307 1101 39 1.4347261644199776 1101 40 1.2995073944041664 1101 41 1.3359361816506308 1101 42 1.325106403320497 1101 43 1.2985170537338817 1101 44 1.3211938082805403 1101 45 1.3635380394706687 1101 46 1.358074238607422 1101 47 1.3753737484512598 1101 48 1.4389843321667541 1101 49 1.4405322105692377 1101 50 1.2814114374039107 1101 51 1.303472366505595 1101 52 1.2900781656654772 1101 53 1.295742954382284 1101 54 1.2233971616533523 1101 55 1.3271736362574629 1101 56 1.352805576133406 1101 57 1.2709223300614383 1101 58 1.2921804316323602 1101 59 1.3148845382524783 1101 60 1.3726678791747418 1101 61 1.2985232755442908 1101 62 1.3555769762851924 1101 63 1.3944351179949024 1101 64 1.367671242188436 1101 65 1.273860441407166 1101 66 1.3110386959655074 1101 67 1.3471736212837087 1101 68 1.4364651238894695 1101 69 1.3720027043023073 1101 70 1.2805904602438822 1101 71 1.305018419913356 1101 72 1.2301840373082058 1101 73 1.1586068921833608 1101 74 1.2402034950129526 1101 75 1.3153762150987163 1102 1 0.804009296853458 1102 2 0.8068048802558252 1102 3 0.8359077045818815 1102 4 0.8567343412257813 1102 5 0.8584359499521668 1102 6 0.9377740449830227 1102 7 1.005630441455665 1102 8 0.9873864149729812 1102 9 1.1810461871657054 1102 10 1.2209292457211305 1102 11 1.1869330261014426 1102 12 1.2294049805402736 1102 13 1.276263723543991 1102 14 1.4254177089106648 1102 15 1.4114463155738406 1102 16 1.3767568058133364 1102 17 1.4043777266677822 1102 18 1.4256394682923883 1102 19 1.48230610104239 1102 20 1.439375784518127 1102 21 1.4364617146602754 1102 22 1.481260459259083 1102 23 1.5102384306066483 1102 24 1.5026382759203543 1102 25 1.5733791061192193 1102 26 1.5513902718682218 1102 27 1.600770255587662 1102 28 1.577641478197421 1102 29 1.570927654517618 1102 30 1.5396803999051514 1102 31 1.6185900055094748 1102 32 1.5700516332875338 1102 33 1.6582958528061624 1102 34 1.5751191162938056 1102 35 1.6432339358491035 1102 36 1.670648191336376 1102 37 1.6773836449127657 1102 38 1.6674085271240742 1102 39 1.7707563390499672 1102 40 1.831632098127855 1102 41 1.8083032510506172 1102 42 1.8004048072422236 1102 43 1.7268633869702228 1102 44 1.7725242884388521 1102 45 1.7505131730951717 1102 46 1.7343294843578576 1102 47 1.8417061302161812 1102 48 1.8031527668390694 1102 49 1.8262473753256998 1102 50 1.7881023293968923 1102 51 1.79724464902678 1102 52 1.8268198271999836 1102 53 1.811059406793028 1102 54 1.8062134092485351 1102 55 1.7914617909257018 1102 56 1.760484738550737 1102 57 1.7609656891638608 1102 58 1.7368150759736594 1102 59 1.7979906087033868 1102 60 1.773127910829345 1102 61 1.747226289793808 1102 62 1.7255420887783757 1102 63 1.7285984693567116 1102 64 1.7649165522650847 1102 65 1.782237574667242 1102 66 1.7669755400259959 1102 67 1.7636469196901032 1102 68 1.8093844802318273 1102 69 1.8451815760934132 1102 70 1.8942593038410642 1102 71 1.7931546547534687 1102 72 1.8194502978985838 1102 73 1.8641781139717786 1102 74 1.9034677660672905 1102 75 1.8610163184811412 1103 1 0.5505106674223548 1103 2 0.5798259795226492 1103 3 0.563639583998075 1103 4 0.5944707445313355 1103 5 0.6584379192389491 1103 6 0.6633670601416028 1103 7 0.7106279989930258 1103 8 0.741443282505081 1103 9 0.8460207259286534 1103 10 0.8887253348269866 1103 11 0.9667853624140506 1103 12 0.918949107060385 1103 13 0.934234165643403 