FastQCFastQC Report
Mon 6 Feb 2017
SRR2077484_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077484_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3404836
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38650.037.1512371
GTATCAA73300.032.3341371
TATCAAC99050.023.8764212
ATCAACG99250.023.8652523
TCAACGC100000.023.7204914
CAACGCA100850.023.4541595
GTGGTAT18500.023.37831
AACGCAG105350.022.5479976
CGTGCCG4050.021.31492410
TGGTATC19350.020.7273882
GTACATG85550.019.9389171
GCACCGT6750.019.437216
ACGCAGA121000.019.4330677
CGCAGAG124850.018.7231858
TACATGG91100.018.4832422
CGAATCG2801.2732926E-1118.47602837
GAGTACT85100.018.1780512
AGAGTAC121750.018.09466211
GGACCGT3500.017.7565486
ACATGGG93050.017.6515663
Produced by FastQC (version 0.11.5)