FastQCFastQC Report
Mon 6 Feb 2017
SRR2077484_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077484_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3404836
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33750.038.9717751
GTATCAA64350.033.6370541
TCAACGC81600.025.961384
ATCAACG83100.025.5339093
TATCAAC83250.025.527842
CAACGCA82750.025.351545
AACGCAG86850.024.1724156
ACGCAGA101200.020.5075657
GTACATG89500.020.0576151
CGCAGAG104550.019.753868
AGAGTAC99300.019.68918611
TACATGG91400.018.986262
GAGTACT60200.018.7881512
CAGAGTA110000.018.11851910
ACATGGG93500.017.8598143
GCAGAGT114000.017.5732639
AGTACTT66150.016.94282313
GTACTTT66600.016.77705814
GTGTAGC14100.015.669671
TAGGACC9300.015.2107454
Produced by FastQC (version 0.11.5)