1103 14 0.973588378243889 1103 15 0.9936987748574708 1103 16 0.9783542256100262 1103 17 0.9625961347948078 1103 18 1.017127882595318 1103 19 0.9943682561095031 1103 20 1.0552023953810377 1103 21 1.080995603624224 1103 22 1.1169358398374314 1103 23 1.0984359478830399 1103 24 1.1021252767129965 1103 25 1.1598108295788379 1103 26 1.1049469405570491 1103 27 1.1709307469903578 1103 28 1.1074578476750077 1103 29 1.1042570128091072 1103 30 1.1466435791654987 1103 31 1.1548125743018645 1103 32 1.0985939836203755 1103 33 1.1061970033619701 1103 34 1.1660922338881292 1103 35 1.1430290753211025 1103 36 1.101926920358686 1103 37 1.1420882819814153 1103 38 1.1350030925489776 1103 39 1.0895953569666474 1103 40 1.0963255759047215 1103 41 1.0490759116609993 1103 42 1.0577336994691748 1103 43 1.0961506090888165 1103 44 1.1708311394234698 1103 45 1.1308590345569414 1103 46 1.080317855719393 1103 47 1.1821274372427553 1103 48 1.1007174702179796 1103 49 1.1206945883199353 1103 50 1.0608605835803928 1103 51 1.0917170251299169 1103 52 1.1247576010197378 1103 53 1.0140031623213943 1103 54 1.1622801527538513 1103 55 1.1264413021377848 1103 56 1.2150491897515394 1103 57 1.160978708337609 1103 58 1.110856484604497 1103 59 1.1928998929606323 1103 60 1.1501216085646107 1103 61 1.2084381779405078 1103 62 1.1276124656178155 1103 63 1.101809495393418 1103 64 1.0531205791283398 1103 65 0.9935655686806086 1103 66 1.0597488592534035 1103 67 1.0814083001163723 1103 68 1.0895953908464477 1103 69 1.0640957004199585 1103 70 1.0475649978696957 1103 71 1.0064995266486285 1103 72 1.059719149306595 1103 73 1.0948658381491008 1103 74 1.0194789975662495 1103 75 1.0145834900328232 1104 1 0.44652188039396634 1104 2 0.44095496663327083 1104 3 0.4175055266474601 1104 4 0.5180540559055942 1104 5 0.48834710606268317 1104 6 0.58016504722044 1104 7 0.6506877345202824 1104 8 0.7009377144690205 1104 9 0.7494229441202052 1104 10 0.8084570683774146 1104 11 0.8862801224825034 1104 12 0.789101255273394 1104 13 0.7450300754105612 1104 14 0.8582497179971327 1104 15 0.8011951836510818 1104 16 0.8537032621618934 1104 17 0.9682678723850273 1104 18 0.9337936025437585 1104 19 0.9033563305359067 1104 20 0.9757226871069591 1104 21 0.8644881325820108 1104 22 0.9423662770674497 1104 23 0.9337067725224557 1104 24 0.9514778957559713 1104 25 0.9575338663091131 1104 26 0.975911475535554 1104 27 0.9810521559121312 1104 28 0.9200356930331992 1104 29 0.9474516023657458 1104 30 1.001595916858868 1104 31 1.035224632552719 1104 32 0.9676729840089706 1104 33 1.0201458949326536 1104 34 0.9438909192143683 1104 35 1.0362779764273284 1104 36 1.075935506443571 1104 37 1.0512808065302934 1104 38 1.0137007637246924 1104 39 1.0025668662166325 1104 40 1.0062633488338477 1104 41 0.9845667918936272 1104 42 1.0021183035751946 1104 43 1.0546270557990027 1104 44 1.089481522536751 1104 45 1.050078145824358 1104 46 1.153381726388794 1104 47 1.1104382834550464 1104 48 1.1156766981060429 1104 49 1.0839002924168284 1104 50 1.01410663427869 1104 51 1.0789854628090296 1104 52 0.9559590684671591 1104 53 0.9610209281552571 1104 54 0.9579740271451911 1104 55 0.9777337570669857 1104 56 1.0505943917870155 1104 57 1.0036886875337316 1104 58 0.9436117319696677 1104 59 1.0672010867483834 1104 60 0.9932415953584055 1104 61 1.0615685102796135 1104 62 1.0214250092582802 1104 63 1.0486661619131254 1104 64 1.1007552839727026 1104 65 1.017593318119495 1104 66 1.0244519092924165 1104 67 1.0557687372746187 1104 68 1.1161240348827874 1104 69 0.9997512070902168 1104 70 0.9955745416096171 1104 71 1.074275665043114 1104 72 1.0888521639000714 1104 73 1.0305626039614317 1104 74 1.0418286154782948 1104 75 1.0783016728670098 1105 1 0.4386240479897765 1105 2 0.479304622164193 1105 3 0.4894640111030313 1105 4 0.46359081620331466 1105 5 0.4737043321455374 1105 6 0.5358139217954587 1105 7 0.5937963413674439 1105 8 0.6240698635218536 1105 9 0.7672261097700925 1105 10 0.7618265316270723 1105 11 0.7294727801824195 1105 12 0.7630027484706545 1105 13 0.6758890472415757 1105 14 0.7976437551667317 1105 15 0.7076405695181904 1105 16 0.7265864653359131 1105 17 0.7085722081741608 1105 18 0.7073259890103785 1105 19 0.8008199068399406 1105 20 0.7944428037424558 1105 21 0.8611718285091285 1105 22 0.8642815609780854 1105 23 0.8857641262749709 1105 24 0.8680644352708313 1105 25 0.8574505324778485 1105 26 0.8894619468776028 1105 27 0.9052354983078246 1105 28 0.9426721013928727 1105 29 0.9231140605145924 1105 30 0.8820541334272392 1105 31 0.8902418908617022 1105 32 0.9685548407759654 1105 33 0.8876291295759486 1105 34 0.9358952532109512 1105 35 1.0209158307803463 1105 36 0.9414236686179009 1105 37 1.0129004784132931 1105 38 1.0076786197109229 1105 39 1.0569278506871456 1105 40 0.9535937383956039 1105 41 0.9344781401529545 1105 42 1.0119747996903357 1105 43 1.0331597111486843 1105 44 1.0483027790372503 1105 45 1.0355324884849466 1105 46 1.041315728543843 1105 47 1.0467304856471387 1105 48 0.9565256913879523 1105 49 0.9905393537560272 1105 50 0.9459016378339271 1105 51 0.9181751488532015 1105 52 0.9250622538351365 1105 53 0.8838115758049199 1105 54 0.9380824168941757 1105 55 0.9408516851935502 1105 56 0.9583551535825166 1105 57 0.9355684589632105 1105 58 0.9144762009654599 1105 59 0.8701449778248502 1105 60 0.9130799070117916 1105 61 0.933625625024959 1105 62 0.9211873166334428 1105 63 0.8950407618250864 1105 64 0.8990903202320837 1105 65 0.8527181694869199 1105 66 0.8406849572113586 1105 67 0.8765263501507334 1105 68 0.9125461374686523 1105 69 0.8468658130171995 1105 70 0.8430770561641516 1105 71 0.8051894491837892 1105 72 0.8388024703271668 1105 73 0.9139445155008907 1105 74 0.9850536984834939 1105 75 0.9579577017231422 1106 1 0.29555924709059767 1106 2 0.3265406663930648 1106 3 0.3111664018936793 1106 4 0.3781038650687094 1106 5 0.36056155071998575 1106 6 0.4248818865490449 1106 7 0.46515125446605055 1106 8 0.46855154117832143 1106 9 0.6305262121711124 1106 10 0.5414911537603402 1106 11 0.5393992862387265 1106 12 0.5436287313635191 1106 13 0.4601485809391832 1106 14 0.49083414551104454 1106 15 0.5540989006721588 1106 16 0.6203619711516168 1106 17 0.6767078238530218 1106 18 0.5335849345704631 1106 19 0.6372050462552679 1106 20 0.6566711243076284 1106 21 0.6640537023664166 1106 22 0.6366645524128955 1106 23 0.6618479672546727 1106 24 0.6299667230792991 1106 25 0.7088896677994612 1106 26 0.6884569988510592 1106 27 0.7423179177614543 1106 28 0.7400355947902781 1106 29 0.6045743618130288 1106 30 0.6182850320339597 1106 31 0.575500793415685 1106 32 0.6932439473299752 1106 33 0.6438041667413614 1106 34 0.7293095077582592 1106 35 0.6662053257840839 1106 36 0.7564043216830143 1106 37 0.6693816725416823 1106 38 0.6807521496867608 1106 39 0.7296765102333964 1106 40 0.6586337050135711 1106 41 0.6449343186771017 1106 42 0.635486120175301 1106 43 0.7520803044469346 1106 44 0.7587604685866509 1106 45 0.783778711212392 1106 46 0.6944666230126799 1106 47 0.7648495381645688 1106 48 0.6972838541207196 1106 49 0.724686432304928 1106 50 0.7361648235633353 1106 51 0.7283959561358841 1106 52 0.6811673189080238 1106 53 0.7461329987722678 1106 54 0.7402238883770593 1106 55 0.7782404940755896 1106 56 0.7363860087182061 1106 57 0.6769946110909117 1106 58 0.6851685876065972 1106 59 0.716164181793502 1106 60 0.6565697709381553 1106 61 0.6839743812768262 1106 62 0.6573620748403428 1106 63 0.5903644873624643 1106 64 0.6549601302962031 1106 65 0.7170263581976997 1106 66 0.7270049481917056 1106 67 0.6885913056394131 1106 68 0.8056602791539973 1106 69 0.7995574720415668 1106 70 0.821093730908995 1106 71 0.7500779413316927 1106 72 0.8709591799185787 1106 73 0.8267607992388939 1106 74 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-2.2909330583115164 2216 40 -2.2855246142559196 2216 41 -2.2177683770883476 2216 42 -2.1674301748423517 2216 43 -2.346042734369263 2216 44 -2.3323167467088197 2216 45 -2.3448894912470166 2216 46 -2.2619955778767107 2216 47 -2.3008545843052772 2216 48 -2.379625993989805 2216 49 -2.278206761652001 2216 50 -2.325452550145517 2216 51 -2.365104731548474 2216 52 -2.298173060248832 2216 53 -2.366245961294979 2216 54 -2.3956779406708115 2216 55 -2.468447177444439 2216 56 -2.5886069860990304 2216 57 -2.4948507572533636 2216 58 -2.534521644280993 2216 59 -2.4598624785417655 2216 60 -2.4759845432918794 2216 61 -2.375261871987803 2216 62 -2.304184498395365 2216 63 -2.317662917340318 2216 64 -2.3338927523097652 2216 65 -2.3254582036628797 2216 66 -2.3454519565656184 2216 67 -2.284561839420274 2216 68 -2.408768900052479 2216 69 -2.5361826495812423 2216 70 -2.3423839198842487 2216 71 -2.3009013939138967 2216 72 -2.3612077637469184 2216 73 -2.29926660974186 2216 74 -2.3058188320237853 2216 75 -2.2442895606029047 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 78004.0 3 29876.0 4 17244.0 5 10949.0 6 8103.0 7 7244.0 8 6989.0 9 7154.0 10 7622.0 11 8214.0 12 8694.0 13 9061.0 14 9551.0 15 9882.0 16 10718.0 17 11188.0 18 11999.0 19 13014.0 20 14361.0 21 16768.0 22 20546.0 23 24447.0 24 26093.0 25 27573.0 26 29165.0 27 31256.0 28 36273.0 29 43052.0 30 50130.0 31 57522.0 32 69901.0 33 87273.0 34 113729.0 35 154018.0 36 219252.0 37 350244.0 38 590294.0 39 853326.0 40 199329.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.725902077070764 17.92954354843778 14.106544720891614 26.238009653599843 2 15.607122187956046 19.407699949444346 37.32263594378992 27.66254191880969 3 18.49938338132644 23.15019733321951 28.915772480666835 29.434646804787217 4 12.762473241314801 15.834333667524328 35.26703122385953 36.13616186730135 5 14.338419736268726 36.82603940466992 33.847680114447115 14.987860744614235 6 34.14639131854085 35.660887366182706 16.11411957446938 14.078601740807068 7 29.374211680666324 31.244891934685075 20.322140520284663 19.058755864363942 8 27.226116794055148 34.43313141465866 18.719520079238645 19.621231712047543 9 26.287258739548065 15.490204391758445 18.423594743750034 39.79894212494346 10 14.732602202662786 28.866782022748772 32.80637316940327 23.594242605185165 11 35.926854465132955 22.497761883969233 22.27056566400695 19.30481798689086 12 23.841938984656437 24.289067850302317 29.40655858178573 22.46243458325551 13 28.652882800110945 21.251899098410753 25.34919152340724 24.746026578071064 14 22.366205637762075 20.508109895365635 26.437807461054934 30.68787700581736 15 24.065953266636438 28.44176686498468 23.232511428112286 24.259768440266598 16 24.018969197926197 27.68310460506252 24.64077462641049 23.657151570600792 17 22.82332922679092 27.546960955320003 26.068910627813086 23.56079919007599 18 22.965867259940136 26.682974368732147 27.16211221943312 23.189046151894598 19 23.66789120176411 26.34711057283606 27.394002705198055 22.59099552020178 20 23.75203490384155 25.766037054688027 27.46360193911586 23.018326102354557 21 23.808672830686856 25.65275558782692 27.479821672592514 23.05874990889371 22 23.672104334430745 25.352131143142113 27.43794768215333 23.537816840273805 23 23.531153370368372 25.431539301932528 27.398244357462215 23.639062970236893 24 23.26984686524764 25.552139550286736 27.388975932308206 23.78903765215742 25 23.507577714144407 25.477420537140606 27.5009597350369 23.51404201367809 26 23.296918587257085 25.764604452051138 27.526522705363217 23.411954255328556 27 23.442001030767496 25.810143606039443 27.304847961794508 23.44300740139855 28 23.350897530795294 25.73546836385795 27.4071627401124 23.506471365234358 29 23.48171271456364 25.793543065851065 27.294733586343085 23.430010633242212 30 23.525868607644583 25.78275560348495 27.319827368776757 23.371548420093706 31 23.529151674086723 25.43376837226322 27.352381533207293 23.684698420442764 32 23.281132800351255 25.78438008817987 27.290176420726535 23.64431069074233 33 23.4867359661274 25.650852562658116 27.20952633307344 23.652885138141038 34 23.6200307232402 25.683238392951342 27.27001630075845 23.426714583050014 35 23.554932901201724 25.78322583399012 27.23889550332102 23.422945761487135 36 23.68612017006164 25.667885864540978 27.055277350238676 23.59071661515871 37 23.526680238686232 25.47668491886821 27.290613689242626 23.706021153202936 38 23.577733495610946 25.620797225345555 27.209863730710225 23.59160554833328 39 23.710928436423995 25.803597372143216 27.01486653232851 23.470607659104274 40 23.602435232386505 25.629488423742874 27.27028524368332 23.497791100187303 41 23.402578316044956 25.677539744973565 27.165407389790403 23.754474549191073 42 23.69975065507163 25.695973588585137 27.036057752407622 23.568218003935613 43 23.592871774013062 25.552566794341182 27.05136515784704 23.80319627379871 44 23.636495576311336 25.79722288030505 26.84959641482574 23.716685128557877 45 23.642132683879865 25.82260481004523 26.71711760713635 23.818144898938563 46 23.762237079991095 25.681450248323927 26.616209195759744 23.94010347592523 47 23.600703436509306 25.874005837986424 26.639212560878416 23.88607816462585 48 23.53140432294028 26.018316075943694 26.61383093317349 23.836448667942538 49 23.745807162077536 25.779741329110568 26.520686902857864 23.953764605954028 50 23.830330686931394 25.97243152621712 26.436875524191546 23.76036226265994 51 23.640678515918683 26.096576139747107 26.218467052563092 24.04427829177111 52 23.772926360642423 26.125761366456523 26.215122266809516 23.886190006091542 53 23.720167058072466 26.1105523471086 26.3574383006149 23.811842294204034 54 23.579491582161047 26.10405059910526 26.46239792101237 23.85405989772132 55 23.650911099749486 25.908592163465947 26.40251801269921 24.037978724085356 56 23.510703591422246 26.192066740919458 26.35012578560742 23.947103882050875 57 23.664050688253035 25.984437057522765 26.27297619399684 24.078536060227364 58 23.612954326569103 25.85496488312515 26.45815745932909 24.07392333097666 59 23.58251367453092 25.996335164734653 26.463281521546918 23.957869639187514 60 23.558018292682927 26.081189024390245 26.541859756097562 23.818932926829266 61 23.601848399418664 25.930548742434134 26.571275175478736 23.896327682668467 62 23.50373893733796 26.11575001052062 26.420573578176143 23.959937473965272 63 23.374874341828644 26.01481645903277 26.573429345272526 24.036879853866065 64 23.485078319878305 25.890579325918207 26.554330880561615 24.070011473641877 65 23.56357817207819 25.847375063692063 26.516541945587846 24.072504818641903 66 23.439746934563775 26.029953638821908 26.42059626744338 24.10970315917094 67 23.498621430309505 25.922598846282618 26.455883635423355 24.12289608798452 68 23.584583761374144 25.96090779108986 26.51559989595902 23.938908551576972 69 23.50965644266427 26.048750682580902 26.317849913608356 24.123742961146473 70 23.379130462358088 25.778738211747026 26.56702045655239 24.27511086934249 71 23.512097317595064 25.892808686335645 26.379847906194797 24.215246089874498 72 23.357288979863903 26.041878523558083 26.325912866252605 24.274919630325414 73 23.493261971628296 25.878303576083667 26.37573986597423 24.252694586313808 74 23.42860122479012 26.009485216741133 26.230499975757898 24.33141358271084 75 23.570986281431715 26.04592002395117 26.24104155875277 24.142052135864343 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 757.0 1 547.0 2 406.0 3 496.0 4 517.0 5 414.0 6 396.5 7 788.5 8 1095.0 9 1857.0 10 3040.5 11 3460.5 12 3459.0 13 3237.0 14 3181.0 15 3615.5 16 3884.0 17 4232.0 18 4989.0 19 6021.0 20 6644.0 21 7527.0 22 9981.0 23 12941.0 24 14330.0 25 16689.5 26 21915.5 27 27276.0 28 29770.0 29 33822.5 30 41114.5 31 48677.5 32 53001.0 33 56666.5 34 66204.5 35 76782.0 36 81487.0 37 85214.0 38 92655.0 39 101016.0 40 105663.0 41 111237.5 42 120694.0 43 129586.5 44 134597.0 45 140665.0 46 149809.5 47 157300.5 48 161715.0 49 164664.0 50 168891.5 51 170573.0 52 170976.0 53 168594.5 54 164465.5 55 157256.5 56 151795.0 57 146272.5 58 132154.0 59 113027.0 60 102496.0 61 93122.5 62 74122.5 63 56300.0 64 48104.0 65 39941.0 66 26713.0 67 18772.0 68 15896.0 69 13270.0 70 8856.0 71 5892.5 72 4717.0 73 4229.0 74 3192.0 75 2036.0 76 1429.0 77 1198.0 78 748.5 79 419.0 80 308.0 81 247.0 82 129.5 83 55.5 84 38.0 85 31.5 86 18.5 87 10.5 88 9.0 89 7.5 90 4.0 91 2.0 92 2.0 93 2.5 94 1.5 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07063899479826272 2 0.015365581950075273 3 0.0022255704014989977 4 0.13676587426197953 5 0.09408370217843709 6 0.06914511877533873 7 0.028353157169781755 8 0.02591417590786504 9 0.04874913797256024 10 0.02853608076442551 11 0.01567045460781486 12 0.10286403472133723 13 0.086736271126913 14 0.04859670164369045 15 0.019664286424203474 16 0.03225552718884849 17 0.02249960214118165 18 0.019085028374498256 19 0.01359732053518566 20 0.012774164359288768 21 0.028078771777816123 22 0.016066789062876327 23 0.014054629521795042 24 0.022072780420346223 25 0.01545704374739715 26 0.03277381070700579 27 0.028901927953713016 28 0.037468849636195456 29 0.05060886118477174 30 0.03539571556356625 31 0.02006062087926494 32 0.012133931778035633 33 0.014481451242630465 34 0.013902193192925245 35 0.005884042294374063 36 0.009329103326831416 37 0.008262049024742856 38 0.0024999557934646277 39 0.00838399808783869 40 0.011310775602138742 41 0.012133931778035633 42 0.008048638164325142 43 0.01109736474172103 44 0.01003031043963247 45 0.005243809713120926 46 0.0020731340726292033 47 0.00487796252383342 48 0.0063108640152094875 49 0.0018292359464375325 50 2.1341086041771213E-4 51 0.002317032198820875 52 0.0030487265773958875 53 3.0487265773958872E-5 54 0.0 55 3.353599235135476E-4 56 0.008262049024742856 57 0.002652392122334422 58 3.353599235135476E-4 59 0.007255969254202212 60 0.0017682614148896148 61 9.451052389927253E-4 62 0.023902016366783758 63 0.009969335908084553 64 0.011188826539042906 65 0.018505770324793037 66 0.017987486806635734 67 0.017438716022704477 68 0.017713101414670106 69 0.006920609330688664 70 0.015914352734006532 71 0.00731694378575013 72 0.01600581453132841 73 0.007347431051524089 74 0.01948136282955972 75 0.0015548505544719026 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 3280058.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.989505762350305 #Duplication Level Percentage of deduplicated Percentage of total 1 77.02533505378601 46.977371161098446 2 12.85017257874102 15.674513490766426 3 4.3557310219565695 7.969616467886046 4 1.9949978301856914 4.866957266399464 5 1.088234931820337 3.3185455322523665 6 0.6610739177565041 2.419114290980988 7 0.44516104525286915 1.900510649423662 8 0.28217509936380597 1.376777587891249 9 0.2268800612692246 1.2453572523727587 >10 1.0071405340929573 10.533164435241698 >50 0.04805176536754134 1.9864334782602082 >100 0.014443866694496522 1.4225557897631544 >500 4.5172028522389134E-4 0.17231117609478735 >1k 1.5057342778405458E-4 0.13677142156862732 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.097453154791775E-5 2 0.0 0.0 0.0 0.0 6.097453154791775E-5 3 0.0 0.0 0.0 0.0 6.097453154791775E-5 4 0.0 0.0 0.0 0.0 6.097453154791775E-5 5 0.0 0.0 0.0 0.0 6.097453154791775E-5 6 0.0 0.0 0.0 0.0 2.1341086041771213E-4 7 0.0 0.0 0.0 0.0 2.1341086041771213E-4 8 0.0 0.0 0.0 0.0 2.1341086041771213E-4 9 0.0 0.0 0.0 0.0 2.43898126191671E-4 10 0.0 0.0 0.0 0.0 2.43898126191671E-4 11 0.0 0.0 0.0 0.0 2.43898126191671E-4 12 0.0 0.0 0.0 0.0 2.7438539196562986E-4 13 0.0 0.0 0.0 0.0 2.7438539196562986E-4 14 0.0 0.0 0.0 0.0 2.7438539196562986E-4 15 0.0 0.0 0.0 3.0487265773958876E-5 3.0487265773958874E-4 16 0.0 0.0 0.0 3.0487265773958876E-5 3.0487265773958874E-4 17 0.0 0.0 0.0 3.0487265773958876E-5 3.0487265773958874E-4 18 0.0 0.0 0.0 3.0487265773958876E-5 3.353599235135476E-4 19 0.0 0.0 0.0 3.0487265773958876E-5 3.353599235135476E-4 20 0.0 0.0 0.0 3.0487265773958876E-5 3.658471892875065E-4 21 0.0 0.0 0.0 3.0487265773958876E-5 3.658471892875065E-4 22 0.0 0.0 0.0 1.219490630958355E-4 3.963344550614654E-4 23 0.0 0.0 0.0 1.8292359464375325E-4 4.2682172083542425E-4 24 0.0 0.0 0.0 2.43898126191671E-4 4.87796252383342E-4 25 0.0 0.0 0.0 3.0487265773958874E-4 4.87796252383342E-4 26 0.0 0.0 0.0 5.182835181573008E-4 4.87796252383342E-4 27 0.0 0.0 0.0 8.231561758968896E-4 5.182835181573008E-4 28 0.0 0.0 0.0 0.0014938760229239849 5.182835181573008E-4 29 0.0 0.0 0.0 0.0024999557934646277 5.487707839312597E-4 30 0.0 0.0 0.0 0.0044511408029979955 5.487707839312597E-4 31 0.0 0.0 0.0 0.008841307074448074 5.487707839312597E-4 32 0.0 0.0 0.0 0.014085116787569 5.792580497052186E-4 33 0.0 0.0 0.0 0.02106670064980558 5.792580497052186E-4 34 0.0 0.0 0.0 0.02981654592693178 6.402325812531364E-4 35 3.0487265773958876E-5 0.0 0.0 0.04067001254246114 6.402325812531364E-4 36 3.0487265773958876E-5 0.0 0.0 0.05350515143329783 6.402325812531364E-4 37 3.0487265773958876E-5 0.0 0.0 0.07127922737951585 6.707198470270952E-4 38 3.0487265773958876E-5 0.0 0.0 0.09292518607902665 6.707198470270952E-4 39 3.0487265773958876E-5 0.0 0.0 0.11396139946305828 7.012071128010541E-4 40 3.0487265773958876E-5 0.0 0.0 0.13493663831554198 7.31694378575013E-4 41 3.0487265773958876E-5 0.0 0.0 0.15539359364986838 7.31694378575013E-4 42 3.0487265773958876E-5 0.0 0.0 0.177923683056824 7.31694378575013E-4 43 3.0487265773958876E-5 0.0 0.0 0.20115497957658066 7.31694378575013E-4 44 3.0487265773958876E-5 0.0 0.0 0.23002642026451972 7.31694378575013E-4 45 3.0487265773958876E-5 0.0 0.0 0.261702689403663 7.31694378575013E-4 46 3.0487265773958876E-5 0.0 0.0 0.2956045289443053 7.621816443489719E-4 47 3.0487265773958876E-5 0.0 0.0 0.33212827334150796 8.536434416708485E-4 48 3.0487265773958876E-5 0.0 0.0 0.3688349413333545 8.841307074448074E-4 49 3.0487265773958876E-5 0.0 0.0 0.40691353628502913 8.841307074448074E-4 50 3.0487265773958876E-5 0.0 0.0 0.4431019207587183 8.841307074448074E-4 51 3.0487265773958876E-5 0.0 0.0 0.48218659548093357 9.146179732187663E-4 52 3.0487265773958876E-5 0.0 0.0 0.5207834739507655 9.75592504766684E-4 53 3.0487265773958876E-5 0.0 0.0 0.5587096325735703 0.0011585160994104372 54 3.0487265773958876E-5 3.0487265773958876E-5 0.0 0.6009040084047295 0.001189003365184396 55 3.0487265773958876E-5 6.097453154791775E-5 0.0 0.649409248251098 0.001189003365184396 56 3.0487265773958876E-5 6.097453154791775E-5 0.0 0.7045607120361896 0.001219490630958355 57 3.0487265773958876E-5 6.097453154791775E-5 0.0 0.7630352877906428 0.001219490630958355 58 3.0487265773958876E-5 6.097453154791775E-5 0.0 0.8219061980001573 0.001219490630958355 59 3.0487265773958876E-5 6.097453154791775E-5 0.0 0.8834599875977803 0.0012804651625062727 60 3.0487265773958876E-5 6.097453154791775E-5 0.0 0.9495258925299491 0.0012804651625062727 61 3.0487265773958876E-5 6.097453154791775E-5 0.0 1.0166893390299805 0.0013109524282802316 62 3.0487265773958876E-5 6.097453154791775E-5 0.0 1.0790967720692743 0.0013109524282802316 63 3.0487265773958876E-5 6.097453154791775E-5 0.0 1.1470224002136549 0.0013109524282802316 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3290 0.0 40.158787 1 GTATCAA 6355 0.0 33.63458 1 TCAACGC 8795 0.0 23.66507 4 TATCAAC 8940 0.0 23.663992 2 ATCAACG 8825 0.0 23.544724 3 CAACGCA 8950 0.0 23.071789 5 AACGCAG 9435 0.0 21.961006 6 CGTGCCG 450 0.0 19.952185 10 ACGCAGA 11010 0.0 18.817434 7 GTACATG 7895 0.0 18.79396 1 CGCAGAG 11250 0.0 18.29406 8 GTGGTAT 1565 0.0 17.901268 1 GAGTACT 7555 0.0 17.82624 12 AGAGTAC 11150 0.0 17.746365 11 TACATGG 8230 0.0 17.72502 2 CGCACGA 365 0.0 17.007717 35 AGTACTT 7980 0.0 16.824583 13 CAGAGTA 12055 0.0 16.614618 10 ACATGGG 8400 0.0 16.463211 3 GCAGAGT 12455 0.0 16.385765 9 >>END_MODULE